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In Cellulo Protein Semi‐Synthesis from Endogenous and Exogenous Fragments Using the Ultra‐Fast Split Gp41‐1 Intein

Protein semi‐synthesis inside live cells from exogenous and endogenous parts offers unique possibilities for studying proteins in their native context. Split‐intein‐mediated protein trans‐splicing is predestined for such endeavors and has seen some successes, but a much larger variety of established...

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Detalles Bibliográficos
Autores principales: Bhagawati, Maniraj, Hoffmann, Simon, Höffgen, Katharina S., Piehler, Jacob, Busch, Karin B., Mootz, Henning D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7693240/
https://www.ncbi.nlm.nih.gov/pubmed/32777124
http://dx.doi.org/10.1002/anie.202006822