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Comprehensive Dipeptide Analysis Revealed Cancer-Specific Profile in the Liver of Patients with Hepatocellular Carcinoma and Hepatitis

As the physical properties and functionality of dipeptides differ from those of amino acids, they have attracted attention in metabolomics; however, their functions in vivo have not been clarified in detail. Hepatocellular carcinoma (HCC) is the most common type of primary liver cancer, and its majo...

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Autores principales: Ozawa, Hitoshi, Hirayama, Akiyoshi, Shoji, Futaba, Maruyama, Midori, Suzuki, Kumi, Yamanaka-Okumura, Hisami, Tatano, Hiroshi, Morine, Yuji, Soga, Tomoyoshi, Shimada, Mitsuo, Tomita, Masaru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7692321/
https://www.ncbi.nlm.nih.gov/pubmed/33139606
http://dx.doi.org/10.3390/metabo10110442
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author Ozawa, Hitoshi
Hirayama, Akiyoshi
Shoji, Futaba
Maruyama, Midori
Suzuki, Kumi
Yamanaka-Okumura, Hisami
Tatano, Hiroshi
Morine, Yuji
Soga, Tomoyoshi
Shimada, Mitsuo
Tomita, Masaru
author_facet Ozawa, Hitoshi
Hirayama, Akiyoshi
Shoji, Futaba
Maruyama, Midori
Suzuki, Kumi
Yamanaka-Okumura, Hisami
Tatano, Hiroshi
Morine, Yuji
Soga, Tomoyoshi
Shimada, Mitsuo
Tomita, Masaru
author_sort Ozawa, Hitoshi
collection PubMed
description As the physical properties and functionality of dipeptides differ from those of amino acids, they have attracted attention in metabolomics; however, their functions in vivo have not been clarified in detail. Hepatocellular carcinoma (HCC) is the most common type of primary liver cancer, and its major cause is chronic hepatitis. This study was conducted to explore tumor-specific dipeptide characteristics by performing comprehensive dipeptide analysis in the tumor and surrounding nontumor tissue of patients with HCC. Dipeptides were analyzed by liquid chromatography tandem mass spectrometry and capillary electrophoresis tandem mass spectrometry. Principal component analysis using 236 detected dipeptides showed differences in the dipeptide profiles between nontumor and tumor tissues; however, no clear difference was observed in etiological comparison. In addition, the N- and C-terminal amino acid compositions of the detected dipeptides significantly differed, suggesting the substrate specificity of enzyme proteins, such as peptidase. Furthermore, hepatitis-derived HCC may show a characteristic dipeptide profile even before tumor formation. These results provide insight into HCC pathogenesis and may help identify novel biomarkers for diagnosis.
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spelling pubmed-76923212020-11-28 Comprehensive Dipeptide Analysis Revealed Cancer-Specific Profile in the Liver of Patients with Hepatocellular Carcinoma and Hepatitis Ozawa, Hitoshi Hirayama, Akiyoshi Shoji, Futaba Maruyama, Midori Suzuki, Kumi Yamanaka-Okumura, Hisami Tatano, Hiroshi Morine, Yuji Soga, Tomoyoshi Shimada, Mitsuo Tomita, Masaru Metabolites Article As the physical properties and functionality of dipeptides differ from those of amino acids, they have attracted attention in metabolomics; however, their functions in vivo have not been clarified in detail. Hepatocellular carcinoma (HCC) is the most common type of primary liver cancer, and its major cause is chronic hepatitis. This study was conducted to explore tumor-specific dipeptide characteristics by performing comprehensive dipeptide analysis in the tumor and surrounding nontumor tissue of patients with HCC. Dipeptides were analyzed by liquid chromatography tandem mass spectrometry and capillary electrophoresis tandem mass spectrometry. Principal component analysis using 236 detected dipeptides showed differences in the dipeptide profiles between nontumor and tumor tissues; however, no clear difference was observed in etiological comparison. In addition, the N- and C-terminal amino acid compositions of the detected dipeptides significantly differed, suggesting the substrate specificity of enzyme proteins, such as peptidase. Furthermore, hepatitis-derived HCC may show a characteristic dipeptide profile even before tumor formation. These results provide insight into HCC pathogenesis and may help identify novel biomarkers for diagnosis. MDPI 2020-11-01 /pmc/articles/PMC7692321/ /pubmed/33139606 http://dx.doi.org/10.3390/metabo10110442 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ozawa, Hitoshi
Hirayama, Akiyoshi
Shoji, Futaba
Maruyama, Midori
Suzuki, Kumi
Yamanaka-Okumura, Hisami
Tatano, Hiroshi
Morine, Yuji
Soga, Tomoyoshi
Shimada, Mitsuo
Tomita, Masaru
Comprehensive Dipeptide Analysis Revealed Cancer-Specific Profile in the Liver of Patients with Hepatocellular Carcinoma and Hepatitis
title Comprehensive Dipeptide Analysis Revealed Cancer-Specific Profile in the Liver of Patients with Hepatocellular Carcinoma and Hepatitis
title_full Comprehensive Dipeptide Analysis Revealed Cancer-Specific Profile in the Liver of Patients with Hepatocellular Carcinoma and Hepatitis
title_fullStr Comprehensive Dipeptide Analysis Revealed Cancer-Specific Profile in the Liver of Patients with Hepatocellular Carcinoma and Hepatitis
title_full_unstemmed Comprehensive Dipeptide Analysis Revealed Cancer-Specific Profile in the Liver of Patients with Hepatocellular Carcinoma and Hepatitis
title_short Comprehensive Dipeptide Analysis Revealed Cancer-Specific Profile in the Liver of Patients with Hepatocellular Carcinoma and Hepatitis
title_sort comprehensive dipeptide analysis revealed cancer-specific profile in the liver of patients with hepatocellular carcinoma and hepatitis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7692321/
https://www.ncbi.nlm.nih.gov/pubmed/33139606
http://dx.doi.org/10.3390/metabo10110442
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