Cargando…

Transcriptomic and Proteomic Analysis Reveals Mechanisms of Patulin-Induced Cell Toxicity in Human Embryonic Kidney Cells

Patulin (PAT) is a natural mycotoxin that commonly contaminates fruits and fruit-based products. Previous work indicated that PAT-induced apoptosis in which reactive oxygen species (ROS) are involved in human embryonic kidney (HEK293) cells. To uncover novel aspects of the possible mechanism of PAT...

Descripción completa

Detalles Bibliográficos
Autores principales: Han, Nianfa, Luo, Ruilin, Liu, Jiayu, Guo, Tianmin, Feng, Jiayu, Peng, Xiaoli
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7692636/
https://www.ncbi.nlm.nih.gov/pubmed/33138038
http://dx.doi.org/10.3390/toxins12110681
_version_ 1783614558196203520
author Han, Nianfa
Luo, Ruilin
Liu, Jiayu
Guo, Tianmin
Feng, Jiayu
Peng, Xiaoli
author_facet Han, Nianfa
Luo, Ruilin
Liu, Jiayu
Guo, Tianmin
Feng, Jiayu
Peng, Xiaoli
author_sort Han, Nianfa
collection PubMed
description Patulin (PAT) is a natural mycotoxin that commonly contaminates fruits and fruit-based products. Previous work indicated that PAT-induced apoptosis in which reactive oxygen species (ROS) are involved in human embryonic kidney (HEK293) cells. To uncover novel aspects of the possible mechanism of PAT nephrotoxicity, the transcriptome and proteome profiles were investigated using the digital gene expression (DGE) and isobaric tags for relative and absolute quantitation (iTRAQ) proteomic approaches. A total of 127 genes and 85 proteins were found to express differentially in response to 5 μM PAT for 10 h in HEK293 cells. The most dramatic changes of expression were noticed with genes or proteins related to apoptosis, oxidative phosphorylation ribosome and cell cycle. Especially, the activation of caspase 3, UQCR11, active transport form and endocytosis appeared to be crucial in PAT kidney cytotoxicity. PAT also seemed to be associated with cancer and neuropathic disease as pathways associated with carcinogenesis, Alzheimer’s disease and Parkinson’s disease were induced. Overall, this study served to uncover overall insights associated with signaling pathway that modulated the PAT toxicity mechanism.
format Online
Article
Text
id pubmed-7692636
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-76926362020-11-28 Transcriptomic and Proteomic Analysis Reveals Mechanisms of Patulin-Induced Cell Toxicity in Human Embryonic Kidney Cells Han, Nianfa Luo, Ruilin Liu, Jiayu Guo, Tianmin Feng, Jiayu Peng, Xiaoli Toxins (Basel) Article Patulin (PAT) is a natural mycotoxin that commonly contaminates fruits and fruit-based products. Previous work indicated that PAT-induced apoptosis in which reactive oxygen species (ROS) are involved in human embryonic kidney (HEK293) cells. To uncover novel aspects of the possible mechanism of PAT nephrotoxicity, the transcriptome and proteome profiles were investigated using the digital gene expression (DGE) and isobaric tags for relative and absolute quantitation (iTRAQ) proteomic approaches. A total of 127 genes and 85 proteins were found to express differentially in response to 5 μM PAT for 10 h in HEK293 cells. The most dramatic changes of expression were noticed with genes or proteins related to apoptosis, oxidative phosphorylation ribosome and cell cycle. Especially, the activation of caspase 3, UQCR11, active transport form and endocytosis appeared to be crucial in PAT kidney cytotoxicity. PAT also seemed to be associated with cancer and neuropathic disease as pathways associated with carcinogenesis, Alzheimer’s disease and Parkinson’s disease were induced. Overall, this study served to uncover overall insights associated with signaling pathway that modulated the PAT toxicity mechanism. MDPI 2020-10-29 /pmc/articles/PMC7692636/ /pubmed/33138038 http://dx.doi.org/10.3390/toxins12110681 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Han, Nianfa
Luo, Ruilin
Liu, Jiayu
Guo, Tianmin
Feng, Jiayu
Peng, Xiaoli
Transcriptomic and Proteomic Analysis Reveals Mechanisms of Patulin-Induced Cell Toxicity in Human Embryonic Kidney Cells
title Transcriptomic and Proteomic Analysis Reveals Mechanisms of Patulin-Induced Cell Toxicity in Human Embryonic Kidney Cells
title_full Transcriptomic and Proteomic Analysis Reveals Mechanisms of Patulin-Induced Cell Toxicity in Human Embryonic Kidney Cells
title_fullStr Transcriptomic and Proteomic Analysis Reveals Mechanisms of Patulin-Induced Cell Toxicity in Human Embryonic Kidney Cells
title_full_unstemmed Transcriptomic and Proteomic Analysis Reveals Mechanisms of Patulin-Induced Cell Toxicity in Human Embryonic Kidney Cells
title_short Transcriptomic and Proteomic Analysis Reveals Mechanisms of Patulin-Induced Cell Toxicity in Human Embryonic Kidney Cells
title_sort transcriptomic and proteomic analysis reveals mechanisms of patulin-induced cell toxicity in human embryonic kidney cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7692636/
https://www.ncbi.nlm.nih.gov/pubmed/33138038
http://dx.doi.org/10.3390/toxins12110681
work_keys_str_mv AT hannianfa transcriptomicandproteomicanalysisrevealsmechanismsofpatulininducedcelltoxicityinhumanembryonickidneycells
AT luoruilin transcriptomicandproteomicanalysisrevealsmechanismsofpatulininducedcelltoxicityinhumanembryonickidneycells
AT liujiayu transcriptomicandproteomicanalysisrevealsmechanismsofpatulininducedcelltoxicityinhumanembryonickidneycells
AT guotianmin transcriptomicandproteomicanalysisrevealsmechanismsofpatulininducedcelltoxicityinhumanembryonickidneycells
AT fengjiayu transcriptomicandproteomicanalysisrevealsmechanismsofpatulininducedcelltoxicityinhumanembryonickidneycells
AT pengxiaoli transcriptomicandproteomicanalysisrevealsmechanismsofpatulininducedcelltoxicityinhumanembryonickidneycells