Cargando…

Concentrated Growth Factors (CGF) Induce Osteogenic Differentiation in Human Bone Marrow Stem Cells

SIMPLE SUMMARY: Osteogenesis is a complex physiologic process that occurs during development as well as during damaged bone regeneration. This process requires several growth factors that act on stem cell populations, including Bone Marrow Stem Cells (BMSC). The present study fits into the research...

Descripción completa

Detalles Bibliográficos
Autores principales: Rochira, Alessio, Siculella, Luisa, Damiano, Fabrizio, Palermo, Andrea, Ferrante, Franco, Carluccio, Maria Annunziata, Calabriso, Nadia, Giannotti, Laura, Stanca, Eleonora
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7693660/
https://www.ncbi.nlm.nih.gov/pubmed/33143015
http://dx.doi.org/10.3390/biology9110370
_version_ 1783614796251267072
author Rochira, Alessio
Siculella, Luisa
Damiano, Fabrizio
Palermo, Andrea
Ferrante, Franco
Carluccio, Maria Annunziata
Calabriso, Nadia
Giannotti, Laura
Stanca, Eleonora
author_facet Rochira, Alessio
Siculella, Luisa
Damiano, Fabrizio
Palermo, Andrea
Ferrante, Franco
Carluccio, Maria Annunziata
Calabriso, Nadia
Giannotti, Laura
Stanca, Eleonora
author_sort Rochira, Alessio
collection PubMed
description SIMPLE SUMMARY: Osteogenesis is a complex physiologic process that occurs during development as well as during damaged bone regeneration. This process requires several growth factors that act on stem cell populations, including Bone Marrow Stem Cells (BMSC). The present study fits into the research field for safe improvement of cell osteogenesis induction. In this context there is a great interest on an autologous and biocompatible blood derived product, named Concentrated Growth Factor (CGF). In particular, the ability of CGF to induce osteogenic differentiation of human BMSC (hBMSC) in vitro was here investigated. The osteogenic differentiation was evaluated measuring typical osteogenic markers such as alkaline phosphatase enzyme activity, matrix mineralization of hBMSC, and expression of some osteogenic-related genes. The results show that CGF alone is able to induce osteogenic differentiation of hBMSC. This finding opens up further, interesting perspectives in the biotechnological use of CGF in the tissue regeneration field. ABSTRACT: Bone regeneration is a complex process regulated by several factors that control overlapping biological processes, coordinating interactions among distinct cell populations. There is a great interest in identifying new strategies for inducing osteogenesis in a safe and efficient manner. Concentrated Growth Factor (CGF) is an autologous blood derived product obtained by centrifugation of venous blood following the procedure set on the Silfradent device. In this study the effects of CGF on osteogenic differentiation of human Bone Marrow Stem Cells (hBMSC) in vitro have been investigated; hBMSC were cultured with CGF or osteogenic medium, for 21 days. The osteogenic differentiation was evaluated measuring alkaline phosphatase (ALP) enzyme activity, matrix mineralization by alizarin red staining and through mRNA and protein quantification of osteogenic differentiation markers by Real-time PCR and Western blotting, respectively. The treatment with CGF stimulated ALP activity and promoted matrix mineralization compared to control and seems to be more effective than osteogenic medium. Also, hBMSC lost mesenchymal markers and showed other osteogenic features. Our study showed for the first time that CGF alone is able to induce osteogenic differentiation in hBMSC. The application of CGF on hBMSC osteoinduction might offer new clinical and biotechnological strategies in the tissue regeneration field.
format Online
Article
Text
id pubmed-7693660
