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Biopolymer Hydrogel Scaffold as an Artificial Cell Niche for Mesenchymal Stem Cells
The activity of stem cell processes is regulated by internal and external signals of the cell “niche”. In general, the niche of stem cells can be represented as the microenvironment of the cells, providing a signal complex, determining the properties of the cells. At the same time, the “niche” conce...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7692241/ https://www.ncbi.nlm.nih.gov/pubmed/33143320 http://dx.doi.org/10.3390/polym12112550 |
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author | Egorikhina, Marfa N. Rubtsova, Yulia P. Charykova, Irina N. Bugrova, Marina L. Bronnikova, Irina I. Mukhina, Polina A. Sosnina, Larisa N. Aleynik, Diana Ya. |
author_facet | Egorikhina, Marfa N. Rubtsova, Yulia P. Charykova, Irina N. Bugrova, Marina L. Bronnikova, Irina I. Mukhina, Polina A. Sosnina, Larisa N. Aleynik, Diana Ya. |
author_sort | Egorikhina, Marfa N. |
collection | PubMed |
description | The activity of stem cell processes is regulated by internal and external signals of the cell “niche”. In general, the niche of stem cells can be represented as the microenvironment of the cells, providing a signal complex, determining the properties of the cells. At the same time, the “niche” concept implies feedback. Cells can modify their microenvironment, supporting homeostasis or remodeling the composition and structure of the extracellular matrix. To ensure the regenerative potential of tissue engineering products the “niche” concept should be taken into account. To investigate interactions in an experimental niche, an original hydrogel biopolymer scaffold with encapsulated mesenchymal adipose-derived stem cells (ASCs) was used in this study. The scaffold provides for cell adhesion, active cell growth, and proliferative activity. Cells cultured within a scaffold are distinguished by the presence of a developed cytoskeleton and they form a cellular network. ASCs cultured within a scaffold change their microenvironment by secreting VEGF-A and remodeling the scaffold structure. Scaffold biodegradation processes were evaluated after previous culturing of the ASCs in the scaffolds for periods of either 24 h or six days. The revealed differences confirmed that changes had occurred in the properties of scaffolds remodeled by cells during cultivation. The mechanisms of the identified changes and the possibility of considering the presented scaffold as an appropriate artificial niche for ASCs are discussed. |
format | Online Article Text |
id | pubmed-7692241 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-76922412020-11-28 Biopolymer Hydrogel Scaffold as an Artificial Cell Niche for Mesenchymal Stem Cells Egorikhina, Marfa N. Rubtsova, Yulia P. Charykova, Irina N. Bugrova, Marina L. Bronnikova, Irina I. Mukhina, Polina A. Sosnina, Larisa N. Aleynik, Diana Ya. Polymers (Basel) Article The activity of stem cell processes is regulated by internal and external signals of the cell “niche”. In general, the niche of stem cells can be represented as the microenvironment of the cells, providing a signal complex, determining the properties of the cells. At the same time, the “niche” concept implies feedback. Cells can modify their microenvironment, supporting homeostasis or remodeling the composition and structure of the extracellular matrix. To ensure the regenerative potential of tissue engineering products the “niche” concept should be taken into account. To investigate interactions in an experimental niche, an original hydrogel biopolymer scaffold with encapsulated mesenchymal adipose-derived stem cells (ASCs) was used in this study. The scaffold provides for cell adhesion, active cell growth, and proliferative activity. Cells cultured within a scaffold are distinguished by the presence of a developed cytoskeleton and they form a cellular network. ASCs cultured within a scaffold change their microenvironment by secreting VEGF-A and remodeling the scaffold structure. Scaffold biodegradation processes were evaluated after previous culturing of the ASCs in the scaffolds for periods of either 24 h or six days. The revealed differences confirmed that changes had occurred in the properties of scaffolds remodeled by cells during cultivation. The mechanisms of the identified changes and the possibility of considering the presented scaffold as an appropriate artificial niche for ASCs are discussed. MDPI 2020-10-30 /pmc/articles/PMC7692241/ /pubmed/33143320 http://dx.doi.org/10.3390/polym12112550 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Egorikhina, Marfa N. Rubtsova, Yulia P. Charykova, Irina N. Bugrova, Marina L. Bronnikova, Irina I. Mukhina, Polina A. Sosnina, Larisa N. Aleynik, Diana Ya. Biopolymer Hydrogel Scaffold as an Artificial Cell Niche for Mesenchymal Stem Cells |
title | Biopolymer Hydrogel Scaffold as an Artificial Cell Niche for Mesenchymal Stem Cells |
title_full | Biopolymer Hydrogel Scaffold as an Artificial Cell Niche for Mesenchymal Stem Cells |
title_fullStr | Biopolymer Hydrogel Scaffold as an Artificial Cell Niche for Mesenchymal Stem Cells |
title_full_unstemmed | Biopolymer Hydrogel Scaffold as an Artificial Cell Niche for Mesenchymal Stem Cells |
title_short | Biopolymer Hydrogel Scaffold as an Artificial Cell Niche for Mesenchymal Stem Cells |
title_sort | biopolymer hydrogel scaffold as an artificial cell niche for mesenchymal stem cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7692241/ https://www.ncbi.nlm.nih.gov/pubmed/33143320 http://dx.doi.org/10.3390/polym12112550 |
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