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High-speed volumetric two-photon fluorescence imaging of neurovascular dynamics

Understanding the structure and function of vasculature in the brain requires us to monitor distributed hemodynamics at high spatial and temporal resolution in three-dimensional (3D) volumes in vivo. Currently, a volumetric vasculature imaging method with sub-capillary spatial resolution and blood f...

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Autores principales: Fan, Jiang Lan, Rivera, Jose A., Sun, Wei, Peterson, John, Haeberle, Henry, Rubin, Sam, Ji, Na
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7693336/
https://www.ncbi.nlm.nih.gov/pubmed/33243995
http://dx.doi.org/10.1038/s41467-020-19851-1
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author Fan, Jiang Lan
Rivera, Jose A.
Sun, Wei
Peterson, John
Haeberle, Henry
Rubin, Sam
Ji, Na
author_facet Fan, Jiang Lan
Rivera, Jose A.
Sun, Wei
Peterson, John
Haeberle, Henry
Rubin, Sam
Ji, Na
author_sort Fan, Jiang Lan
collection PubMed
description Understanding the structure and function of vasculature in the brain requires us to monitor distributed hemodynamics at high spatial and temporal resolution in three-dimensional (3D) volumes in vivo. Currently, a volumetric vasculature imaging method with sub-capillary spatial resolution and blood flow-resolving speed is lacking. Here, using two-photon laser scanning microscopy (TPLSM) with an axially extended Bessel focus, we capture volumetric hemodynamics in the awake mouse brain at a spatiotemporal resolution sufficient for measuring capillary size and blood flow. With Bessel TPLSM, the fluorescence signal of a vessel becomes proportional to its size, which enables convenient intensity-based analysis of vessel dilation and constriction dynamics in large volumes. We observe entrainment of vasodilation and vasoconstriction with pupil diameter and measure 3D blood flow at 99 volumes/second. Demonstrating high-throughput monitoring of hemodynamics in the awake brain, we expect Bessel TPLSM to make broad impacts on neurovasculature research.
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spelling pubmed-76933362020-12-03 High-speed volumetric two-photon fluorescence imaging of neurovascular dynamics Fan, Jiang Lan Rivera, Jose A. Sun, Wei Peterson, John Haeberle, Henry Rubin, Sam Ji, Na Nat Commun Article Understanding the structure and function of vasculature in the brain requires us to monitor distributed hemodynamics at high spatial and temporal resolution in three-dimensional (3D) volumes in vivo. Currently, a volumetric vasculature imaging method with sub-capillary spatial resolution and blood flow-resolving speed is lacking. Here, using two-photon laser scanning microscopy (TPLSM) with an axially extended Bessel focus, we capture volumetric hemodynamics in the awake mouse brain at a spatiotemporal resolution sufficient for measuring capillary size and blood flow. With Bessel TPLSM, the fluorescence signal of a vessel becomes proportional to its size, which enables convenient intensity-based analysis of vessel dilation and constriction dynamics in large volumes. We observe entrainment of vasodilation and vasoconstriction with pupil diameter and measure 3D blood flow at 99 volumes/second. Demonstrating high-throughput monitoring of hemodynamics in the awake brain, we expect Bessel TPLSM to make broad impacts on neurovasculature research. Nature Publishing Group UK 2020-11-26 /pmc/articles/PMC7693336/ /pubmed/33243995 http://dx.doi.org/10.1038/s41467-020-19851-1 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Fan, Jiang Lan
Rivera, Jose A.
Sun, Wei
Peterson, John
Haeberle, Henry
Rubin, Sam
Ji, Na
High-speed volumetric two-photon fluorescence imaging of neurovascular dynamics
title High-speed volumetric two-photon fluorescence imaging of neurovascular dynamics
title_full High-speed volumetric two-photon fluorescence imaging of neurovascular dynamics
title_fullStr High-speed volumetric two-photon fluorescence imaging of neurovascular dynamics
title_full_unstemmed High-speed volumetric two-photon fluorescence imaging of neurovascular dynamics
title_short High-speed volumetric two-photon fluorescence imaging of neurovascular dynamics
title_sort high-speed volumetric two-photon fluorescence imaging of neurovascular dynamics
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7693336/
https://www.ncbi.nlm.nih.gov/pubmed/33243995
http://dx.doi.org/10.1038/s41467-020-19851-1
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