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Antibody Binding to SARS-CoV-2 S Glycoprotein Correlates with but Does Not Predict Neutralization
Convalescent plasma from SARS-CoV-2 infected individuals and monoclonal antibodies were shown to potently neutralize viral and pseudoviral particles carrying the S glycoprotein. However, a non-negligent proportion of plasma samples from infected individuals, as well as S-specific monoclonal antibodi...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7692607/ https://www.ncbi.nlm.nih.gov/pubmed/33114742 http://dx.doi.org/10.3390/v12111214 |
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author | Ding, Shilei Laumaea, Annemarie Benlarbi, Mehdi Beaudoin-Bussières, Guillaume Gasser, Romain Medjahed, Halima Pancera, Marie Stamatatos, Leonidas McGuire, Andrew T. Bazin, Renée Finzi, Andrés |
author_facet | Ding, Shilei Laumaea, Annemarie Benlarbi, Mehdi Beaudoin-Bussières, Guillaume Gasser, Romain Medjahed, Halima Pancera, Marie Stamatatos, Leonidas McGuire, Andrew T. Bazin, Renée Finzi, Andrés |
author_sort | Ding, Shilei |
collection | PubMed |
description | Convalescent plasma from SARS-CoV-2 infected individuals and monoclonal antibodies were shown to potently neutralize viral and pseudoviral particles carrying the S glycoprotein. However, a non-negligent proportion of plasma samples from infected individuals, as well as S-specific monoclonal antibodies, were reported to be non-neutralizing despite efficient interaction with the S glycoprotein in different biochemical assays using soluble recombinant forms of S or when expressed at the cell surface. How neutralization relates to the binding of S glycoprotein in the context of viral particles remains to be established. Here, we developed a pseudovirus capture assay (VCA) to measure the capacity of plasma samples or antibodies immobilized on ELISA plates to bind to membrane-bound S glycoproteins from SARS-CoV-2 expressed at the surface of lentiviral particles. By performing VCA, ELISA, and neutralization assays, we observed a strong correlation between these parameters. However, while we found that plasma samples unable to capture viral particles did not neutralize, capture did not guarantee neutralization, indicating that the capacity of antibodies to bind to the S glycoprotein at the surface of pseudoviral particles is required but not sufficient to mediate neutralization. Altogether, our results highlight the importance of better understanding the inactivation of S by plasma and neutralizing antibodies. |
format | Online Article Text |
id | pubmed-7692607 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-76926072020-11-28 Antibody Binding to SARS-CoV-2 S Glycoprotein Correlates with but Does Not Predict Neutralization Ding, Shilei Laumaea, Annemarie Benlarbi, Mehdi Beaudoin-Bussières, Guillaume Gasser, Romain Medjahed, Halima Pancera, Marie Stamatatos, Leonidas McGuire, Andrew T. Bazin, Renée Finzi, Andrés Viruses Communication Convalescent plasma from SARS-CoV-2 infected individuals and monoclonal antibodies were shown to potently neutralize viral and pseudoviral particles carrying the S glycoprotein. However, a non-negligent proportion of plasma samples from infected individuals, as well as S-specific monoclonal antibodies, were reported to be non-neutralizing despite efficient interaction with the S glycoprotein in different biochemical assays using soluble recombinant forms of S or when expressed at the cell surface. How neutralization relates to the binding of S glycoprotein in the context of viral particles remains to be established. Here, we developed a pseudovirus capture assay (VCA) to measure the capacity of plasma samples or antibodies immobilized on ELISA plates to bind to membrane-bound S glycoproteins from SARS-CoV-2 expressed at the surface of lentiviral particles. By performing VCA, ELISA, and neutralization assays, we observed a strong correlation between these parameters. However, while we found that plasma samples unable to capture viral particles did not neutralize, capture did not guarantee neutralization, indicating that the capacity of antibodies to bind to the S glycoprotein at the surface of pseudoviral particles is required but not sufficient to mediate neutralization. Altogether, our results highlight the importance of better understanding the inactivation of S by plasma and neutralizing antibodies. MDPI 2020-10-26 /pmc/articles/PMC7692607/ /pubmed/33114742 http://dx.doi.org/10.3390/v12111214 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Ding, Shilei Laumaea, Annemarie Benlarbi, Mehdi Beaudoin-Bussières, Guillaume Gasser, Romain Medjahed, Halima Pancera, Marie Stamatatos, Leonidas McGuire, Andrew T. Bazin, Renée Finzi, Andrés Antibody Binding to SARS-CoV-2 S Glycoprotein Correlates with but Does Not Predict Neutralization |
title | Antibody Binding to SARS-CoV-2 S Glycoprotein Correlates with but Does Not Predict Neutralization |
title_full | Antibody Binding to SARS-CoV-2 S Glycoprotein Correlates with but Does Not Predict Neutralization |
title_fullStr | Antibody Binding to SARS-CoV-2 S Glycoprotein Correlates with but Does Not Predict Neutralization |
title_full_unstemmed | Antibody Binding to SARS-CoV-2 S Glycoprotein Correlates with but Does Not Predict Neutralization |
title_short | Antibody Binding to SARS-CoV-2 S Glycoprotein Correlates with but Does Not Predict Neutralization |
title_sort | antibody binding to sars-cov-2 s glycoprotein correlates with but does not predict neutralization |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7692607/ https://www.ncbi.nlm.nih.gov/pubmed/33114742 http://dx.doi.org/10.3390/v12111214 |
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