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In-House Developed ELISA Indicates High Prevalence of Anti-Echinococcus granulosus IgG in Sheep Population—An Update from Pakistan

Cystic echinococcosis (CE) is a World Health Organization (WHO)-listed neglected tropical farm economy jeopardizing and public health concern disease. This study was aimed at furnishing sero-epidemiological baseline data of CE in sheep in Pakistan, where data are non-existent. For this purpose, two...

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Autores principales: Alvi, Mughees Aizaz, Ohiolei, John Asekhaen, Li, Li, Saqib, Muhammad, Hussain, Muhammad Hammad, Tayyab, Muhammad Haleem, Ghafoor, Muzafar, Qamar, Warda, Faridi, Noman Yousaf, Alvi, Anum Aizaz, Fu, Bao-Quan, Yan, Hong-Bin, Jia, Wan-Zhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7693474/
https://www.ncbi.nlm.nih.gov/pubmed/33137933
http://dx.doi.org/10.3390/pathogens9110905
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author Alvi, Mughees Aizaz
Ohiolei, John Asekhaen
Li, Li
Saqib, Muhammad
Hussain, Muhammad Hammad
Tayyab, Muhammad Haleem
Ghafoor, Muzafar
Qamar, Warda
Faridi, Noman Yousaf
Alvi, Anum Aizaz
Fu, Bao-Quan
Yan, Hong-Bin
Jia, Wan-Zhong
author_facet Alvi, Mughees Aizaz
Ohiolei, John Asekhaen
Li, Li
Saqib, Muhammad
Hussain, Muhammad Hammad
Tayyab, Muhammad Haleem
Ghafoor, Muzafar
Qamar, Warda
Faridi, Noman Yousaf
Alvi, Anum Aizaz
Fu, Bao-Quan
Yan, Hong-Bin
Jia, Wan-Zhong
author_sort Alvi, Mughees Aizaz
collection PubMed
description Cystic echinococcosis (CE) is a World Health Organization (WHO)-listed neglected tropical farm economy jeopardizing and public health concern disease. This study was aimed at furnishing sero-epidemiological baseline data of CE in sheep in Pakistan, where data are non-existent. For this purpose, two sheep-rich provinces of Pakistan were selected, and 728 sheep sera were collected using probability proportional to size (PPS) statistical technique. Epidemiological information was recorded on a questionnaire for the estimation of potential risk factors. The serum samples were analyzed for IgG antibodies against Echinococcus granulosus using an in-house-developed EgAgB-based ELISA kit. The overall seroprevalence recorded was 21.98% (160/728) in the tested sheep, suggesting higher seropositivity in sheep from Punjab (23.73%) as compared to Khyber Pakhtunkhwa (KPK) (19.04%). The overall apparent prevalence observed by this ELISA method was almost similar to the calculated true prevalence (21.77%). Prevalence was significantly different (p < 0.05) among sheep from different districts. Higher prevalence was found in females (22.54%, OR 1.41), age group > 5 years (29.66%, OR 1.64), crossbreeds (42.85%, OR 2.70), and sheep with pasture access (25.96%, OR 3.06). Being in age group > 5 years and having pasture access were the factors significantly associated with seropositivity (p < 0.05). This study provides serological evidence of E. granulosus infection in sheep and can be used as a model for ante-mortem screening of the sheep globally.
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spelling pubmed-76934742020-11-28 In-House Developed ELISA Indicates High Prevalence of Anti-Echinococcus granulosus IgG in Sheep Population—An Update from Pakistan Alvi, Mughees Aizaz Ohiolei, John Asekhaen Li, Li Saqib, Muhammad Hussain, Muhammad Hammad Tayyab, Muhammad Haleem Ghafoor, Muzafar Qamar, Warda Faridi, Noman Yousaf Alvi, Anum Aizaz Fu, Bao-Quan Yan, Hong-Bin Jia, Wan-Zhong Pathogens Brief Report Cystic echinococcosis (CE) is a World Health Organization (WHO)-listed neglected tropical farm economy jeopardizing and public health concern disease. This study was aimed at furnishing sero-epidemiological baseline data of CE in sheep in Pakistan, where data are non-existent. For this purpose, two sheep-rich provinces of Pakistan were selected, and 728 sheep sera were collected using probability proportional to size (PPS) statistical technique. Epidemiological information was recorded on a questionnaire for the estimation of potential risk factors. The serum samples were analyzed for IgG antibodies against Echinococcus granulosus using an in-house-developed EgAgB-based ELISA kit. The overall seroprevalence recorded was 21.98% (160/728) in the tested sheep, suggesting higher seropositivity in sheep from Punjab (23.73%) as compared to Khyber Pakhtunkhwa (KPK) (19.04%). The overall apparent prevalence observed by this ELISA method was almost similar to the calculated true prevalence (21.77%). Prevalence was significantly different (p < 0.05) among sheep from different districts. Higher prevalence was found in females (22.54%, OR 1.41), age group > 5 years (29.66%, OR 1.64), crossbreeds (42.85%, OR 2.70), and sheep with pasture access (25.96%, OR 3.06). Being in age group > 5 years and having pasture access were the factors significantly associated with seropositivity (p < 0.05). This study provides serological evidence of E. granulosus infection in sheep and can be used as a model for ante-mortem screening of the sheep globally. MDPI 2020-10-29 /pmc/articles/PMC7693474/ /pubmed/33137933 http://dx.doi.org/10.3390/pathogens9110905 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Brief Report
Alvi, Mughees Aizaz
Ohiolei, John Asekhaen
Li, Li
Saqib, Muhammad
Hussain, Muhammad Hammad
Tayyab, Muhammad Haleem
Ghafoor, Muzafar
Qamar, Warda
Faridi, Noman Yousaf
Alvi, Anum Aizaz
Fu, Bao-Quan
Yan, Hong-Bin
Jia, Wan-Zhong
In-House Developed ELISA Indicates High Prevalence of Anti-Echinococcus granulosus IgG in Sheep Population—An Update from Pakistan
title In-House Developed ELISA Indicates High Prevalence of Anti-Echinococcus granulosus IgG in Sheep Population—An Update from Pakistan
title_full In-House Developed ELISA Indicates High Prevalence of Anti-Echinococcus granulosus IgG in Sheep Population—An Update from Pakistan
title_fullStr In-House Developed ELISA Indicates High Prevalence of Anti-Echinococcus granulosus IgG in Sheep Population—An Update from Pakistan
title_full_unstemmed In-House Developed ELISA Indicates High Prevalence of Anti-Echinococcus granulosus IgG in Sheep Population—An Update from Pakistan
title_short In-House Developed ELISA Indicates High Prevalence of Anti-Echinococcus granulosus IgG in Sheep Population—An Update from Pakistan
title_sort in-house developed elisa indicates high prevalence of anti-echinococcus granulosus igg in sheep population—an update from pakistan
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7693474/
https://www.ncbi.nlm.nih.gov/pubmed/33137933
http://dx.doi.org/10.3390/pathogens9110905
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