Species-level identification of trypanosomes infecting Australian wildlife by High-Resolution Melting - Real Time Quantitative Polymerase Chain Reaction (HRM-qPCR)

Conventional nested PCR and Sanger sequencing methods are currently the gold standards for detecting trypanosomes in wildlife. However, these techniques are time-consuming and can often overlook mixed infections. True trypanosome prevalence can thus be underrepresented. Here, we designed an 18S rDNA...

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Detalles Bibliográficos
Autores principales: Keatley, S., Botero, A., Fosu-Nyarko, J., Pallant, L., Northover, A., Thompson, R.C.A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7691731/
https://www.ncbi.nlm.nih.gov/pubmed/33294365
http://dx.doi.org/10.1016/j.ijppaw.2020.11.003

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