Cargando…

Relationship between the Length of Sperm Tail Mitochondrial Sheath and Fertility Traits in Boars Used for Artificial Insemination

The length of sperm tail midpiece, occupied by the mitochondrial sheath (MS), has been correlated with reproductive traits of mice, fish, and birds; however, it is not known whether such a correlation exists in higher order species such as domestic pigs. As the mitochondria provide for sperm motilit...

Descripción completa

Detalles Bibliográficos
Autores principales: Kerns, Karl, Jankovitz, Jennifer, Robinson, Julie, Minton, Amanda, Kuster, Chris, Sutovsky, Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7690677/
https://www.ncbi.nlm.nih.gov/pubmed/33113996
http://dx.doi.org/10.3390/antiox9111033
_version_ 1783614122737270784
author Kerns, Karl
Jankovitz, Jennifer
Robinson, Julie
Minton, Amanda
Kuster, Chris
Sutovsky, Peter
author_facet Kerns, Karl
Jankovitz, Jennifer
Robinson, Julie
Minton, Amanda
Kuster, Chris
Sutovsky, Peter
author_sort Kerns, Karl
collection PubMed
description The length of sperm tail midpiece, occupied by the mitochondrial sheath (MS), has been correlated with reproductive traits of mice, fish, and birds; however, it is not known whether such a correlation exists in higher order species such as domestic pigs. As the mitochondria provide for sperm motility and generate the fertility-affecting reactive oxygen species (ROS), we hypothesized that MS length correlates with boar semen parameters and artificial insemination (AI) fertility. Sperm samples collected from 57 boars and used for single sire AI were labeled with ProteoStat Aggresome probe (AGG; Enzo Life Sciences) for MS imaging by epifluorescence microscopy and image-based flow cytometry (IBFC). The mean boar MS length was 7.26 ± 0.2 µm, ranging from 6.94 ± 0.18 µm to 7.65 ± 0.31 µm. The absolute longest MS measured was 9.19 µm and the shortest was 5.83 µm. Boars in the high tertile of MS length had significantly higher conception rate (CR; p = 0.05) and sperm parameters. Boars within the high tertile of average number piglets born per litter had significantly shorter MS and more varied MS length than boars in the low tertile (p = 0.04). MS length data correlated with conventional sperm parameters including percent viable and intact acrosomes (p = 0.03), basal:induced oxidation ratio (measure of intracellular ROS levels; p = 0.02) and Comp DNA (chromatin integrity; p = 0.06) along with many flow cytometric AGG parameters in IBFC. Sperm head AGG intensity median absolute deviation had a negative correlation with total born (r = −0.423 p = 0.004). These data reveal a complex relationship between sperm MS length and aggresome abundance to sperm parameters and boar reproductive success in AI service.
format Online
Article
Text
id pubmed-7690677
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-76906772020-11-27 Relationship between the Length of Sperm Tail Mitochondrial Sheath and Fertility Traits in Boars Used for Artificial Insemination Kerns, Karl Jankovitz, Jennifer Robinson, Julie Minton, Amanda Kuster, Chris Sutovsky, Peter Antioxidants (Basel) Article The length of sperm tail midpiece, occupied by the mitochondrial sheath (MS), has been correlated with reproductive traits of mice, fish, and birds; however, it is not known whether such a correlation exists in higher order species such as domestic pigs. As the mitochondria provide for sperm motility and generate the fertility-affecting reactive oxygen species (ROS), we hypothesized that MS length correlates with boar semen parameters and artificial insemination (AI) fertility. Sperm samples collected from 57 boars and used for single sire AI were labeled with ProteoStat Aggresome probe (AGG; Enzo Life Sciences) for MS imaging by epifluorescence microscopy and image-based flow cytometry (IBFC). The mean boar MS length was 7.26 ± 0.2 µm, ranging from 6.94 ± 0.18 µm to 7.65 ± 0.31 µm. The absolute longest MS measured was 9.19 µm and the shortest was 5.83 µm. Boars in the high tertile of MS length had significantly higher conception rate (CR; p = 0.05) and sperm parameters. Boars within the high tertile of average number piglets born per litter had significantly shorter MS and more varied MS length than boars in the low tertile (p = 0.04). MS length data correlated with conventional sperm parameters including percent viable and intact acrosomes (p = 0.03), basal:induced oxidation ratio (measure of intracellular ROS levels; p = 0.02) and Comp DNA (chromatin integrity; p = 0.06) along with many flow cytometric AGG parameters in IBFC. Sperm head AGG intensity median absolute deviation had a negative correlation with total born (r = −0.423 p = 0.004). These data reveal a complex relationship between sperm MS length and aggresome abundance to sperm parameters and boar reproductive success in AI service. MDPI 2020-10-23 /pmc/articles/PMC7690677/ /pubmed/33113996 http://dx.doi.org/10.3390/antiox9111033 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kerns, Karl
Jankovitz, Jennifer
Robinson, Julie
Minton, Amanda
Kuster, Chris
Sutovsky, Peter
Relationship between the Length of Sperm Tail Mitochondrial Sheath and Fertility Traits in Boars Used for Artificial Insemination
title Relationship between the Length of Sperm Tail Mitochondrial Sheath and Fertility Traits in Boars Used for Artificial Insemination
title_full Relationship between the Length of Sperm Tail Mitochondrial Sheath and Fertility Traits in Boars Used for Artificial Insemination
title_fullStr Relationship between the Length of Sperm Tail Mitochondrial Sheath and Fertility Traits in Boars Used for Artificial Insemination
title_full_unstemmed Relationship between the Length of Sperm Tail Mitochondrial Sheath and Fertility Traits in Boars Used for Artificial Insemination
title_short Relationship between the Length of Sperm Tail Mitochondrial Sheath and Fertility Traits in Boars Used for Artificial Insemination
title_sort relationship between the length of sperm tail mitochondrial sheath and fertility traits in boars used for artificial insemination
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7690677/
https://www.ncbi.nlm.nih.gov/pubmed/33113996
http://dx.doi.org/10.3390/antiox9111033
work_keys_str_mv AT kernskarl relationshipbetweenthelengthofspermtailmitochondrialsheathandfertilitytraitsinboarsusedforartificialinsemination
AT jankovitzjennifer relationshipbetweenthelengthofspermtailmitochondrialsheathandfertilitytraitsinboarsusedforartificialinsemination
AT robinsonjulie relationshipbetweenthelengthofspermtailmitochondrialsheathandfertilitytraitsinboarsusedforartificialinsemination
AT mintonamanda relationshipbetweenthelengthofspermtailmitochondrialsheathandfertilitytraitsinboarsusedforartificialinsemination
AT kusterchris relationshipbetweenthelengthofspermtailmitochondrialsheathandfertilitytraitsinboarsusedforartificialinsemination
AT sutovskypeter relationshipbetweenthelengthofspermtailmitochondrialsheathandfertilitytraitsinboarsusedforartificialinsemination