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-76936602020-11-28 Concentrated Growth Factors (CGF) Induce Osteogenic Differentiation in Human Bone Marrow Stem Cells Rochira, Alessio Siculella, Luisa Damiano, Fabrizio Palermo, Andrea Ferrante, Franco Carluccio, Maria Annunziata Calabriso, Nadia Giannotti, Laura Stanca, Eleonora Biology (Basel) Article SIMPLE SUMMARY: Osteogenesis is a complex physiologic process that occurs during development as well as during damaged bone regeneration. This process requires several growth factors that act on stem cell populations, including Bone Marrow Stem Cells (BMSC). The present study fits into the research field for safe improvement of cell osteogenesis induction. In this context there is a great interest on an autologous and biocompatible blood derived product, named Concentrated Growth Factor (CGF). In particular, the ability of CGF to induce osteogenic differentiation of human BMSC (hBMSC) in vitro was here investigated. The osteogenic differentiation was evaluated measuring typical osteogenic markers such as alkaline phosphatase enzyme activity, matrix mineralization of hBMSC, and expression of some osteogenic-related genes. The results show that CGF alone is able to induce osteogenic differentiation of hBMSC. This finding opens up further, interesting perspectives in the biotechnological use of CGF in the tissue regeneration field. ABSTRACT: Bone regeneration is a complex process regulated by several factors that control overlapping biological processes, coordinating interactions among distinct cell populations. There is a great interest in identifying new strategies for inducing osteogenesis in a safe and efficient manner. Concentrated Growth Factor (CGF) is an autologous blood derived product obtained by centrifugation of venous blood following the procedure set on the Silfradent device. In this study the effects of CGF on osteogenic differentiation of human Bone Marrow Stem Cells (hBMSC) in vitro have been investigated; hBMSC were cultured with CGF or osteogenic medium, for 21 days. The osteogenic differentiation was evaluated measuring alkaline phosphatase (ALP) enzyme activity, matrix mineralization by alizarin red staining and through mRNA and protein quantification of osteogenic differentiation markers by Real-time PCR and Western blotting, respectively. The treatment with CGF stimulated ALP activity and promoted matrix mineralization compared to control and seems to be more effective than osteogenic medium. Also, hBMSC lost mesenchymal markers and showed other osteogenic features. Our study showed for the first time that CGF alone is able to induce osteogenic differentiation in hBMSC. The application of CGF on hBMSC osteoinduction might offer new clinical and biotechnological strategies in the tissue regeneration field. MDPI 2020-10-30 /pmc/articles/PMC7693660/ /pubmed/33143015 http://dx.doi.org/10.3390/biology9110370 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Rochira, Alessio
Siculella, Luisa
Damiano, Fabrizio
Palermo, Andrea
Ferrante, Franco
Carluccio, Maria Annunziata
Calabriso, Nadia
Giannotti, Laura
Stanca, Eleonora
Concentrated Growth Factors (CGF) Induce Osteogenic Differentiation in Human Bone Marrow Stem Cells
title Concentrated Growth Factors (CGF) Induce Osteogenic Differentiation in Human Bone Marrow Stem Cells
title_full Concentrated Growth Factors (CGF) Induce Osteogenic Differentiation in Human Bone Marrow Stem Cells
title_fullStr Concentrated Growth Factors (CGF) Induce Osteogenic Differentiation in Human Bone Marrow Stem Cells
title_full_unstemmed Concentrated Growth Factors (CGF) Induce Osteogenic Differentiation in Human Bone Marrow Stem Cells
title_short Concentrated Growth Factors (CGF) Induce Osteogenic Differentiation in Human Bone Marrow Stem Cells
title_sort concentrated growth factors (cgf) induce osteogenic differentiation in human bone marrow stem cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7693660/
https://www.ncbi.nlm.nih.gov/pubmed/33143015
http://dx.doi.org/10.3390/biology9110370
work_keys_str_mv AT rochiraalessio concentratedgrowthfactorscgfinduceosteogenicdifferentiationinhumanbonemarrowstemcells
AT siculellaluisa concentratedgrowthfactorscgfinduceosteogenicdifferentiationinhumanbonemarrowstemcells
AT damianofabrizio concentratedgrowthfactorscgfinduceosteogenicdifferentiationinhumanbonemarrowstemcells
AT palermoandrea concentratedgrowthfactorscgfinduceosteogenicdifferentiationinhumanbonemarrowstemcells
AT ferrantefranco concentratedgrowthfactorscgfinduceosteogenicdifferentiationinhumanbonemarrowstemcells
AT carlucciomariaannunziata concentratedgrowthfactorscgfinduceosteogenicdifferentiationinhumanbonemarrowstemcells
AT calabrisonadia concentratedgrowthfactorscgfinduceosteogenicdifferentiationinhumanbonemarrowstemcells
AT giannottilaura concentratedgrowthfactorscgfinduceosteogenicdifferentiationinhumanbonemarrowstemcells
AT stancaeleonora concentratedgrowthfactorscgfinduceosteogenicdifferentiationinhumanbonemarrowstemcells