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Secreted Factors from Stem Cells of Human Exfoliated Deciduous Teeth Directly Activate Endothelial Cells to Promote All Processes of Angiogenesis

Diabetes is a major risk factor for atherosclerosis and ischemic vascular diseases. Recently, regenerative medicine is expected to be a novel therapy for ischemic diseases. Our previous studies have reported that transplantation of stem cells promoted therapeutic angiogenesis for diabetic neuropathy...

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Autores principales: Kato, Makoto, Tsunekawa, Shin, Nakamura, Nobuhisa, Miura-Yura, Emiri, Yamada, Yuichiro, Hayashi, Yusuke, Nakai-Shimoda, Hiromi, Asano, Saeko, Hayami, Tomohide, Motegi, Mikio, Asano-Hayami, Emi, Sasajima, Sachiko, Morishita, Yoshiaki, Himeno, Tatsuhito, Kondo, Masaki, Kato, Yoshiro, Izumoto-Akita, Takako, Yamamoto, Akihito, Naruse, Keiko, Nakamura, Jiro, Kamiya, Hideki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7693657/
https://www.ncbi.nlm.nih.gov/pubmed/33142678
http://dx.doi.org/10.3390/cells9112385
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author Kato, Makoto
Tsunekawa, Shin
Nakamura, Nobuhisa
Miura-Yura, Emiri
Yamada, Yuichiro
Hayashi, Yusuke
Nakai-Shimoda, Hiromi
Asano, Saeko
Hayami, Tomohide
Motegi, Mikio
Asano-Hayami, Emi
Sasajima, Sachiko
Morishita, Yoshiaki
Himeno, Tatsuhito
Kondo, Masaki
Kato, Yoshiro
Izumoto-Akita, Takako
Yamamoto, Akihito
Naruse, Keiko
Nakamura, Jiro
Kamiya, Hideki
author_facet Kato, Makoto
Tsunekawa, Shin
Nakamura, Nobuhisa
Miura-Yura, Emiri
Yamada, Yuichiro
Hayashi, Yusuke
Nakai-Shimoda, Hiromi
Asano, Saeko
Hayami, Tomohide
Motegi, Mikio
Asano-Hayami, Emi
Sasajima, Sachiko
Morishita, Yoshiaki
Himeno, Tatsuhito
Kondo, Masaki
Kato, Yoshiro
Izumoto-Akita, Takako
Yamamoto, Akihito
Naruse, Keiko
Nakamura, Jiro
Kamiya, Hideki
author_sort Kato, Makoto
collection PubMed
description Diabetes is a major risk factor for atherosclerosis and ischemic vascular diseases. Recently, regenerative medicine is expected to be a novel therapy for ischemic diseases. Our previous studies have reported that transplantation of stem cells promoted therapeutic angiogenesis for diabetic neuropathy and ischemic vascular disease in a paracrine manner, but the precise mechanism is unclear. Therefore, we examined whether secreted factors from stem cells had direct beneficial effects on endothelial cells to promote angiogenesis. The soluble factors were collected as conditioned medium (CM) 48 h after culturing stem cells from human exfoliated deciduous teeth (SHED) in serum-free DMEM. SHED-CM significantly increased cell viability of human umbilical vein endothelial cells (HUVECs) in MTT assays and accelerated HUVECs migration in wound healing and Boyden chamber assays. In a Matrigel plug assay of mice, the migrated number of primary endothelial cells was markedly increased in the plug containing SHED-CM or SHED suspension. SHED-CM induced complex tubular structures of HUVECs in a tube formation assay. Furthermore, SHED-CM significantly increased neovascularization from the primary rat aorta, indicating that SHED-CM stimulated primary endothelial cells to promote comprehensive angiogenesis processes. The angiogenic effects of SHED-CM were the same or greater than the effective concentration of VEGF. In conclusion, SHED-CM directly stimulates vascular endothelial cells to promote angiogenesis and is promising for future clinical application.
