Cargando…
Developing a Low-Cost, Simple-to-Use Electrochemical Sensor for the Detection of Circulating Tumour DNA in Human Fluids
It is well-known that two major issues, preventing improved outcomes from cancer are late diagnosis and the evolution of drug resistance during chemotherapy, therefore technologies that address these issues can have a transformative effect on healthcare workflows. In this work we present a simple, l...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7692145/ https://www.ncbi.nlm.nih.gov/pubmed/33126531 http://dx.doi.org/10.3390/bios10110156 |
_version_ | 1783614443541757952 |
---|---|
author | Attoye, Bukola Pou, Chantevy Blair, Ewen Rinaldi, Christopher Thomson, Fiona Baker, Matthew J. Corrigan, Damion K. |
author_facet | Attoye, Bukola Pou, Chantevy Blair, Ewen Rinaldi, Christopher Thomson, Fiona Baker, Matthew J. Corrigan, Damion K. |
author_sort | Attoye, Bukola |
collection | PubMed |
description | It is well-known that two major issues, preventing improved outcomes from cancer are late diagnosis and the evolution of drug resistance during chemotherapy, therefore technologies that address these issues can have a transformative effect on healthcare workflows. In this work we present a simple, low-cost DNA biosensor that was developed specifically to detect mutations in a key oncogene (KRAS). The sensor employed was a screen-printed array of carbon electrodes, used to perform parallel measurements of DNA hybridisation. A DNA amplification reaction was developed with primers for mutant and wild type KRAS sequences which amplified target sequences from representative clinical samples to detectable levels in as few as twenty cycles. High levels of sensitivity were demonstrated alongside a clear exemplar of assay specificity by showing the mutant KRAS sequence was detectable against a significant background of wild type DNA following amplification and hybridisation on the sensor surface. The time to result was found to be 3.5 h with considerable potential for optimisation through assay integration. This quick and versatile biosensor has the potential to be deployed in a low-cost, point-of-care test where patients can be screened either for early diagnosis purposes or monitoring of response to therapy. |
format | Online Article Text |
id | pubmed-7692145 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-76921452020-11-28 Developing a Low-Cost, Simple-to-Use Electrochemical Sensor for the Detection of Circulating Tumour DNA in Human Fluids Attoye, Bukola Pou, Chantevy Blair, Ewen Rinaldi, Christopher Thomson, Fiona Baker, Matthew J. Corrigan, Damion K. Biosensors (Basel) Article It is well-known that two major issues, preventing improved outcomes from cancer are late diagnosis and the evolution of drug resistance during chemotherapy, therefore technologies that address these issues can have a transformative effect on healthcare workflows. In this work we present a simple, low-cost DNA biosensor that was developed specifically to detect mutations in a key oncogene (KRAS). The sensor employed was a screen-printed array of carbon electrodes, used to perform parallel measurements of DNA hybridisation. A DNA amplification reaction was developed with primers for mutant and wild type KRAS sequences which amplified target sequences from representative clinical samples to detectable levels in as few as twenty cycles. High levels of sensitivity were demonstrated alongside a clear exemplar of assay specificity by showing the mutant KRAS sequence was detectable against a significant background of wild type DNA following amplification and hybridisation on the sensor surface. The time to result was found to be 3.5 h with considerable potential for optimisation through assay integration. This quick and versatile biosensor has the potential to be deployed in a low-cost, point-of-care test where patients can be screened either for early diagnosis purposes or monitoring of response to therapy. MDPI 2020-10-28 /pmc/articles/PMC7692145/ /pubmed/33126531 http://dx.doi.org/10.3390/bios10110156 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Attoye, Bukola Pou, Chantevy Blair, Ewen Rinaldi, Christopher Thomson, Fiona Baker, Matthew J. Corrigan, Damion K. Developing a Low-Cost, Simple-to-Use Electrochemical Sensor for the Detection of Circulating Tumour DNA in Human Fluids |
title | Developing a Low-Cost, Simple-to-Use Electrochemical Sensor for the Detection of Circulating Tumour DNA in Human Fluids |
title_full | Developing a Low-Cost, Simple-to-Use Electrochemical Sensor for the Detection of Circulating Tumour DNA in Human Fluids |
title_fullStr | Developing a Low-Cost, Simple-to-Use Electrochemical Sensor for the Detection of Circulating Tumour DNA in Human Fluids |
title_full_unstemmed | Developing a Low-Cost, Simple-to-Use Electrochemical Sensor for the Detection of Circulating Tumour DNA in Human Fluids |
title_short | Developing a Low-Cost, Simple-to-Use Electrochemical Sensor for the Detection of Circulating Tumour DNA in Human Fluids |
title_sort | developing a low-cost, simple-to-use electrochemical sensor for the detection of circulating tumour dna in human fluids |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7692145/ https://www.ncbi.nlm.nih.gov/pubmed/33126531 http://dx.doi.org/10.3390/bios10110156 |
work_keys_str_mv | AT attoyebukola developingalowcostsimpletouseelectrochemicalsensorforthedetectionofcirculatingtumourdnainhumanfluids AT pouchantevy developingalowcostsimpletouseelectrochemicalsensorforthedetectionofcirculatingtumourdnainhumanfluids AT blairewen developingalowcostsimpletouseelectrochemicalsensorforthedetectionofcirculatingtumourdnainhumanfluids AT rinaldichristopher developingalowcostsimpletouseelectrochemicalsensorforthedetectionofcirculatingtumourdnainhumanfluids AT thomsonfiona developingalowcostsimpletouseelectrochemicalsensorforthedetectionofcirculatingtumourdnainhumanfluids AT bakermatthewj developingalowcostsimpletouseelectrochemicalsensorforthedetectionofcirculatingtumourdnainhumanfluids AT corrigandamionk developingalowcostsimpletouseelectrochemicalsensorforthedetectionofcirculatingtumourdnainhumanfluids |