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Enhancing Robustness of Sortase A by Loop Engineering and Backbone Cyclization
Staphylococcus aureus sortase A (SaSrtA) is widely used for site‐specific protein modifications, but it lacks the robustness for performing bioconjugation reactions at elevated temperatures or in presence of denaturing agents. Loop engineering and subsequent head‐to‐tail backbone cyclization of SaSr...
Autores principales: | Zou, Zhi, Mate, Diana M., Nöth, Maximilian, Jakob, Felix, Schwaneberg, Ulrich |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7693181/ https://www.ncbi.nlm.nih.gov/pubmed/32649777 http://dx.doi.org/10.1002/chem.202002740 |
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