Cargando…
Pre-analytical Practices in the Molecular Diagnostic Tests, A Concise Review
Molecular assays for detection of nucleic acids in biologic specimens are valuable diagnostic tools supporting clinical diagnoses and therapeutic decisions. Pre-analytical errors, which occur before or during processing of nucleic acid extraction, contribute a significant role in common errors that...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Iranian Society of Pathology
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7691716/ https://www.ncbi.nlm.nih.gov/pubmed/33391375 http://dx.doi.org/10.30699/ijp.2020.124315.2357 |
_version_ | 1783614353213227008 |
---|---|
author | Sotoudeh Anvari, Maryam Gharib, Atoosa Abolhasani, Maryam Azari-Yam, Aileen Hossieni Gharalari, Farzaneh Safavi, Moeinadin Zare Mirzaie, Ali Vasei, Mohammad |
author_facet | Sotoudeh Anvari, Maryam Gharib, Atoosa Abolhasani, Maryam Azari-Yam, Aileen Hossieni Gharalari, Farzaneh Safavi, Moeinadin Zare Mirzaie, Ali Vasei, Mohammad |
author_sort | Sotoudeh Anvari, Maryam |
collection | PubMed |
description | Molecular assays for detection of nucleic acids in biologic specimens are valuable diagnostic tools supporting clinical diagnoses and therapeutic decisions. Pre-analytical errors, which occur before or during processing of nucleic acid extraction, contribute a significant role in common errors that take place in molecular laboratories. Certain practices in specimen collection, transportation, and storage can affect the integrity of nucleic acids before analysis. Applying best practices in these steps, helps to minimize those errors and leads to better decisions in patient diagnosis and treatment. Widely acceptable recommendations, which are for optimal molecular assays associated with pre-analytic variables, are limited. In this article, we have reviewed most of the important issues in sample handling from bed to bench before starting molecular tests, which can be used in diagnostic as well as research laboratories. We have addressed the most important pre-analytical points in performing molecular analysis in fixed and unfixed solid tissues, whole blood, serum, plasma, as well as most of the body fluids including urine, fecal and bronchial samples, as well as prenatal diagnosis samples. |
format | Online Article Text |
id | pubmed-7691716 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Iranian Society of Pathology |
record_format | MEDLINE/PubMed |
spelling | pubmed-76917162021-01-01 Pre-analytical Practices in the Molecular Diagnostic Tests, A Concise Review Sotoudeh Anvari, Maryam Gharib, Atoosa Abolhasani, Maryam Azari-Yam, Aileen Hossieni Gharalari, Farzaneh Safavi, Moeinadin Zare Mirzaie, Ali Vasei, Mohammad Iran J Pathol Review Article Molecular assays for detection of nucleic acids in biologic specimens are valuable diagnostic tools supporting clinical diagnoses and therapeutic decisions. Pre-analytical errors, which occur before or during processing of nucleic acid extraction, contribute a significant role in common errors that take place in molecular laboratories. Certain practices in specimen collection, transportation, and storage can affect the integrity of nucleic acids before analysis. Applying best practices in these steps, helps to minimize those errors and leads to better decisions in patient diagnosis and treatment. Widely acceptable recommendations, which are for optimal molecular assays associated with pre-analytic variables, are limited. In this article, we have reviewed most of the important issues in sample handling from bed to bench before starting molecular tests, which can be used in diagnostic as well as research laboratories. We have addressed the most important pre-analytical points in performing molecular analysis in fixed and unfixed solid tissues, whole blood, serum, plasma, as well as most of the body fluids including urine, fecal and bronchial samples, as well as prenatal diagnosis samples. Iranian Society of Pathology 2021 2020-11-10 /pmc/articles/PMC7691716/ /pubmed/33391375 http://dx.doi.org/10.30699/ijp.2020.124315.2357 Text en This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Review Article Sotoudeh Anvari, Maryam Gharib, Atoosa Abolhasani, Maryam Azari-Yam, Aileen Hossieni Gharalari, Farzaneh Safavi, Moeinadin Zare Mirzaie, Ali Vasei, Mohammad Pre-analytical Practices in the Molecular Diagnostic Tests, A Concise Review |
title | Pre-analytical Practices in the Molecular Diagnostic Tests, A Concise Review |
title_full | Pre-analytical Practices in the Molecular Diagnostic Tests, A Concise Review |
title_fullStr | Pre-analytical Practices in the Molecular Diagnostic Tests, A Concise Review |
title_full_unstemmed | Pre-analytical Practices in the Molecular Diagnostic Tests, A Concise Review |
title_short | Pre-analytical Practices in the Molecular Diagnostic Tests, A Concise Review |
title_sort | pre-analytical practices in the molecular diagnostic tests, a concise review |
topic | Review Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7691716/ https://www.ncbi.nlm.nih.gov/pubmed/33391375 http://dx.doi.org/10.30699/ijp.2020.124315.2357 |
work_keys_str_mv | AT sotoudehanvarimaryam preanalyticalpracticesinthemoleculardiagnostictestsaconcisereview AT gharibatoosa preanalyticalpracticesinthemoleculardiagnostictestsaconcisereview AT abolhasanimaryam preanalyticalpracticesinthemoleculardiagnostictestsaconcisereview AT azariyamaileen preanalyticalpracticesinthemoleculardiagnostictestsaconcisereview AT hossienigharalarifarzaneh preanalyticalpracticesinthemoleculardiagnostictestsaconcisereview AT safavimoeinadin preanalyticalpracticesinthemoleculardiagnostictestsaconcisereview AT zaremirzaieali preanalyticalpracticesinthemoleculardiagnostictestsaconcisereview AT vaseimohammad preanalyticalpracticesinthemoleculardiagnostictestsaconcisereview |