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Nitrate-Induced CLE Peptide Systemically Inhibits Nodulation in Medicago truncatula
Legume plants form nitrogen-fixing nodules in symbiosis with soil bacteria rhizobia. The number of symbiotic nodules is controlled at the whole-plant level with autoregulation of nodulation (AON), which includes a shoot-acting CLV1-like receptor kinase and mobile CLE (CLAVATA3/ENDOSPERM SURROUNDING...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7692534/ https://www.ncbi.nlm.nih.gov/pubmed/33126720 http://dx.doi.org/10.3390/plants9111456 |
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author | Lebedeva, Maria Azarakhsh, Mahboobeh Yashenkova, Yaroslavna Lutova, Lyudmila |
author_facet | Lebedeva, Maria Azarakhsh, Mahboobeh Yashenkova, Yaroslavna Lutova, Lyudmila |
author_sort | Lebedeva, Maria |
collection | PubMed |
description | Legume plants form nitrogen-fixing nodules in symbiosis with soil bacteria rhizobia. The number of symbiotic nodules is controlled at the whole-plant level with autoregulation of nodulation (AON), which includes a shoot-acting CLV1-like receptor kinase and mobile CLE (CLAVATA3/ENDOSPERM SURROUNDING REGION-related) peptides that are produced in the root in response to rhizobia inoculation. In addition to rhizobia-induced CLE peptides, nitrate-induced CLE genes have been identified in Lotus japonicus and Glycine max, which inhibited nodulation when overexpressed. However, nitrate-induced CLE genes that systemically suppress nodulation in AON-dependent manner have not been identified in Medicago truncatula. Here, we found that MtCLE35 expression is activated by both rhizobia inoculation and nitrate treatment in M. truncatula, similarly to L. japonicus CLE genes. Moreover, we found that MtCLE35 systemically suppresses nodulation in AON-dependent manner, suggesting that MtCLE35 may mediate nitrate-induced inhibition of nodulation in M. truncatula. |
format | Online Article Text |
id | pubmed-7692534 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-76925342020-11-28 Nitrate-Induced CLE Peptide Systemically Inhibits Nodulation in Medicago truncatula Lebedeva, Maria Azarakhsh, Mahboobeh Yashenkova, Yaroslavna Lutova, Lyudmila Plants (Basel) Article Legume plants form nitrogen-fixing nodules in symbiosis with soil bacteria rhizobia. The number of symbiotic nodules is controlled at the whole-plant level with autoregulation of nodulation (AON), which includes a shoot-acting CLV1-like receptor kinase and mobile CLE (CLAVATA3/ENDOSPERM SURROUNDING REGION-related) peptides that are produced in the root in response to rhizobia inoculation. In addition to rhizobia-induced CLE peptides, nitrate-induced CLE genes have been identified in Lotus japonicus and Glycine max, which inhibited nodulation when overexpressed. However, nitrate-induced CLE genes that systemically suppress nodulation in AON-dependent manner have not been identified in Medicago truncatula. Here, we found that MtCLE35 expression is activated by both rhizobia inoculation and nitrate treatment in M. truncatula, similarly to L. japonicus CLE genes. Moreover, we found that MtCLE35 systemically suppresses nodulation in AON-dependent manner, suggesting that MtCLE35 may mediate nitrate-induced inhibition of nodulation in M. truncatula. MDPI 2020-10-28 /pmc/articles/PMC7692534/ /pubmed/33126720 http://dx.doi.org/10.3390/plants9111456 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Lebedeva, Maria Azarakhsh, Mahboobeh Yashenkova, Yaroslavna Lutova, Lyudmila Nitrate-Induced CLE Peptide Systemically Inhibits Nodulation in Medicago truncatula |
title | Nitrate-Induced CLE Peptide Systemically Inhibits Nodulation in Medicago truncatula |
title_full | Nitrate-Induced CLE Peptide Systemically Inhibits Nodulation in Medicago truncatula |
title_fullStr | Nitrate-Induced CLE Peptide Systemically Inhibits Nodulation in Medicago truncatula |
title_full_unstemmed | Nitrate-Induced CLE Peptide Systemically Inhibits Nodulation in Medicago truncatula |
title_short | Nitrate-Induced CLE Peptide Systemically Inhibits Nodulation in Medicago truncatula |
title_sort | nitrate-induced cle peptide systemically inhibits nodulation in medicago truncatula |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7692534/ https://www.ncbi.nlm.nih.gov/pubmed/33126720 http://dx.doi.org/10.3390/plants9111456 |
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