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Development of Surface-Enhanced Raman Scattering (SERS)-Based Surface-Corrugated Nanopillars for Biomolecular Detection of Colorectal Cancer

In this paper, a nanobiosensor with surface-enhanced Raman scattering (SERS) capability is introduced for highly sensitive miRNA detection in colorectal cancer. This sensor was designed and fabricated by employing a nanoshielding mechanism from nanopolystyrene beads to resist reactive ion etching an...

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Autores principales: Chen, Kuan-Hung, Pan, Meng-Ju, Jargalsaikhan, Zoljargal, Ishdorj, Tseren-Onolt, Tseng, Fan-Gang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7692079/
https://www.ncbi.nlm.nih.gov/pubmed/33142781
http://dx.doi.org/10.3390/bios10110163
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author Chen, Kuan-Hung
Pan, Meng-Ju
Jargalsaikhan, Zoljargal
Ishdorj, Tseren-Onolt
Tseng, Fan-Gang
author_facet Chen, Kuan-Hung
Pan, Meng-Ju
Jargalsaikhan, Zoljargal
Ishdorj, Tseren-Onolt
Tseng, Fan-Gang
author_sort Chen, Kuan-Hung
collection PubMed
description In this paper, a nanobiosensor with surface-enhanced Raman scattering (SERS) capability is introduced for highly sensitive miRNA detection in colorectal cancer. This sensor was designed and fabricated by employing a nanoshielding mechanism from nanopolystyrene beads to resist reactive ion etching and allow anisotropic electrochemical etching, producing high-aspect-ratio, surface-corrugated nanopillars (SiNPs) on a silicon wafer to create extensive hot spots along the nanopillars for improved SERS signals. SERS enhancements were correlated with nanorange roughness, indicating that hot spots along the pillars were the crucial factor to improve the SERS effect. We achieved the detection capability of a trace amount of R6G (10(−8) M), and the SERS signal enhancement factor (EF) was close to 1.0 × 10(7) on surface-corrugated gold SiNPs. miRNA samples were also demonstrated on this sensor with good sensitivity and specificity. The target molecule miR-21-Cy5 was easily monitored through Raman spectrum variation with a PCR-comparable concentration at around 100 pM with clear nucleotide-specific Raman signals, which is also suitable for biomolecule sensing.
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spelling pubmed-76920792020-11-28 Development of Surface-Enhanced Raman Scattering (SERS)-Based Surface-Corrugated Nanopillars for Biomolecular Detection of Colorectal Cancer Chen, Kuan-Hung Pan, Meng-Ju Jargalsaikhan, Zoljargal Ishdorj, Tseren-Onolt Tseng, Fan-Gang Biosensors (Basel) Article In this paper, a nanobiosensor with surface-enhanced Raman scattering (SERS) capability is introduced for highly sensitive miRNA detection in colorectal cancer. This sensor was designed and fabricated by employing a nanoshielding mechanism from nanopolystyrene beads to resist reactive ion etching and allow anisotropic electrochemical etching, producing high-aspect-ratio, surface-corrugated nanopillars (SiNPs) on a silicon wafer to create extensive hot spots along the nanopillars for improved SERS signals. SERS enhancements were correlated with nanorange roughness, indicating that hot spots along the pillars were the crucial factor to improve the SERS effect. We achieved the detection capability of a trace amount of R6G (10(−8) M), and the SERS signal enhancement factor (EF) was close to 1.0 × 10(7) on surface-corrugated gold SiNPs. miRNA samples were also demonstrated on this sensor with good sensitivity and specificity. The target molecule miR-21-Cy5 was easily monitored through Raman spectrum variation with a PCR-comparable concentration at around 100 pM with clear nucleotide-specific Raman signals, which is also suitable for biomolecule sensing. MDPI 2020-10-31 /pmc/articles/PMC7692079/ /pubmed/33142781 http://dx.doi.org/10.3390/bios10110163 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chen, Kuan-Hung
Pan, Meng-Ju
Jargalsaikhan, Zoljargal
Ishdorj, Tseren-Onolt
Tseng, Fan-Gang
Development of Surface-Enhanced Raman Scattering (SERS)-Based Surface-Corrugated Nanopillars for Biomolecular Detection of Colorectal Cancer
title Development of Surface-Enhanced Raman Scattering (SERS)-Based Surface-Corrugated Nanopillars for Biomolecular Detection of Colorectal Cancer
title_full Development of Surface-Enhanced Raman Scattering (SERS)-Based Surface-Corrugated Nanopillars for Biomolecular Detection of Colorectal Cancer
title_fullStr Development of Surface-Enhanced Raman Scattering (SERS)-Based Surface-Corrugated Nanopillars for Biomolecular Detection of Colorectal Cancer
title_full_unstemmed Development of Surface-Enhanced Raman Scattering (SERS)-Based Surface-Corrugated Nanopillars for Biomolecular Detection of Colorectal Cancer
title_short Development of Surface-Enhanced Raman Scattering (SERS)-Based Surface-Corrugated Nanopillars for Biomolecular Detection of Colorectal Cancer
title_sort development of surface-enhanced raman scattering (sers)-based surface-corrugated nanopillars for biomolecular detection of colorectal cancer
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7692079/
https://www.ncbi.nlm.nih.gov/pubmed/33142781
http://dx.doi.org/10.3390/bios10110163
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