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spelling pubmed-76936572020-11-28 Secreted Factors from Stem Cells of Human Exfoliated Deciduous Teeth Directly Activate Endothelial Cells to Promote All Processes of Angiogenesis Kato, Makoto Tsunekawa, Shin Nakamura, Nobuhisa Miura-Yura, Emiri Yamada, Yuichiro Hayashi, Yusuke Nakai-Shimoda, Hiromi Asano, Saeko Hayami, Tomohide Motegi, Mikio Asano-Hayami, Emi Sasajima, Sachiko Morishita, Yoshiaki Himeno, Tatsuhito Kondo, Masaki Kato, Yoshiro Izumoto-Akita, Takako Yamamoto, Akihito Naruse, Keiko Nakamura, Jiro Kamiya, Hideki Cells Article Diabetes is a major risk factor for atherosclerosis and ischemic vascular diseases. Recently, regenerative medicine is expected to be a novel therapy for ischemic diseases. Our previous studies have reported that transplantation of stem cells promoted therapeutic angiogenesis for diabetic neuropathy and ischemic vascular disease in a paracrine manner, but the precise mechanism is unclear. Therefore, we examined whether secreted factors from stem cells had direct beneficial effects on endothelial cells to promote angiogenesis. The soluble factors were collected as conditioned medium (CM) 48 h after culturing stem cells from human exfoliated deciduous teeth (SHED) in serum-free DMEM. SHED-CM significantly increased cell viability of human umbilical vein endothelial cells (HUVECs) in MTT assays and accelerated HUVECs migration in wound healing and Boyden chamber assays. In a Matrigel plug assay of mice, the migrated number of primary endothelial cells was markedly increased in the plug containing SHED-CM or SHED suspension. SHED-CM induced complex tubular structures of HUVECs in a tube formation assay. Furthermore, SHED-CM significantly increased neovascularization from the primary rat aorta, indicating that SHED-CM stimulated primary endothelial cells to promote comprehensive angiogenesis processes. The angiogenic effects of SHED-CM were the same or greater than the effective concentration of VEGF. In conclusion, SHED-CM directly stimulates vascular endothelial cells to promote angiogenesis and is promising for future clinical application. MDPI 2020-10-31 /pmc/articles/PMC7693657/ /pubmed/33142678 http://dx.doi.org/10.3390/cells9112385 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kato, Makoto
Tsunekawa, Shin
Nakamura, Nobuhisa
Miura-Yura, Emiri
Yamada, Yuichiro
Hayashi, Yusuke
Nakai-Shimoda, Hiromi
Asano, Saeko
Hayami, Tomohide
Motegi, Mikio
Asano-Hayami, Emi
Sasajima, Sachiko
Morishita, Yoshiaki
Himeno, Tatsuhito
Kondo, Masaki
Kato, Yoshiro
Izumoto-Akita, Takako
Yamamoto, Akihito
Naruse, Keiko
Nakamura, Jiro
Kamiya, Hideki
Secreted Factors from Stem Cells of Human Exfoliated Deciduous Teeth Directly Activate Endothelial Cells to Promote All Processes of Angiogenesis
title Secreted Factors from Stem Cells of Human Exfoliated Deciduous Teeth Directly Activate Endothelial Cells to Promote All Processes of Angiogenesis
title_full Secreted Factors from Stem Cells of Human Exfoliated Deciduous Teeth Directly Activate Endothelial Cells to Promote All Processes of Angiogenesis
title_fullStr Secreted Factors from Stem Cells of Human Exfoliated Deciduous Teeth Directly Activate Endothelial Cells to Promote All Processes of Angiogenesis
title_full_unstemmed Secreted Factors from Stem Cells of Human Exfoliated Deciduous Teeth Directly Activate Endothelial Cells to Promote All Processes of Angiogenesis
title_short Secreted Factors from Stem Cells of Human Exfoliated Deciduous Teeth Directly Activate Endothelial Cells to Promote All Processes of Angiogenesis
title_sort secreted factors from stem cells of human exfoliated deciduous teeth directly activate endothelial cells to promote all processes of angiogenesis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7693657/
https://www.ncbi.nlm.nih.gov/pubmed/33142678
http://dx.doi.org/10.3390/cells9112385
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