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Effects of surface sub-micrometer topography following oxalic acid treatment on bone quantity and quality around dental implants in rabbit tibiae

BACKGROUND: To explore the effects of topographical modification of titanium substrates at submicron level by oxalic acid treatment on bone quality and quantity around dental implants in rabbit tibiae. METHODS: A total of 60 blasted CP-grade IV titanium dental implants were used. Twenty-eight contro...

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Autores principales: Kanai, Riho, Kuroshima, Shinichiro, Kamo, Michimasa, Sasaki, Muneteru, Uto, Yusuke, Inaba, Nao, Uchida, Yusuke, Hayano, Hiroki, Tamaki, Saki, Inoue, Maaya, Sawase, Takashi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7691415/
https://www.ncbi.nlm.nih.gov/pubmed/33244653
http://dx.doi.org/10.1186/s40729-020-00275-x
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author Kanai, Riho
Kuroshima, Shinichiro
Kamo, Michimasa
Sasaki, Muneteru
Uto, Yusuke
Inaba, Nao
Uchida, Yusuke
Hayano, Hiroki
Tamaki, Saki
Inoue, Maaya
Sawase, Takashi
author_facet Kanai, Riho
Kuroshima, Shinichiro
Kamo, Michimasa
Sasaki, Muneteru
Uto, Yusuke
Inaba, Nao
Uchida, Yusuke
Hayano, Hiroki
Tamaki, Saki
Inoue, Maaya
Sawase, Takashi
author_sort Kanai, Riho
collection PubMed
description BACKGROUND: To explore the effects of topographical modification of titanium substrates at submicron level by oxalic acid treatment on bone quality and quantity around dental implants in rabbit tibiae. METHODS: A total of 60 blasted CP-grade IV titanium dental implants were used. Twenty-eight control implant surfaces were treated with a mixture of HCl/H(2)SO(4), whereas 28 other test implant surfaces were treated with oxalic acid following HCl/H(2)SO(4) treatment. Two randomly selected sets of control or test implants were placed in randomly selected proximal tibiae of 14 female Japanese white rabbits. Euthanasia was performed 4 and 8 weeks post-implant placement. Bone to implant contact (BIC), bone area fraction (BAF), ratios of mature and immature bone to total bone, and the amount and types of collagen fibers were evaluated quantitatively. Two control and two test implants were used to analyze surface characteristics. RESULTS: Treatment by oxalic acid significantly decreased Sa and increased Ra of test implant surfaces. BIC in test implants was increased without alteration of BAF and collagen contents at 4 and 8 weeks after implant placement when compared with control implants. The ratios of immature and mature bone to total bone differed significantly between groups at 4 weeks post-implantation. Treatment by oxalic acid increased type I collagen and decreased type III collagen in bone matrices around test implants when compared with control implants at 8 weeks after implant placement. The effects of topographical changes of implant surfaces induced by oxalic acid on BAF, mature bone, collagen contents, and type I collagen were significantly promoted with decreased immature bone formation and type III collagen in the later 4 weeks post-implantation. CONCLUSIONS: Treatment of implant surfaces with oxalic acid rapidly increases osseointegration from the early stages after implantation. Moreover, submicron topographical changes of dental implants induced by oxalic acid improve bone quality based on bone maturation and increased production of type I collagen surrounding dental implants in the late stage after implant placement. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s40729-020-00275-x.
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spelling pubmed-76914152020-11-30 Effects of surface sub-micrometer topography following oxalic acid treatment on bone quantity and quality around dental implants in rabbit tibiae Kanai, Riho Kuroshima, Shinichiro Kamo, Michimasa Sasaki, Muneteru Uto, Yusuke Inaba, Nao Uchida, Yusuke Hayano, Hiroki Tamaki, Saki Inoue, Maaya Sawase, Takashi Int J Implant Dent Research BACKGROUND: To explore the effects of topographical modification of titanium substrates at submicron level by oxalic acid treatment on bone quality and quantity around dental implants in rabbit tibiae. METHODS: A total of 60 blasted CP-grade IV titanium dental implants were used. Twenty-eight control implant surfaces were treated with a mixture of HCl/H(2)SO(4), whereas 28 other test implant surfaces were treated with oxalic acid following HCl/H(2)SO(4) treatment. Two randomly selected sets of control or test implants were placed in randomly selected proximal tibiae of 14 female Japanese white rabbits. Euthanasia was performed 4 and 8 weeks post-implant placement. Bone to implant contact (BIC), bone area fraction (BAF), ratios of mature and immature bone to total bone, and the amount and types of collagen fibers were evaluated quantitatively. Two control and two test implants were used to analyze surface characteristics. RESULTS: Treatment by oxalic acid significantly decreased Sa and increased Ra of test implant surfaces. BIC in test implants was increased without alteration of BAF and collagen contents at 4 and 8 weeks after implant placement when compared with control implants. The ratios of immature and mature bone to total bone differed significantly between groups at 4 weeks post-implantation. Treatment by oxalic acid increased type I collagen and decreased type III collagen in bone matrices around test implants when compared with control implants at 8 weeks after implant placement. The effects of topographical changes of implant surfaces induced by oxalic acid on BAF, mature bone, collagen contents, and type I collagen were significantly promoted with decreased immature bone formation and type III collagen in the later 4 weeks post-implantation. CONCLUSIONS: Treatment of implant surfaces with oxalic acid rapidly increases osseointegration from the early stages after implantation. Moreover, submicron topographical changes of dental implants induced by oxalic acid improve bone quality based on bone maturation and increased production of type I collagen surrounding dental implants in the late stage after implant placement. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s40729-020-00275-x. Springer Berlin Heidelberg 2020-11-27 /pmc/articles/PMC7691415/ /pubmed/33244653 http://dx.doi.org/10.1186/s40729-020-00275-x Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Research
Kanai, Riho
Kuroshima, Shinichiro
Kamo, Michimasa
Sasaki, Muneteru
Uto, Yusuke
Inaba, Nao
Uchida, Yusuke
Hayano, Hiroki
Tamaki, Saki
Inoue, Maaya
Sawase, Takashi
Effects of surface sub-micrometer topography following oxalic acid treatment on bone quantity and quality around dental implants in rabbit tibiae
title Effects of surface sub-micrometer topography following oxalic acid treatment on bone quantity and quality around dental implants in rabbit tibiae
title_full Effects of surface sub-micrometer topography following oxalic acid treatment on bone quantity and quality around dental implants in rabbit tibiae
title_fullStr Effects of surface sub-micrometer topography following oxalic acid treatment on bone quantity and quality around dental implants in rabbit tibiae
title_full_unstemmed Effects of surface sub-micrometer topography following oxalic acid treatment on bone quantity and quality around dental implants in rabbit tibiae
title_short Effects of surface sub-micrometer topography following oxalic acid treatment on bone quantity and quality around dental implants in rabbit tibiae
title_sort effects of surface sub-micrometer topography following oxalic acid treatment on bone quantity and quality around dental implants in rabbit tibiae
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7691415/
https://www.ncbi.nlm.nih.gov/pubmed/33244653
http://dx.doi.org/10.1186/s40729-020-00275-x
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