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First Molecular Detection of Babesia ovis, Theileria spp., Anaplasma spp., and Ehrlichia ruminantium in Goats from Western Uganda
Ticks and tick-borne diseases are major impediments to livestock production. To date, there have been several studies on the prevalence of tick-borne pathogens (TBPs) in cattle, but very few studies have documented TBPs in goats in Uganda. In this study, polymerase chain reaction assays and sequence...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7692732/ https://www.ncbi.nlm.nih.gov/pubmed/33121172 http://dx.doi.org/10.3390/pathogens9110895 |
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author | Tumwebaze, Maria Agnes Byamukama, Benedicto Tayebwa, Dickson Stuart Byaruhanga, Joseph Angwe, Martin Kamilo Galon, Eloiza May Liu, Mingming Lee, Seung-Hun Ringo, Aaron Edmond Adjou Moumouni, Paul Franck Li, Jixu Li, Yongchang Ji, Shengwei Vudriko, Patrick Xuan, Xuenan |
author_facet | Tumwebaze, Maria Agnes Byamukama, Benedicto Tayebwa, Dickson Stuart Byaruhanga, Joseph Angwe, Martin Kamilo Galon, Eloiza May Liu, Mingming Lee, Seung-Hun Ringo, Aaron Edmond Adjou Moumouni, Paul Franck Li, Jixu Li, Yongchang Ji, Shengwei Vudriko, Patrick Xuan, Xuenan |
author_sort | Tumwebaze, Maria Agnes |
collection | PubMed |
description | Ticks and tick-borne diseases are major impediments to livestock production. To date, there have been several studies on the prevalence of tick-borne pathogens (TBPs) in cattle, but very few studies have documented TBPs in goats in Uganda. In this study, polymerase chain reaction assays and sequence analysis of different molecular markers were used to assess the presence and genetic characteristics of TBPs in 201 goats from Kasese district in western Uganda. The risk factors associated with TBP infections were also analyzed. We detected Theileria spp. (13.4%), Anaplasma phagocytophilum (10.9%), Anaplasma ovis (5.5%), Babesia ovis (5.5%), and Ehrlichia ruminantium (0.5%). The sequences of B. ovis ssu rRNA and A. ovis msp4 genes showed some degree of diversity among the parasite isolates in this study. The E. ruminantium pCS20 sequence formed a well-supported clade with isolates from Amblyomma variegatum ticks from Uganda. Wildlife interaction, sampling location, low body condition score, tick infestation, and herd size were significantly associated with TBP infections in the goats. The findings in this study provide important information on the epidemiology of tick-borne pathogens in Uganda, and show that goats could be potential reservoirs for tick-borne pathogens. |
format | Online Article Text |
id | pubmed-7692732 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-76927322020-11-28 First Molecular Detection of Babesia ovis, Theileria spp., Anaplasma spp., and Ehrlichia ruminantium in Goats from Western Uganda Tumwebaze, Maria Agnes Byamukama, Benedicto Tayebwa, Dickson Stuart Byaruhanga, Joseph Angwe, Martin Kamilo Galon, Eloiza May Liu, Mingming Lee, Seung-Hun Ringo, Aaron Edmond Adjou Moumouni, Paul Franck Li, Jixu Li, Yongchang Ji, Shengwei Vudriko, Patrick Xuan, Xuenan Pathogens Article Ticks and tick-borne diseases are major impediments to livestock production. To date, there have been several studies on the prevalence of tick-borne pathogens (TBPs) in cattle, but very few studies have documented TBPs in goats in Uganda. In this study, polymerase chain reaction assays and sequence analysis of different molecular markers were used to assess the presence and genetic characteristics of TBPs in 201 goats from Kasese district in western Uganda. The risk factors associated with TBP infections were also analyzed. We detected Theileria spp. (13.4%), Anaplasma phagocytophilum (10.9%), Anaplasma ovis (5.5%), Babesia ovis (5.5%), and Ehrlichia ruminantium (0.5%). The sequences of B. ovis ssu rRNA and A. ovis msp4 genes showed some degree of diversity among the parasite isolates in this study. The E. ruminantium pCS20 sequence formed a well-supported clade with isolates from Amblyomma variegatum ticks from Uganda. Wildlife interaction, sampling location, low body condition score, tick infestation, and herd size were significantly associated with TBP infections in the goats. The findings in this study provide important information on the epidemiology of tick-borne pathogens in Uganda, and show that goats could be potential reservoirs for tick-borne pathogens. MDPI 2020-10-27 /pmc/articles/PMC7692732/ /pubmed/33121172 http://dx.doi.org/10.3390/pathogens9110895 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Tumwebaze, Maria Agnes Byamukama, Benedicto Tayebwa, Dickson Stuart Byaruhanga, Joseph Angwe, Martin Kamilo Galon, Eloiza May Liu, Mingming Lee, Seung-Hun Ringo, Aaron Edmond Adjou Moumouni, Paul Franck Li, Jixu Li, Yongchang Ji, Shengwei Vudriko, Patrick Xuan, Xuenan First Molecular Detection of Babesia ovis, Theileria spp., Anaplasma spp., and Ehrlichia ruminantium in Goats from Western Uganda |
title | First Molecular Detection of Babesia ovis, Theileria spp., Anaplasma spp., and Ehrlichia ruminantium in Goats from Western Uganda |
title_full | First Molecular Detection of Babesia ovis, Theileria spp., Anaplasma spp., and Ehrlichia ruminantium in Goats from Western Uganda |
title_fullStr | First Molecular Detection of Babesia ovis, Theileria spp., Anaplasma spp., and Ehrlichia ruminantium in Goats from Western Uganda |
title_full_unstemmed | First Molecular Detection of Babesia ovis, Theileria spp., Anaplasma spp., and Ehrlichia ruminantium in Goats from Western Uganda |
title_short | First Molecular Detection of Babesia ovis, Theileria spp., Anaplasma spp., and Ehrlichia ruminantium in Goats from Western Uganda |
title_sort | first molecular detection of babesia ovis, theileria spp., anaplasma spp., and ehrlichia ruminantium in goats from western uganda |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7692732/ https://www.ncbi.nlm.nih.gov/pubmed/33121172 http://dx.doi.org/10.3390/pathogens9110895 |
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