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Cystic Echinococcosis: Clinical, Immunological, and Biomolecular Evaluation of Patients from Sardinia (Italy)

Cystic echinococcosis (CE), a zoonotic disease caused by the larval stage of the tapeworm Echinococcus granulosus sensu lato (s.l.), is a worldwide public health problem. Echinococcus granulosus sensu stricto (s.s.), associated with G1 and G3 genotypes, is endemic with high prevalence in the Mediter...

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Autores principales: Santucciu, Cinzia, Bonelli, Piero, Peruzzu, Angela, Fancellu, Alessandro, Marras, Vincenzo, Carta, Antonello, Mastrandrea, Scilla, Bagella, Giorgio, Piseddu, Toni, Profili, Stefano, Porcu, Alberto, Masala, Giovanna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7693143/
https://www.ncbi.nlm.nih.gov/pubmed/33143032
http://dx.doi.org/10.3390/pathogens9110907
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author Santucciu, Cinzia
Bonelli, Piero
Peruzzu, Angela
Fancellu, Alessandro
Marras, Vincenzo
Carta, Antonello
Mastrandrea, Scilla
Bagella, Giorgio
Piseddu, Toni
Profili, Stefano
Porcu, Alberto
Masala, Giovanna
author_facet Santucciu, Cinzia
Bonelli, Piero
Peruzzu, Angela
Fancellu, Alessandro
Marras, Vincenzo
Carta, Antonello
Mastrandrea, Scilla
Bagella, Giorgio
Piseddu, Toni
Profili, Stefano
Porcu, Alberto
Masala, Giovanna
author_sort Santucciu, Cinzia
collection PubMed
description Cystic echinococcosis (CE), a zoonotic disease caused by the larval stage of the tapeworm Echinococcus granulosus sensu lato (s.l.), is a worldwide public health problem. Echinococcus granulosus sensu stricto (s.s.), associated with G1 and G3 genotypes, is endemic with high prevalence in the Mediterranean basin. The parasite’s life cycle comprises definitive hosts (canids) and intermediate hosts (ruminants) and can occasionally involve humans. The main aim of this research was to confirm the diagnosis of 13 patients suspected of CE who presented different complications and needed the surgical removal of the cysts. We also wanted to understand and clarify more the diagnosis of echinococcosis in humans. For this purpose, the patients first underwent cyst evaluation by ultrasound (US), immunological analysis, and then total pericystectomy, followed by parasitological, histopathological, and molecular biology examinations of the cysts. US stadiated one CE1, one CE2, eight CE3b, one CE4, and two CE5; immunology evidenced nine positives; histopathology confirmed 11 CE cysts, of which 8 fertile presenting protoscoleces were identified as E. granulosus s.s. by molecular biology, genotyped as three G1 and four G3 by neighbor-joining (NJ) phylogenetic tree. In conclusion, the results showed that 11 patients were affected by E. granulosus s.s. G1 orG3, and 2 cystic neoformations were of non-parasitic origin.
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spelling pubmed-76931432020-11-28 Cystic Echinococcosis: Clinical, Immunological, and Biomolecular Evaluation of Patients from Sardinia (Italy) Santucciu, Cinzia Bonelli, Piero Peruzzu, Angela Fancellu, Alessandro Marras, Vincenzo Carta, Antonello Mastrandrea, Scilla Bagella, Giorgio Piseddu, Toni Profili, Stefano Porcu, Alberto Masala, Giovanna Pathogens Article Cystic echinococcosis (CE), a zoonotic disease caused by the larval stage of the tapeworm Echinococcus granulosus sensu lato (s.l.), is a worldwide public health problem. Echinococcus granulosus sensu stricto (s.s.), associated with G1 and G3 genotypes, is endemic with high prevalence in the Mediterranean basin. The parasite’s life cycle comprises definitive hosts (canids) and intermediate hosts (ruminants) and can occasionally involve humans. The main aim of this research was to confirm the diagnosis of 13 patients suspected of CE who presented different complications and needed the surgical removal of the cysts. We also wanted to understand and clarify more the diagnosis of echinococcosis in humans. For this purpose, the patients first underwent cyst evaluation by ultrasound (US), immunological analysis, and then total pericystectomy, followed by parasitological, histopathological, and molecular biology examinations of the cysts. US stadiated one CE1, one CE2, eight CE3b, one CE4, and two CE5; immunology evidenced nine positives; histopathology confirmed 11 CE cysts, of which 8 fertile presenting protoscoleces were identified as E. granulosus s.s. by molecular biology, genotyped as three G1 and four G3 by neighbor-joining (NJ) phylogenetic tree. In conclusion, the results showed that 11 patients were affected by E. granulosus s.s. G1 orG3, and 2 cystic neoformations were of non-parasitic origin. MDPI 2020-10-30 /pmc/articles/PMC7693143/ /pubmed/33143032 http://dx.doi.org/10.3390/pathogens9110907 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Santucciu, Cinzia
Bonelli, Piero
Peruzzu, Angela
Fancellu, Alessandro
Marras, Vincenzo
Carta, Antonello
Mastrandrea, Scilla
Bagella, Giorgio
Piseddu, Toni
Profili, Stefano
Porcu, Alberto
Masala, Giovanna
Cystic Echinococcosis: Clinical, Immunological, and Biomolecular Evaluation of Patients from Sardinia (Italy)
title Cystic Echinococcosis: Clinical, Immunological, and Biomolecular Evaluation of Patients from Sardinia (Italy)
title_full Cystic Echinococcosis: Clinical, Immunological, and Biomolecular Evaluation of Patients from Sardinia (Italy)
title_fullStr Cystic Echinococcosis: Clinical, Immunological, and Biomolecular Evaluation of Patients from Sardinia (Italy)
title_full_unstemmed Cystic Echinococcosis: Clinical, Immunological, and Biomolecular Evaluation of Patients from Sardinia (Italy)
title_short Cystic Echinococcosis: Clinical, Immunological, and Biomolecular Evaluation of Patients from Sardinia (Italy)
title_sort cystic echinococcosis: clinical, immunological, and biomolecular evaluation of patients from sardinia (italy)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7693143/
https://www.ncbi.nlm.nih.gov/pubmed/33143032
http://dx.doi.org/10.3390/pathogens9110907
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