Use of Undigested NDF for Estimation of Diet Digestibility in Growing Pigs

SIMPLE SUMMARY: Knowledge of diet digestibility in animals is essential to reduce feed costs and the amount of undigested nutrients excreted in the manure. Diet digestibility can be evaluated via in vivo, in situ (i.e., in the rumen of cannulated animals), and in vitro methods. The in vivo by total faecal collection methods and the in situ methods are expensive and ethically questionable. The in vitro methods are cheaper but also less accurate. This work aimed to verify whether the in vivo method with undigested neutral detergent fibre (uNDF), which is commonly used in ruminants as an internal marker, could be adapted toward growing pigs. Dry matter, organic matter, and neutral detergent fibre digestibilities estimated with the uNDF were compared with in vivo values determined by total faecal collection in a previous study. The effects of pre-treating samples with the neutral detergent solution and adding α-amylase were also tested to improve the repeatability and accuracy of the results. It was concluded that the estimation of diet digestibility with pre-treated uNDF as an internal marker in growing pigs could be an alternative to the total faecal collection method. ABSTRACT: Undigested neutral detergent fibre (uNDF) is commonly used as an internal marker for the estimation of diet digestibility in ruminants. This work aimed to verify (i) whether the in vivo method with uNDF could be used to evaluate diet digestibility in growing pigs, and (ii) whether pre-treating the samples with neutral detergent solution (NDS) and α-amylase improves the accuracy of the estimates. Samples from a previously published work of two diets with known in vivo digestibility values estimated by the total faecal collection method and 16 individual samples of faeces were used. For each sample, four Ankom F57 bags were weighed. Before the incubation, two F57 bags were pre-treated with NDS and α-amylase. All the samples were incubated for 240 h in the Ankom Daisy(II) incubator and then analysed for their uNDF contents. Dry matter, organic matter, and neutral detergent fibre digestibilities were estimated using the uNDF contents, and the results were compared with those of the former study. The digestibility values obtained using the uNDF method with pre-treatment were not statistically different from those determined with the total faecal collection. On the contrary, the uNDF method without the pre-treatment could not satisfactorily predict the digestibilities of pig diets.