Cargando…

Sex hormone‐binding globulin regulates glucose metabolism in human placental trophoblasts via cAMP/PKA/CREB1

AIM: This study was conducted to investigate the effects of sex hormone‐binding globulin (SHBG) on glucose metabolism and insulin resistance in human placental trophoblasts and involvement of the cAMP/PKA/CREB1 signaling pathway in these effects. METHODS: An insulin resistance cell model of human tr...

Descripción completa

Detalles Bibliográficos
Autores principales: Chi, Xinshu, Feng, Chong, Wang, Xiaoyan, Jin, Zhen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons Australia, Ltd 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7692910/
https://www.ncbi.nlm.nih.gov/pubmed/32830408
http://dx.doi.org/10.1111/jog.14429
_version_ 1783614622363811840
author Chi, Xinshu
Feng, Chong
Wang, Xiaoyan
Jin, Zhen
author_facet Chi, Xinshu
Feng, Chong
Wang, Xiaoyan
Jin, Zhen
author_sort Chi, Xinshu
collection PubMed
description AIM: This study was conducted to investigate the effects of sex hormone‐binding globulin (SHBG) on glucose metabolism and insulin resistance in human placental trophoblasts and involvement of the cAMP/PKA/CREB1 signaling pathway in these effects. METHODS: An insulin resistance cell model of human trophoblasts was established. An SHBG‐overexpression plasmid was transfected into these cells, and the expression of glucose transporter 1 (GLUT1), CREB and p‐CREB was detected and analyzed in normal cells, model cells and all groups of transfected cells by real‐time PCR and western blotting; cAMP, PKA, glucose consumption and pyruvic acid levels were also detected. RESULTS: Among the four groups, there was no significant difference in the expression of CREB mRNA or GLUT1 mRNA (P > 0.05); however, CREB, p‐CREB, GLUT1 protein, cAMP and PKA showed low expression (P < 0.05) and cell glucose consumption and pyruvate production were decreased (P < 0.05) in the model group, compared to the normal group. SHBG overexpression in insulin‐resistant cells partially increased the levels of p‐CREB, GLUT1, cAMP and PKA (P < 0.05). Intracellular glucose consumption and pyruvate production were nearly restored to the levels observed in cells from the normal group. CONCLUSION: Sex hormone‐binding globulin regulates GLUT1 expression via the cAMP/PKA/CREB1 pathway and affects glucose transport in the placenta, which can induce insulin resistance and gestational diabetes.
format Online
Article
Text
id pubmed-7692910
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher John Wiley & Sons Australia, Ltd
record_format MEDLINE/PubMed
spelling pubmed-76929102020-12-08 Sex hormone‐binding globulin regulates glucose metabolism in human placental trophoblasts via cAMP/PKA/CREB1 Chi, Xinshu Feng, Chong Wang, Xiaoyan Jin, Zhen J Obstet Gynaecol Res Original Articles AIM: This study was conducted to investigate the effects of sex hormone‐binding globulin (SHBG) on glucose metabolism and insulin resistance in human placental trophoblasts and involvement of the cAMP/PKA/CREB1 signaling pathway in these effects. METHODS: An insulin resistance cell model of human trophoblasts was established. An SHBG‐overexpression plasmid was transfected into these cells, and the expression of glucose transporter 1 (GLUT1), CREB and p‐CREB was detected and analyzed in normal cells, model cells and all groups of transfected cells by real‐time PCR and western blotting; cAMP, PKA, glucose consumption and pyruvic acid levels were also detected. RESULTS: Among the four groups, there was no significant difference in the expression of CREB mRNA or GLUT1 mRNA (P > 0.05); however, CREB, p‐CREB, GLUT1 protein, cAMP and PKA showed low expression (P < 0.05) and cell glucose consumption and pyruvate production were decreased (P < 0.05) in the model group, compared to the normal group. SHBG overexpression in insulin‐resistant cells partially increased the levels of p‐CREB, GLUT1, cAMP and PKA (P < 0.05). Intracellular glucose consumption and pyruvate production were nearly restored to the levels observed in cells from the normal group. CONCLUSION: Sex hormone‐binding globulin regulates GLUT1 expression via the cAMP/PKA/CREB1 pathway and affects glucose transport in the placenta, which can induce insulin resistance and gestational diabetes. John Wiley & Sons Australia, Ltd 2020-08-23 2020-11 /pmc/articles/PMC7692910/ /pubmed/32830408 http://dx.doi.org/10.1111/jog.14429 Text en © 2020 The Authors. Journal of Obstetrics and Gynaecology Research published by John Wiley & Sons Australia, Ltd on behalf of Japan Society of Obstetrics and Gynecology. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Original Articles
Chi, Xinshu
Feng, Chong
Wang, Xiaoyan
Jin, Zhen
Sex hormone‐binding globulin regulates glucose metabolism in human placental trophoblasts via cAMP/PKA/CREB1
title Sex hormone‐binding globulin regulates glucose metabolism in human placental trophoblasts via cAMP/PKA/CREB1
title_full Sex hormone‐binding globulin regulates glucose metabolism in human placental trophoblasts via cAMP/PKA/CREB1
title_fullStr Sex hormone‐binding globulin regulates glucose metabolism in human placental trophoblasts via cAMP/PKA/CREB1
title_full_unstemmed Sex hormone‐binding globulin regulates glucose metabolism in human placental trophoblasts via cAMP/PKA/CREB1
title_short Sex hormone‐binding globulin regulates glucose metabolism in human placental trophoblasts via cAMP/PKA/CREB1
title_sort sex hormone‐binding globulin regulates glucose metabolism in human placental trophoblasts via camp/pka/creb1
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7692910/
https://www.ncbi.nlm.nih.gov/pubmed/32830408
http://dx.doi.org/10.1111/jog.14429
work_keys_str_mv AT chixinshu sexhormonebindingglobulinregulatesglucosemetabolisminhumanplacentaltrophoblastsviacamppkacreb1
AT fengchong sexhormonebindingglobulinregulatesglucosemetabolisminhumanplacentaltrophoblastsviacamppkacreb1
AT wangxiaoyan sexhormonebindingglobulinregulatesglucosemetabolisminhumanplacentaltrophoblastsviacamppkacreb1
AT jinzhen sexhormonebindingglobulinregulatesglucosemetabolisminhumanplacentaltrophoblastsviacamppkacreb1