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Resistotyping and extended-spectrum beta-lactamase genes among Escherichia coli from wastewater treatment plants and recipient surface water for reuse in South Africa

The spread of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli has increased in different environments. This study aimed to evaluate the patterns of antibiotic resistance and ESBL genes among E. coli isolates collected from wastewater and recipient surface water in South Africa. Fifte...

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Autores principales: Nzima, B., Adegoke, A.A., Ofon, U.A., Al-Dahmoshi, H.O.M., Saki, M., Ndubuisi-Nnaji, U.U., Inyang, C.U.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7691180/
https://www.ncbi.nlm.nih.gov/pubmed/33294195
http://dx.doi.org/10.1016/j.nmni.2020.100803
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author Nzima, B.
Adegoke, A.A.
Ofon, U.A.
Al-Dahmoshi, H.O.M.
Saki, M.
Ndubuisi-Nnaji, U.U.
Inyang, C.U.
author_facet Nzima, B.
Adegoke, A.A.
Ofon, U.A.
Al-Dahmoshi, H.O.M.
Saki, M.
Ndubuisi-Nnaji, U.U.
Inyang, C.U.
author_sort Nzima, B.
collection PubMed
description The spread of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli has increased in different environments. This study aimed to evaluate the patterns of antibiotic resistance and ESBL genes among E. coli isolates collected from wastewater and recipient surface water in South Africa. Fifteen samples containing nine wastewater and six river water samples were collected from a local wastewater treatment plant. The E. coli isolates were detected using standard microbiology methods. Antibiotic susceptibility testing was performed using disc diffusion agar. The occurrence of bla(CTX-M), bla(SHV) and bla(TEM) ESBL genes was investigated by PCR. Exactly 140 isolates were selected from the primary enumeration plates with a log(10) CFU/mL count that ranged from 4.1 to 4.2 (influent), 4.2 to 4.5 (biofilter) and 2.5 to 3.3 (effluent). The wastewater effluent showed an impact on the receiving water environment, as the treatment efficiency was 92% and the downstream log(10) CFU/mL count (range, 3.6–3.8 log(10) CFU/mL) was higher than the upstream count (range, 3.3–3.6 log(10) CFU/mL). Antibiotic testing results showed that 40% to 100% of E. coli isolates were resistant to ampicillin, penicillin, tetracycline and cefotaxime but susceptible to imipenem, meropenem and ciprofloxacin. A total of 40 studied isolates (28.6%) had both the bla(TEM) and bla(CTX-M) genes, while no bla(SHV) was detected. The wastewater treatment plants contributed multidrug-resistant ESBL-producing E. coli isolates that can be potential environmental health risks. Regular monitoring policies are recommended to prevent the spread of antibiotic resistance in the region.
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spelling pubmed-76911802020-12-07 Resistotyping and extended-spectrum beta-lactamase genes among Escherichia coli from wastewater treatment plants and recipient surface water for reuse in South Africa Nzima, B. Adegoke, A.A. Ofon, U.A. Al-Dahmoshi, H.O.M. Saki, M. Ndubuisi-Nnaji, U.U. Inyang, C.U. New Microbes New Infect Original Article The spread of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli has increased in different environments. This study aimed to evaluate the patterns of antibiotic resistance and ESBL genes among E. coli isolates collected from wastewater and recipient surface water in South Africa. Fifteen samples containing nine wastewater and six river water samples were collected from a local wastewater treatment plant. The E. coli isolates were detected using standard microbiology methods. Antibiotic susceptibility testing was performed using disc diffusion agar. The occurrence of bla(CTX-M), bla(SHV) and bla(TEM) ESBL genes was investigated by PCR. Exactly 140 isolates were selected from the primary enumeration plates with a log(10) CFU/mL count that ranged from 4.1 to 4.2 (influent), 4.2 to 4.5 (biofilter) and 2.5 to 3.3 (effluent). The wastewater effluent showed an impact on the receiving water environment, as the treatment efficiency was 92% and the downstream log(10) CFU/mL count (range, 3.6–3.8 log(10) CFU/mL) was higher than the upstream count (range, 3.3–3.6 log(10) CFU/mL). Antibiotic testing results showed that 40% to 100% of E. coli isolates were resistant to ampicillin, penicillin, tetracycline and cefotaxime but susceptible to imipenem, meropenem and ciprofloxacin. A total of 40 studied isolates (28.6%) had both the bla(TEM) and bla(CTX-M) genes, while no bla(SHV) was detected. The wastewater treatment plants contributed multidrug-resistant ESBL-producing E. coli isolates that can be potential environmental health risks. Regular monitoring policies are recommended to prevent the spread of antibiotic resistance in the region. Elsevier 2020-10-31 /pmc/articles/PMC7691180/ /pubmed/33294195 http://dx.doi.org/10.1016/j.nmni.2020.100803 Text en © 2020 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Nzima, B.
Adegoke, A.A.
Ofon, U.A.
Al-Dahmoshi, H.O.M.
Saki, M.
Ndubuisi-Nnaji, U.U.
Inyang, C.U.
Resistotyping and extended-spectrum beta-lactamase genes among Escherichia coli from wastewater treatment plants and recipient surface water for reuse in South Africa
title Resistotyping and extended-spectrum beta-lactamase genes among Escherichia coli from wastewater treatment plants and recipient surface water for reuse in South Africa
title_full Resistotyping and extended-spectrum beta-lactamase genes among Escherichia coli from wastewater treatment plants and recipient surface water for reuse in South Africa
title_fullStr Resistotyping and extended-spectrum beta-lactamase genes among Escherichia coli from wastewater treatment plants and recipient surface water for reuse in South Africa
title_full_unstemmed Resistotyping and extended-spectrum beta-lactamase genes among Escherichia coli from wastewater treatment plants and recipient surface water for reuse in South Africa
title_short Resistotyping and extended-spectrum beta-lactamase genes among Escherichia coli from wastewater treatment plants and recipient surface water for reuse in South Africa
title_sort resistotyping and extended-spectrum beta-lactamase genes among escherichia coli from wastewater treatment plants and recipient surface water for reuse in south africa
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7691180/
https://www.ncbi.nlm.nih.gov/pubmed/33294195
http://dx.doi.org/10.1016/j.nmni.2020.100803
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