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Glucosinolate Content in Brassica Genetic Resources and Their Distribution Pattern within and between Inner, Middle, and Outer Leaves

Glucosinolates (GSLs) are sulfur-containing secondary metabolites naturally occurring in Brassica species. The purpose of this study was to identify the GSLs, determine their content, and study their accumulation patterns within and between leaves of kimchi cabbage (Brassica rapa L.) cultivars. GSLs...

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Autores principales: Rhee, Ju-Hee, Choi, Susanna, Lee, Jae-Eun, Hur, On-Sook, Ro, Na-Young, Hwang, Ae-Jin, Ko, Ho-Cheol, Chung, Yun-Jo, Noh, Jae-Jong, Assefa, Awraris Derbie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7690824/
https://www.ncbi.nlm.nih.gov/pubmed/33114129
http://dx.doi.org/10.3390/plants9111421
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author Rhee, Ju-Hee
Choi, Susanna
Lee, Jae-Eun
Hur, On-Sook
Ro, Na-Young
Hwang, Ae-Jin
Ko, Ho-Cheol
Chung, Yun-Jo
Noh, Jae-Jong
Assefa, Awraris Derbie
author_facet Rhee, Ju-Hee
Choi, Susanna
Lee, Jae-Eun
Hur, On-Sook
Ro, Na-Young
Hwang, Ae-Jin
Ko, Ho-Cheol
Chung, Yun-Jo
Noh, Jae-Jong
Assefa, Awraris Derbie
author_sort Rhee, Ju-Hee
collection PubMed
description Glucosinolates (GSLs) are sulfur-containing secondary metabolites naturally occurring in Brassica species. The purpose of this study was to identify the GSLs, determine their content, and study their accumulation patterns within and between leaves of kimchi cabbage (Brassica rapa L.) cultivars. GSLs were analyzed using UPLC-MS/MS in negative electron-spray ionization (ESI(−)) and multiple reaction monitoring (MRM) mode. The total GSL content determined in this study ranged from 621.15 to 42434.21 μmolkg(−1) DW. Aliphatic GSLs predominated, representing from 4.44% to 96.20% of the total GSL content among the entire samples. Glucobrassicanapin (GBN) contributed the greatest proportion while other GSLs such as glucoerucin (ERU) and glucotropaeolin (TRO) were found in relatively low concentrations. Principal component analysis (PCA) yielded three principal components (PCs) with eigenvalues ≥ 1, altogether representing 74.83% of the total variation across the entire dataset. Three kimchi cabbage (S/No. 20, 4, and 2), one leaf mustard (S/No. 26), and one turnip (S/No. 8) genetic resources were well distinguished from other samples. The GSL content varied significantly among the different positions (outer, middle, and inner) of the leaves and sections (top, middle, bottom, green/red, and white) within the leaves. In most of the samples, higher GSL content was observed in the proximal half and white sections and the middle layers of the leaves. GSLs are regarded as allelochemicals; hence, the data related to the patterns of GSLs within the leaf and between leaves at a different position could be useful to understand the defense mechanism of Brassica plants. The observed variability could be useful for breeders to develop Brassica cultivars with high GSL content or specific profiles of GSLs.
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spelling pubmed-76908242020-11-27 Glucosinolate Content in Brassica Genetic Resources and Their Distribution Pattern within and between Inner, Middle, and Outer Leaves Rhee, Ju-Hee Choi, Susanna Lee, Jae-Eun Hur, On-Sook Ro, Na-Young Hwang, Ae-Jin Ko, Ho-Cheol Chung, Yun-Jo Noh, Jae-Jong Assefa, Awraris Derbie Plants (Basel) Article Glucosinolates (GSLs) are sulfur-containing secondary metabolites naturally occurring in Brassica species. The purpose of this study was to identify the GSLs, determine their content, and study their accumulation patterns within and between leaves of kimchi cabbage (Brassica rapa L.) cultivars. GSLs were analyzed using UPLC-MS/MS in negative electron-spray ionization (ESI(−)) and multiple reaction monitoring (MRM) mode. The total GSL content determined in this study ranged from 621.15 to 42434.21 μmolkg(−1) DW. Aliphatic GSLs predominated, representing from 4.44% to 96.20% of the total GSL content among the entire samples. Glucobrassicanapin (GBN) contributed the greatest proportion while other GSLs such as glucoerucin (ERU) and glucotropaeolin (TRO) were found in relatively low concentrations. Principal component analysis (PCA) yielded three principal components (PCs) with eigenvalues ≥ 1, altogether representing 74.83% of the total variation across the entire dataset. Three kimchi cabbage (S/No. 20, 4, and 2), one leaf mustard (S/No. 26), and one turnip (S/No. 8) genetic resources were well distinguished from other samples. The GSL content varied significantly among the different positions (outer, middle, and inner) of the leaves and sections (top, middle, bottom, green/red, and white) within the leaves. In most of the samples, higher GSL content was observed in the proximal half and white sections and the middle layers of the leaves. GSLs are regarded as allelochemicals; hence, the data related to the patterns of GSLs within the leaf and between leaves at a different position could be useful to understand the defense mechanism of Brassica plants. The observed variability could be useful for breeders to develop Brassica cultivars with high GSL content or specific profiles of GSLs. MDPI 2020-10-23 /pmc/articles/PMC7690824/ /pubmed/33114129 http://dx.doi.org/10.3390/plants9111421 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Rhee, Ju-Hee
Choi, Susanna
Lee, Jae-Eun
Hur, On-Sook
Ro, Na-Young
Hwang, Ae-Jin
Ko, Ho-Cheol
Chung, Yun-Jo
Noh, Jae-Jong
Assefa, Awraris Derbie
Glucosinolate Content in Brassica Genetic Resources and Their Distribution Pattern within and between Inner, Middle, and Outer Leaves
title Glucosinolate Content in Brassica Genetic Resources and Their Distribution Pattern within and between Inner, Middle, and Outer Leaves
title_full Glucosinolate Content in Brassica Genetic Resources and Their Distribution Pattern within and between Inner, Middle, and Outer Leaves
title_fullStr Glucosinolate Content in Brassica Genetic Resources and Their Distribution Pattern within and between Inner, Middle, and Outer Leaves
title_full_unstemmed Glucosinolate Content in Brassica Genetic Resources and Their Distribution Pattern within and between Inner, Middle, and Outer Leaves
title_short Glucosinolate Content in Brassica Genetic Resources and Their Distribution Pattern within and between Inner, Middle, and Outer Leaves
title_sort glucosinolate content in brassica genetic resources and their distribution pattern within and between inner, middle, and outer leaves
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7690824/
https://www.ncbi.nlm.nih.gov/pubmed/33114129
http://dx.doi.org/10.3390/plants9111421
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