Cargando…

Salivary proteotypes of gingivitis tolerance and resilience

AIM: This study aimed to characterize the salivary proteome during the induction and resolution of gingival inflammation in the course of human experimental gingivitis (EG), and to cluster the proteomic profiles based on the clinically defined “slow” and “fast” response patterns. MATERIALS AND METHO...

Descripción completa

Detalles Bibliográficos
Autores principales: Bostanci, Nagihan, Silbereisen, Angelika, Bao, Kai, Grossmann, Jonas, Nanni, Paolo, Fernandez, Claudia, Nascimento, Gustavo G., Belibasakis, Georgios N., Lopez, Rodrigo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7692908/
https://www.ncbi.nlm.nih.gov/pubmed/32777086
http://dx.doi.org/10.1111/jcpe.13358
_version_ 1783614621902438400
author Bostanci, Nagihan
Silbereisen, Angelika
Bao, Kai
Grossmann, Jonas
Nanni, Paolo
Fernandez, Claudia
Nascimento, Gustavo G.
Belibasakis, Georgios N.
Lopez, Rodrigo
author_facet Bostanci, Nagihan
Silbereisen, Angelika
Bao, Kai
Grossmann, Jonas
Nanni, Paolo
Fernandez, Claudia
Nascimento, Gustavo G.
Belibasakis, Georgios N.
Lopez, Rodrigo
author_sort Bostanci, Nagihan
collection PubMed
description AIM: This study aimed to characterize the salivary proteome during the induction and resolution of gingival inflammation in the course of human experimental gingivitis (EG), and to cluster the proteomic profiles based on the clinically defined “slow” and “fast” response patterns. MATERIALS AND METHODS: A total of 50 unstimulated whole saliva were obtained from the EG model which was induced over 21 days (days 0, 7, 14 and 21), followed by a two‐week resolution phase (day 35). Label‐free quantitative proteomics using liquid chromatography–tandem mass spectrometry was applied. Regulated proteins were subject to Gene Ontology enrichment analysis. RESULTS: A total of 804 human proteins were quantified by ≥ 2 peptides. Principal component analysis depicted significant differences between “fast” and “slow” responders. Despite gingival and plaque scores being similar at baseline among the two groups, “fast” responders presented with 48 proteins that were at > 4‐fold higher levels than “slow” responders. These up‐regulated proteins showed enrichment in “antigen presentation” and “proteolysis.” CONCLUSIONS: Together, these findings highlight the utility of integrative systems‐level quantitative proteomic approaches to unravel the molecular basis of “salivary proteotypes” associated with gingivitis dubbed as “fast” and “slow” responders. Hence, these differential responses may help prognosticate individual susceptibility to gingival inflammation.
format Online
Article
Text
id pubmed-7692908
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher John Wiley and Sons Inc.
record_format MEDLINE/PubMed
spelling pubmed-76929082020-12-08 Salivary proteotypes of gingivitis tolerance and resilience Bostanci, Nagihan Silbereisen, Angelika Bao, Kai Grossmann, Jonas Nanni, Paolo Fernandez, Claudia Nascimento, Gustavo G. Belibasakis, Georgios N. Lopez, Rodrigo J Clin Periodontol Periodontal Health & Diseases AIM: This study aimed to characterize the salivary proteome during the induction and resolution of gingival inflammation in the course of human experimental gingivitis (EG), and to cluster the proteomic profiles based on the clinically defined “slow” and “fast” response patterns. MATERIALS AND METHODS: A total of 50 unstimulated whole saliva were obtained from the EG model which was induced over 21 days (days 0, 7, 14 and 21), followed by a two‐week resolution phase (day 35). Label‐free quantitative proteomics using liquid chromatography–tandem mass spectrometry was applied. Regulated proteins were subject to Gene Ontology enrichment analysis. RESULTS: A total of 804 human proteins were quantified by ≥ 2 peptides. Principal component analysis depicted significant differences between “fast” and “slow” responders. Despite gingival and plaque scores being similar at baseline among the two groups, “fast” responders presented with 48 proteins that were at > 4‐fold higher levels than “slow” responders. These up‐regulated proteins showed enrichment in “antigen presentation” and “proteolysis.” CONCLUSIONS: Together, these findings highlight the utility of integrative systems‐level quantitative proteomic approaches to unravel the molecular basis of “salivary proteotypes” associated with gingivitis dubbed as “fast” and “slow” responders. Hence, these differential responses may help prognosticate individual susceptibility to gingival inflammation. John Wiley and Sons Inc. 2020-09-16 2020-11 /pmc/articles/PMC7692908/ /pubmed/32777086 http://dx.doi.org/10.1111/jcpe.13358 Text en © 2020 The Authors. Journal of Clinical Periodontology published by John Wiley & Sons Ltd This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Periodontal Health & Diseases
Bostanci, Nagihan
Silbereisen, Angelika
Bao, Kai
Grossmann, Jonas
Nanni, Paolo
Fernandez, Claudia
Nascimento, Gustavo G.
Belibasakis, Georgios N.
Lopez, Rodrigo
Salivary proteotypes of gingivitis tolerance and resilience
title Salivary proteotypes of gingivitis tolerance and resilience
title_full Salivary proteotypes of gingivitis tolerance and resilience
title_fullStr Salivary proteotypes of gingivitis tolerance and resilience
title_full_unstemmed Salivary proteotypes of gingivitis tolerance and resilience
title_short Salivary proteotypes of gingivitis tolerance and resilience
title_sort salivary proteotypes of gingivitis tolerance and resilience
topic Periodontal Health & Diseases
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7692908/
https://www.ncbi.nlm.nih.gov/pubmed/32777086
http://dx.doi.org/10.1111/jcpe.13358
work_keys_str_mv AT bostancinagihan salivaryproteotypesofgingivitistoleranceandresilience
AT silbereisenangelika salivaryproteotypesofgingivitistoleranceandresilience
AT baokai salivaryproteotypesofgingivitistoleranceandresilience
AT grossmannjonas salivaryproteotypesofgingivitistoleranceandresilience
AT nannipaolo salivaryproteotypesofgingivitistoleranceandresilience
AT fernandezclaudia salivaryproteotypesofgingivitistoleranceandresilience
AT nascimentogustavog salivaryproteotypesofgingivitistoleranceandresilience
AT belibasakisgeorgiosn salivaryproteotypesofgingivitistoleranceandresilience
AT lopezrodrigo salivaryproteotypesofgingivitistoleranceandresilience