Cargando…

Expression of Genes Encoding Manganese Peroxidase and Laccase of Ganoderma boninense in Response to Nitrogen Sources, Hydrogen Peroxide and Phytohormones

Ganoderma produces lignolytic enzymes that can degrade the lignin component of plant cell walls, causing basal stem rot to oil palms. Nitrogen sources may affect plant tolerance to root pathogens while hydrogen peroxide (H(2)O(2)), salicylic acid (SA) and jasmonic acid (JA) play important roles in p...

Descripción completa

Detalles Bibliográficos
Autores principales: Ho, Pei-Yin, Namasivayam, Parameswari, Sundram, Shamala, Ho, Chai-Ling
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7692562/
https://www.ncbi.nlm.nih.gov/pubmed/33114747
http://dx.doi.org/10.3390/genes11111263
_version_ 1783614540995362816
author Ho, Pei-Yin
Namasivayam, Parameswari
Sundram, Shamala
Ho, Chai-Ling
author_facet Ho, Pei-Yin
Namasivayam, Parameswari
Sundram, Shamala
Ho, Chai-Ling
author_sort Ho, Pei-Yin
collection PubMed
description Ganoderma produces lignolytic enzymes that can degrade the lignin component of plant cell walls, causing basal stem rot to oil palms. Nitrogen sources may affect plant tolerance to root pathogens while hydrogen peroxide (H(2)O(2)), salicylic acid (SA) and jasmonic acid (JA) play important roles in plant defense against pathogens. In this study, we examined the expression of genes encoding manganese peroxidase (MnP) and laccase (Lac) in Ganoderma boninense treated with different nitrogen sources (ammonium nitrate, ammonium sulphate, sodium nitrate and potassium nitrate), JA, SA and H(2)O(2). Transcripts encoding MnP and Lac were cloned from G. boninense. Of the three GbMnP genes, GbMnP_U6011 was up-regulated by all nitrogen sources examined and H(2)O(2) but was down-regulated by JA. The expression of GbMnP_U87 was only up-regulated by JA while GbMnP_35959 was up-regulated by ammonium nitrate but suppressed by sodium nitrate and down-regulated by H(2)O(2). Among the three GbLac genes examined, GbLac_U90667 was up-regulated by ammonium nitrate, JA, SA and H(2)O(2); GbLac_U36023 was up-regulated by JA and H(2)O(2) while GbLac_U30636 was up-regulated by SA but suppressed by ammonium sulphate, sodium nitrate, JA and H(2)O(2). Differential expression of these genes may be required by their different functional roles in G. boninense.
format Online
Article
Text
id pubmed-7692562
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-76925622020-11-28 Expression of Genes Encoding Manganese Peroxidase and Laccase of Ganoderma boninense in Response to Nitrogen Sources, Hydrogen Peroxide and Phytohormones Ho, Pei-Yin Namasivayam, Parameswari Sundram, Shamala Ho, Chai-Ling Genes (Basel) Article Ganoderma produces lignolytic enzymes that can degrade the lignin component of plant cell walls, causing basal stem rot to oil palms. Nitrogen sources may affect plant tolerance to root pathogens while hydrogen peroxide (H(2)O(2)), salicylic acid (SA) and jasmonic acid (JA) play important roles in plant defense against pathogens. In this study, we examined the expression of genes encoding manganese peroxidase (MnP) and laccase (Lac) in Ganoderma boninense treated with different nitrogen sources (ammonium nitrate, ammonium sulphate, sodium nitrate and potassium nitrate), JA, SA and H(2)O(2). Transcripts encoding MnP and Lac were cloned from G. boninense. Of the three GbMnP genes, GbMnP_U6011 was up-regulated by all nitrogen sources examined and H(2)O(2) but was down-regulated by JA. The expression of GbMnP_U87 was only up-regulated by JA while GbMnP_35959 was up-regulated by ammonium nitrate but suppressed by sodium nitrate and down-regulated by H(2)O(2). Among the three GbLac genes examined, GbLac_U90667 was up-regulated by ammonium nitrate, JA, SA and H(2)O(2); GbLac_U36023 was up-regulated by JA and H(2)O(2) while GbLac_U30636 was up-regulated by SA but suppressed by ammonium sulphate, sodium nitrate, JA and H(2)O(2). Differential expression of these genes may be required by their different functional roles in G. boninense. MDPI 2020-10-26 /pmc/articles/PMC7692562/ /pubmed/33114747 http://dx.doi.org/10.3390/genes11111263 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ho, Pei-Yin
Namasivayam, Parameswari
Sundram, Shamala
Ho, Chai-Ling
Expression of Genes Encoding Manganese Peroxidase and Laccase of Ganoderma boninense in Response to Nitrogen Sources, Hydrogen Peroxide and Phytohormones
title Expression of Genes Encoding Manganese Peroxidase and Laccase of Ganoderma boninense in Response to Nitrogen Sources, Hydrogen Peroxide and Phytohormones
title_full Expression of Genes Encoding Manganese Peroxidase and Laccase of Ganoderma boninense in Response to Nitrogen Sources, Hydrogen Peroxide and Phytohormones
title_fullStr Expression of Genes Encoding Manganese Peroxidase and Laccase of Ganoderma boninense in Response to Nitrogen Sources, Hydrogen Peroxide and Phytohormones
title_full_unstemmed Expression of Genes Encoding Manganese Peroxidase and Laccase of Ganoderma boninense in Response to Nitrogen Sources, Hydrogen Peroxide and Phytohormones
title_short Expression of Genes Encoding Manganese Peroxidase and Laccase of Ganoderma boninense in Response to Nitrogen Sources, Hydrogen Peroxide and Phytohormones
title_sort expression of genes encoding manganese peroxidase and laccase of ganoderma boninense in response to nitrogen sources, hydrogen peroxide and phytohormones
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7692562/
https://www.ncbi.nlm.nih.gov/pubmed/33114747
http://dx.doi.org/10.3390/genes11111263
work_keys_str_mv AT hopeiyin expressionofgenesencodingmanganeseperoxidaseandlaccaseofganodermaboninenseinresponsetonitrogensourceshydrogenperoxideandphytohormones
AT namasivayamparameswari expressionofgenesencodingmanganeseperoxidaseandlaccaseofganodermaboninenseinresponsetonitrogensourceshydrogenperoxideandphytohormones
AT sundramshamala expressionofgenesencodingmanganeseperoxidaseandlaccaseofganodermaboninenseinresponsetonitrogensourceshydrogenperoxideandphytohormones
AT hochailing expressionofgenesencodingmanganeseperoxidaseandlaccaseofganodermaboninenseinresponsetonitrogensourceshydrogenperoxideandphytohormones