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Antibacterial effects of platelet-rich fibrin produced by horizontal centrifugation

Platelet-rich fibrin (PRF) has been widely used owing to its ability to stimulate tissue regeneration. To date, few studies have described the antibacterial properties of PRF. Previously, PRF prepared by horizontal centrifugation (H-PRF) was shown to contain more immune cells than leukocyte- and pla...

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Autores principales: Feng, Mengge, Wang, Yulan, Zhang, Peng, Zhao, Qin, Yu, Shimin, Shen, Kailun, Miron, Richard J., Zhang, Yufeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7693325/
https://www.ncbi.nlm.nih.gov/pubmed/33243983
http://dx.doi.org/10.1038/s41368-020-00099-w
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author Feng, Mengge
Wang, Yulan
Zhang, Peng
Zhao, Qin
Yu, Shimin
Shen, Kailun
Miron, Richard J.
Zhang, Yufeng
author_facet Feng, Mengge
Wang, Yulan
Zhang, Peng
Zhao, Qin
Yu, Shimin
Shen, Kailun
Miron, Richard J.
Zhang, Yufeng
author_sort Feng, Mengge
collection PubMed
description Platelet-rich fibrin (PRF) has been widely used owing to its ability to stimulate tissue regeneration. To date, few studies have described the antibacterial properties of PRF. Previously, PRF prepared by horizontal centrifugation (H-PRF) was shown to contain more immune cells than leukocyte- and platelet-rich fibrin (L-PRF). This study aimed to compare the antimicrobial effects of PRFs against Staphylococcus aureus and Escherichia coli in vitro and to determine whether the antibacterial effects correlated with the number of immune cells. Blood samples were obtained from eight healthy donors to prepare L-PRF and H-PRF. The sizes and weights of L-PRF and H-PRF were first evaluated, and their antibacterial effects against S. aureus and E. coli were then tested in vitro using the inhibition ring and plate-counting test methods. Flow-cytometric analysis of the cell components of L-PRF and H-PRF was also performed. No significant differences in size or weight were observed between the L-PRF and H-PRF groups. The H-PRF group contained more leukocytes than the L-PRF group. While both PRFs had notable antimicrobial activity against S. aureus and E. coli, H-PRF demonstrated a significantly better antibacterial effect than L-PRF. Furthermore, the antimicrobial ability of the PRF solid was less efficient than that of wet PRF. In conclusion, H-PRF exhibited better antibacterial activity than L-PRF, which might have been attributed to having more immune cells.
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spelling pubmed-76933252020-11-30 Antibacterial effects of platelet-rich fibrin produced by horizontal centrifugation Feng, Mengge Wang, Yulan Zhang, Peng Zhao, Qin Yu, Shimin Shen, Kailun Miron, Richard J. Zhang, Yufeng Int J Oral Sci Article Platelet-rich fibrin (PRF) has been widely used owing to its ability to stimulate tissue regeneration. To date, few studies have described the antibacterial properties of PRF. Previously, PRF prepared by horizontal centrifugation (H-PRF) was shown to contain more immune cells than leukocyte- and platelet-rich fibrin (L-PRF). This study aimed to compare the antimicrobial effects of PRFs against Staphylococcus aureus and Escherichia coli in vitro and to determine whether the antibacterial effects correlated with the number of immune cells. Blood samples were obtained from eight healthy donors to prepare L-PRF and H-PRF. The sizes and weights of L-PRF and H-PRF were first evaluated, and their antibacterial effects against S. aureus and E. coli were then tested in vitro using the inhibition ring and plate-counting test methods. Flow-cytometric analysis of the cell components of L-PRF and H-PRF was also performed. No significant differences in size or weight were observed between the L-PRF and H-PRF groups. The H-PRF group contained more leukocytes than the L-PRF group. While both PRFs had notable antimicrobial activity against S. aureus and E. coli, H-PRF demonstrated a significantly better antibacterial effect than L-PRF. Furthermore, the antimicrobial ability of the PRF solid was less efficient than that of wet PRF. In conclusion, H-PRF exhibited better antibacterial activity than L-PRF, which might have been attributed to having more immune cells. Nature Publishing Group UK 2020-11-26 /pmc/articles/PMC7693325/ /pubmed/33243983 http://dx.doi.org/10.1038/s41368-020-00099-w Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Feng, Mengge
Wang, Yulan
Zhang, Peng
Zhao, Qin
Yu, Shimin
Shen, Kailun
Miron, Richard J.
Zhang, Yufeng
Antibacterial effects of platelet-rich fibrin produced by horizontal centrifugation
title Antibacterial effects of platelet-rich fibrin produced by horizontal centrifugation
title_full Antibacterial effects of platelet-rich fibrin produced by horizontal centrifugation
title_fullStr Antibacterial effects of platelet-rich fibrin produced by horizontal centrifugation
title_full_unstemmed Antibacterial effects of platelet-rich fibrin produced by horizontal centrifugation
title_short Antibacterial effects of platelet-rich fibrin produced by horizontal centrifugation
title_sort antibacterial effects of platelet-rich fibrin produced by horizontal centrifugation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7693325/
https://www.ncbi.nlm.nih.gov/pubmed/33243983
http://dx.doi.org/10.1038/s41368-020-00099-w
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