Enhancing Robustness of Sortase A by Loop Engineering and Backbone Cyclization

Staphylococcus aureus sortase A (SaSrtA) is widely used for site‐specific protein modifications, but it lacks the robustness for performing bioconjugation reactions at elevated temperatures or in presence of denaturing agents. Loop engineering and subsequent head‐to‐tail backbone cyclization of SaSr...

Descripción completa

Detalles Bibliográficos
Autores principales: Zou, Zhi, Mate, Diana M., Nöth, Maximilian, Jakob, Felix, Schwaneberg, Ulrich
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Communications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7693181/
https://www.ncbi.nlm.nih.gov/pubmed/32649777
http://dx.doi.org/10.1002/chem.202002740

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7693181/
https://www.ncbi.nlm.nih.gov/pubmed/32649777
http://dx.doi.org/10.1002/chem.202002740

Ejemplares similares