Cargando…
Transcriptomic and Phenotypic Analyses of the Sigma B-Dependent Characteristics and the Synergism between Sigma B and Sigma L in Listeria monocytogenes EGD-e
Numerous gene expression and stress adaptation responses in L. monocytogenes are regulated through alternative sigma factors σ(B) and σ(L). Stress response phenotypes and transcriptomes were compared between L. monocytogenes EGD-e and its ΔsigB and ΔsigBL mutants. Targeted growth phenotypic analysis...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7690807/ https://www.ncbi.nlm.nih.gov/pubmed/33114171 http://dx.doi.org/10.3390/microorganisms8111644 |
_version_ | 1783614152513683456 |
---|---|
author | Mattila, Mirjami Somervuo, Panu Korkeala, Hannu Stephan, Roger Tasara, Taurai |
author_facet | Mattila, Mirjami Somervuo, Panu Korkeala, Hannu Stephan, Roger Tasara, Taurai |
author_sort | Mattila, Mirjami |
collection | PubMed |
description | Numerous gene expression and stress adaptation responses in L. monocytogenes are regulated through alternative sigma factors σ(B) and σ(L). Stress response phenotypes and transcriptomes were compared between L. monocytogenes EGD-e and its ΔsigB and ΔsigBL mutants. Targeted growth phenotypic analysis revealed that the ΔsigB and ΔsigBL mutants are impaired during growth under cold and organic-acid stress conditions. Phenotypic microarrays revealed increased sensitivity in both mutants to various antimicrobial compounds. Genes de-regulated in these two mutants were identified by genome-wide transcriptome analysis during exponential growth in BHI. The ΔsigB and ΔsigBL strains repressed 198 and 254 genes, respectively, compared to the parent EGD-e strain at 3 °C, whereas 86 and 139 genes, respectively, were repressed in these mutants during growth at 37 °C. Genes repressed in these mutants are involved in various cellular functions including transcription regulation, energy metabolism and nutrient transport functions, and viral-associated processes. Exposure to cold stress induced a significant increase in σ(B) and σ(L) co-dependent genes of L. monocytogenes EGD-e since most (62%) of the down-regulated genes uncovered at 3 °C were detected in the ΔsigBL double-deletion mutant but not in ΔsigB or ΔsigL single-deletion mutants. Overall, the current study provides an expanded insight into σ(B) and σ(L) phenotypic roles and functional interactions in L. monocytogenes. Besides previously known σ(B)- and σ(L)-dependent genes, the transcriptomes defined in ΔsigB and ΔsigBL mutants reveal several new genes that are positively regulated by σ(B) alone, as well as those co-regulated through σ(B)- and σ(L)-dependent mechanisms during L. monocytogenes growth under optimal and cold-stress temperature conditions. |
format | Online Article Text |
id | pubmed-7690807 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-76908072020-11-27 Transcriptomic and Phenotypic Analyses of the Sigma B-Dependent Characteristics and the Synergism between Sigma B and Sigma L in Listeria monocytogenes EGD-e Mattila, Mirjami Somervuo, Panu Korkeala, Hannu Stephan, Roger Tasara, Taurai Microorganisms Article Numerous gene expression and stress adaptation responses in L. monocytogenes are regulated through alternative sigma factors σ(B) and σ(L). Stress response phenotypes and transcriptomes were compared between L. monocytogenes EGD-e and its ΔsigB and ΔsigBL mutants. Targeted growth phenotypic analysis revealed that the ΔsigB and ΔsigBL mutants are impaired during growth under cold and organic-acid stress conditions. Phenotypic microarrays revealed increased sensitivity in both mutants to various antimicrobial compounds. Genes de-regulated in these two mutants were identified by genome-wide transcriptome analysis during exponential growth in BHI. The ΔsigB and ΔsigBL strains repressed 198 and 254 genes, respectively, compared to the parent EGD-e strain at 3 °C, whereas 86 and 139 genes, respectively, were repressed in these mutants during growth at 37 °C. Genes repressed in these mutants are involved in various cellular functions including transcription regulation, energy metabolism and nutrient transport functions, and viral-associated processes. Exposure to cold stress induced a significant increase in σ(B) and σ(L) co-dependent genes of L. monocytogenes EGD-e since most (62%) of the down-regulated genes uncovered at 3 °C were detected in the ΔsigBL double-deletion mutant but not in ΔsigB or ΔsigL single-deletion mutants. Overall, the current study provides an expanded insight into σ(B) and σ(L) phenotypic roles and functional interactions in L. monocytogenes. Besides previously known σ(B)- and σ(L)-dependent genes, the transcriptomes defined in ΔsigB and ΔsigBL mutants reveal several new genes that are positively regulated by σ(B) alone, as well as those co-regulated through σ(B)- and σ(L)-dependent mechanisms during L. monocytogenes growth under optimal and cold-stress temperature conditions. MDPI 2020-10-23 /pmc/articles/PMC7690807/ /pubmed/33114171 http://dx.doi.org/10.3390/microorganisms8111644 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Mattila, Mirjami Somervuo, Panu Korkeala, Hannu Stephan, Roger Tasara, Taurai Transcriptomic and Phenotypic Analyses of the Sigma B-Dependent Characteristics and the Synergism between Sigma B and Sigma L in Listeria monocytogenes EGD-e |
title | Transcriptomic and Phenotypic Analyses of the Sigma B-Dependent Characteristics and the Synergism between Sigma B and Sigma L in Listeria monocytogenes EGD-e |
title_full | Transcriptomic and Phenotypic Analyses of the Sigma B-Dependent Characteristics and the Synergism between Sigma B and Sigma L in Listeria monocytogenes EGD-e |
title_fullStr | Transcriptomic and Phenotypic Analyses of the Sigma B-Dependent Characteristics and the Synergism between Sigma B and Sigma L in Listeria monocytogenes EGD-e |
title_full_unstemmed | Transcriptomic and Phenotypic Analyses of the Sigma B-Dependent Characteristics and the Synergism between Sigma B and Sigma L in Listeria monocytogenes EGD-e |
title_short | Transcriptomic and Phenotypic Analyses of the Sigma B-Dependent Characteristics and the Synergism between Sigma B and Sigma L in Listeria monocytogenes EGD-e |
title_sort | transcriptomic and phenotypic analyses of the sigma b-dependent characteristics and the synergism between sigma b and sigma l in listeria monocytogenes egd-e |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7690807/ https://www.ncbi.nlm.nih.gov/pubmed/33114171 http://dx.doi.org/10.3390/microorganisms8111644 |
work_keys_str_mv | AT mattilamirjami transcriptomicandphenotypicanalysesofthesigmabdependentcharacteristicsandthesynergismbetweensigmabandsigmalinlisteriamonocytogenesegde AT somervuopanu transcriptomicandphenotypicanalysesofthesigmabdependentcharacteristicsandthesynergismbetweensigmabandsigmalinlisteriamonocytogenesegde AT korkealahannu transcriptomicandphenotypicanalysesofthesigmabdependentcharacteristicsandthesynergismbetweensigmabandsigmalinlisteriamonocytogenesegde AT stephanroger transcriptomicandphenotypicanalysesofthesigmabdependentcharacteristicsandthesynergismbetweensigmabandsigmalinlisteriamonocytogenesegde AT tasarataurai transcriptomicandphenotypicanalysesofthesigmabdependentcharacteristicsandthesynergismbetweensigmabandsigmalinlisteriamonocytogenesegde |