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Mechanism of action of Panax notoginoside against lung cancer in mice based on response to CTSB gene

BACKGROUND: This study aimed to investigate the mechanism of action of Panax notoginoside (PNS) against lung cancer and inhibition of lung cancer cell proliferation by the drug at different concentrations in a mouse model, considering the cathepsin B (CTSB) gene as a target. METHODS: The mice were r...

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Autores principales: Zhang, Jizhou, Zhou, Bin, Jin, Song, Huang, Zhiyou, Ma, Bidong, Shao, Qiqi, Zhu, Wenzong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7691060/
https://www.ncbi.nlm.nih.gov/pubmed/33238959
http://dx.doi.org/10.1186/s12906-020-03159-0
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author Zhang, Jizhou
Zhou, Bin
Jin, Song
Huang, Zhiyou
Ma, Bidong
Shao, Qiqi
Zhu, Wenzong
author_facet Zhang, Jizhou
Zhou, Bin
Jin, Song
Huang, Zhiyou
Ma, Bidong
Shao, Qiqi
Zhu, Wenzong
author_sort Zhang, Jizhou
collection PubMed
description BACKGROUND: This study aimed to investigate the mechanism of action of Panax notoginoside (PNS) against lung cancer and inhibition of lung cancer cell proliferation by the drug at different concentrations in a mouse model, considering the cathepsin B (CTSB) gene as a target. METHODS: The mice were randomly assigned into the following five groups: normal control, tumor-bearing, low-dose Panax notoginoside (TSPN), medium-dose TSPN, and high-dose TSPN. All mice were treated with physiological saline or TSPN at different concentrations for 28 days consecutively by gavage. The tumor size was measured, the tumor growth was observed, and the survival curve was drawn. At different time points, the expression of the CTSB gene was detected using quantitative fluorescent polymerase chain reaction, Western blot analysis, and indirect immunofluorescence. The serum indices, such as carcinoembryonic antigen (CEA), neuron-specific enolase (NSE), and Soluble fragment of cytokeratin 19 (CYFRA21), were detected by enzyme-linked immunosorbent assay. RESULTS: In vivo, PNS could directly inhibit the expression of the CTSB gene in tumors of mice, limit tumor growth, and alter tumor-related indices, such as CEA, NSE, and CYFRA21 levels, in the serum to different extents simultaneously. CONCLUSION: CTSB gene was closely related to the pathogenesis of lung cancer. PNS could act on the CTSB gene, downregulate the expression of CTSB in lung cancer cells, inhibit the proliferation and invasion of tumors, and prolong the survival period. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12906-020-03159-0.
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spelling pubmed-76910602020-11-30 Mechanism of action of Panax notoginoside against lung cancer in mice based on response to CTSB gene Zhang, Jizhou Zhou, Bin Jin, Song Huang, Zhiyou Ma, Bidong Shao, Qiqi Zhu, Wenzong BMC Complement Med Ther Research Article BACKGROUND: This study aimed to investigate the mechanism of action of Panax notoginoside (PNS) against lung cancer and inhibition of lung cancer cell proliferation by the drug at different concentrations in a mouse model, considering the cathepsin B (CTSB) gene as a target. METHODS: The mice were randomly assigned into the following five groups: normal control, tumor-bearing, low-dose Panax notoginoside (TSPN), medium-dose TSPN, and high-dose TSPN. All mice were treated with physiological saline or TSPN at different concentrations for 28 days consecutively by gavage. The tumor size was measured, the tumor growth was observed, and the survival curve was drawn. At different time points, the expression of the CTSB gene was detected using quantitative fluorescent polymerase chain reaction, Western blot analysis, and indirect immunofluorescence. The serum indices, such as carcinoembryonic antigen (CEA), neuron-specific enolase (NSE), and Soluble fragment of cytokeratin 19 (CYFRA21), were detected by enzyme-linked immunosorbent assay. RESULTS: In vivo, PNS could directly inhibit the expression of the CTSB gene in tumors of mice, limit tumor growth, and alter tumor-related indices, such as CEA, NSE, and CYFRA21 levels, in the serum to different extents simultaneously. CONCLUSION: CTSB gene was closely related to the pathogenesis of lung cancer. PNS could act on the CTSB gene, downregulate the expression of CTSB in lung cancer cells, inhibit the proliferation and invasion of tumors, and prolong the survival period. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12906-020-03159-0. BioMed Central 2020-11-25 /pmc/articles/PMC7691060/ /pubmed/33238959 http://dx.doi.org/10.1186/s12906-020-03159-0 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Zhang, Jizhou
Zhou, Bin
Jin, Song
Huang, Zhiyou
Ma, Bidong
Shao, Qiqi
Zhu, Wenzong
Mechanism of action of Panax notoginoside against lung cancer in mice based on response to CTSB gene
title Mechanism of action of Panax notoginoside against lung cancer in mice based on response to CTSB gene
title_full Mechanism of action of Panax notoginoside against lung cancer in mice based on response to CTSB gene
title_fullStr Mechanism of action of Panax notoginoside against lung cancer in mice based on response to CTSB gene
title_full_unstemmed Mechanism of action of Panax notoginoside against lung cancer in mice based on response to CTSB gene
title_short Mechanism of action of Panax notoginoside against lung cancer in mice based on response to CTSB gene
title_sort mechanism of action of panax notoginoside against lung cancer in mice based on response to ctsb gene
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7691060/
https://www.ncbi.nlm.nih.gov/pubmed/33238959
http://dx.doi.org/10.1186/s12906-020-03159-0
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