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Antagonistic effects of activin A and TNF-α on the activation of L929 fibroblast cells via Smad3-independent signaling
Fibroblasts play an important role in inflammation and tissue fibrosis. Both activin A and TNF-α can activate immune cells, however, the roles and relationship of them in activating fibroblasts in inflammation remain unclear. Here, this study revealed that TNF-α promoted the release of NO and IL-6 b...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7693280/ https://www.ncbi.nlm.nih.gov/pubmed/33244088 http://dx.doi.org/10.1038/s41598-020-77783-8 |
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author | Jiang, Lingling Liu, Boyang Qi, Yan Zhu, Linru Cui, Xueling Liu, Zhonghui |
author_facet | Jiang, Lingling Liu, Boyang Qi, Yan Zhu, Linru Cui, Xueling Liu, Zhonghui |
author_sort | Jiang, Lingling |
collection | PubMed |
description | Fibroblasts play an important role in inflammation and tissue fibrosis. Both activin A and TNF-α can activate immune cells, however, the roles and relationship of them in activating fibroblasts in inflammation remain unclear. Here, this study revealed that TNF-α promoted the release of NO and IL-6 by L929 fibroblast cells, but co-treatment with activin A attenuated these effects. In contrast, activin A induced cell migration and increased the production of tissue fibrosis-related TGF-β1 and fibronectin, while TNF-α inhibited these function changes of L929 cells induced by activin A. Moreover, this study revealed that activin A and TNF-α regulated the activities of L929 cells via ERK1/2/MAPK pathway, rather than Smad3-dependent signaling pathway. Taken together, these data indicate that activin A and TNF-α exert mutually antagonistic effects on regulating fibroblasts activities, and the balance between their action may determine the process and outcome of fibroblasts-mediated inflammation. |
format | Online Article Text |
id | pubmed-7693280 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-76932802020-11-30 Antagonistic effects of activin A and TNF-α on the activation of L929 fibroblast cells via Smad3-independent signaling Jiang, Lingling Liu, Boyang Qi, Yan Zhu, Linru Cui, Xueling Liu, Zhonghui Sci Rep Article Fibroblasts play an important role in inflammation and tissue fibrosis. Both activin A and TNF-α can activate immune cells, however, the roles and relationship of them in activating fibroblasts in inflammation remain unclear. Here, this study revealed that TNF-α promoted the release of NO and IL-6 by L929 fibroblast cells, but co-treatment with activin A attenuated these effects. In contrast, activin A induced cell migration and increased the production of tissue fibrosis-related TGF-β1 and fibronectin, while TNF-α inhibited these function changes of L929 cells induced by activin A. Moreover, this study revealed that activin A and TNF-α regulated the activities of L929 cells via ERK1/2/MAPK pathway, rather than Smad3-dependent signaling pathway. Taken together, these data indicate that activin A and TNF-α exert mutually antagonistic effects on regulating fibroblasts activities, and the balance between their action may determine the process and outcome of fibroblasts-mediated inflammation. Nature Publishing Group UK 2020-11-26 /pmc/articles/PMC7693280/ /pubmed/33244088 http://dx.doi.org/10.1038/s41598-020-77783-8 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Jiang, Lingling Liu, Boyang Qi, Yan Zhu, Linru Cui, Xueling Liu, Zhonghui Antagonistic effects of activin A and TNF-α on the activation of L929 fibroblast cells via Smad3-independent signaling |
title | Antagonistic effects of activin A and TNF-α on the activation of L929 fibroblast cells via Smad3-independent signaling |
title_full | Antagonistic effects of activin A and TNF-α on the activation of L929 fibroblast cells via Smad3-independent signaling |
title_fullStr | Antagonistic effects of activin A and TNF-α on the activation of L929 fibroblast cells via Smad3-independent signaling |
title_full_unstemmed | Antagonistic effects of activin A and TNF-α on the activation of L929 fibroblast cells via Smad3-independent signaling |
title_short | Antagonistic effects of activin A and TNF-α on the activation of L929 fibroblast cells via Smad3-independent signaling |
title_sort | antagonistic effects of activin a and tnf-α on the activation of l929 fibroblast cells via smad3-independent signaling |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7693280/ https://www.ncbi.nlm.nih.gov/pubmed/33244088 http://dx.doi.org/10.1038/s41598-020-77783-8 |
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