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Early Season Symptoms on Stem, Inflorescences and Flowers of Grapevine Associated with Botryosphaeriaceae Species

Botryosphaeria dieback caused by several Botryosphaeriaceae species is one of the most important grapevine trunk diseases affecting vineyards worldwide. These fungi cause wedge-shaped perennial cankers and black streaking of the wood and have also been associated with intervein leaf chlorosis, dried...

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Autores principales: Reis, Pedro, Gaspar, Ana, Alves, Artur, Fontaine, Florence, Lourenço, Inês, Saramago, José, Mota, Mariana, Rego, Cecília
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7690898/
https://www.ncbi.nlm.nih.gov/pubmed/33114298
http://dx.doi.org/10.3390/plants9111427
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author Reis, Pedro
Gaspar, Ana
Alves, Artur
Fontaine, Florence
Lourenço, Inês
Saramago, José
Mota, Mariana
Rego, Cecília
author_facet Reis, Pedro
Gaspar, Ana
Alves, Artur
Fontaine, Florence
Lourenço, Inês
Saramago, José
Mota, Mariana
Rego, Cecília
author_sort Reis, Pedro
collection PubMed
description Botryosphaeria dieback caused by several Botryosphaeriaceae species is one of the most important grapevine trunk diseases affecting vineyards worldwide. These fungi cause wedge-shaped perennial cankers and black streaking of the wood and have also been associated with intervein leaf chlorosis, dried or mummified berries, and eventually, the death of the plant. Early season symptoms may sometimes be disregarded by growers, being mistaken with symptoms from other diseases such as downy mildew or botrytis rot. Currently, few studies are available to determine what species may be causing these early season symptoms in grapevines. During the 2018 season, during the flowering period, grapevine samples showing necrosis on green shoots, dried inflorescences, and flowers, were collected in vineyards throughout the central regions of Portugal. Isolations were performed from symptomatic organs, and twenty-three isolates of Botryosphaeriaceae were selected. An analysis of the ITS and part of the translation elongation factor 1-α sequences was performed, revealing that the two main species apparently responsible for these symptoms were Diplodia seriata and Neofusicoccum parvum. In pathogenicity tests conducted on 1-year-old plants grown under controlled conditions in a greenhouse and on field-grown clusters, symptoms were reproduced, confirming the pathogenic behavior of the selection of isolates.
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spelling pubmed-76908982020-11-27 Early Season Symptoms on Stem, Inflorescences and Flowers of Grapevine Associated with Botryosphaeriaceae Species Reis, Pedro Gaspar, Ana Alves, Artur Fontaine, Florence Lourenço, Inês Saramago, José Mota, Mariana Rego, Cecília Plants (Basel) Article Botryosphaeria dieback caused by several Botryosphaeriaceae species is one of the most important grapevine trunk diseases affecting vineyards worldwide. These fungi cause wedge-shaped perennial cankers and black streaking of the wood and have also been associated with intervein leaf chlorosis, dried or mummified berries, and eventually, the death of the plant. Early season symptoms may sometimes be disregarded by growers, being mistaken with symptoms from other diseases such as downy mildew or botrytis rot. Currently, few studies are available to determine what species may be causing these early season symptoms in grapevines. During the 2018 season, during the flowering period, grapevine samples showing necrosis on green shoots, dried inflorescences, and flowers, were collected in vineyards throughout the central regions of Portugal. Isolations were performed from symptomatic organs, and twenty-three isolates of Botryosphaeriaceae were selected. An analysis of the ITS and part of the translation elongation factor 1-α sequences was performed, revealing that the two main species apparently responsible for these symptoms were Diplodia seriata and Neofusicoccum parvum. In pathogenicity tests conducted on 1-year-old plants grown under controlled conditions in a greenhouse and on field-grown clusters, symptoms were reproduced, confirming the pathogenic behavior of the selection of isolates. MDPI 2020-10-24 /pmc/articles/PMC7690898/ /pubmed/33114298 http://dx.doi.org/10.3390/plants9111427 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Reis, Pedro
Gaspar, Ana
Alves, Artur
Fontaine, Florence
Lourenço, Inês
Saramago, José
Mota, Mariana
Rego, Cecília
Early Season Symptoms on Stem, Inflorescences and Flowers of Grapevine Associated with Botryosphaeriaceae Species
title Early Season Symptoms on Stem, Inflorescences and Flowers of Grapevine Associated with Botryosphaeriaceae Species
title_full Early Season Symptoms on Stem, Inflorescences and Flowers of Grapevine Associated with Botryosphaeriaceae Species
title_fullStr Early Season Symptoms on Stem, Inflorescences and Flowers of Grapevine Associated with Botryosphaeriaceae Species
title_full_unstemmed Early Season Symptoms on Stem, Inflorescences and Flowers of Grapevine Associated with Botryosphaeriaceae Species
title_short Early Season Symptoms on Stem, Inflorescences and Flowers of Grapevine Associated with Botryosphaeriaceae Species
title_sort early season symptoms on stem, inflorescences and flowers of grapevine associated with botryosphaeriaceae species
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7690898/
https://www.ncbi.nlm.nih.gov/pubmed/33114298
http://dx.doi.org/10.3390/plants9111427
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