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Effects of Heavy Ion Particle Irradiation on Spore Germination of Bacillus spp. from Extremely Hot and Cold Environments

Extremophiles are optimal models in experimentally addressing questions about the effects of cosmic radiation on biological systems. The resistance to high charge energy (HZE) particles, and helium (He) ions and iron (Fe) ions (LET at 2.2 and 200 keV/µm, respectively, until 1000 Gy), of spores from...

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Autores principales: Zammuto, Vincenzo, Rizzo, Maria G., De Plano, Laura M., Franco, Domenico, Guglielmino, Salvatore, Caccamo, Maria T., Magazù, Salvatore, Fujimori, Akira, Giudice, Angelina Lo, Guglielmin, Mauro, McAlpin, Kevin Roderick, Moeller, Ralf, Gugliandolo, Concetta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7693761/
https://www.ncbi.nlm.nih.gov/pubmed/33143156
http://dx.doi.org/10.3390/life10110264
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author Zammuto, Vincenzo
Rizzo, Maria G.
De Plano, Laura M.
Franco, Domenico
Guglielmino, Salvatore
Caccamo, Maria T.
Magazù, Salvatore
Fujimori, Akira
Giudice, Angelina Lo
Guglielmin, Mauro
McAlpin, Kevin Roderick
Moeller, Ralf
Gugliandolo, Concetta
author_facet Zammuto, Vincenzo
Rizzo, Maria G.
De Plano, Laura M.
Franco, Domenico
Guglielmino, Salvatore
Caccamo, Maria T.
Magazù, Salvatore
Fujimori, Akira
Giudice, Angelina Lo
Guglielmin, Mauro
McAlpin, Kevin Roderick
Moeller, Ralf
Gugliandolo, Concetta
author_sort Zammuto, Vincenzo
collection PubMed
description Extremophiles are optimal models in experimentally addressing questions about the effects of cosmic radiation on biological systems. The resistance to high charge energy (HZE) particles, and helium (He) ions and iron (Fe) ions (LET at 2.2 and 200 keV/µm, respectively, until 1000 Gy), of spores from two thermophiles, Bacillus horneckiae SBP3 and Bacillus licheniformis T14, and two psychrotolerants, Bacillus sp. A34 and A43, was investigated. Spores survived He irradiation better, whereas they were more sensitive to Fe irradiation (until 500 Gy), with spores from thermophiles being more resistant to irradiations than psychrotolerants. The survived spores showed different germination kinetics, depending on the type/dose of irradiation and the germinant used. After exposure to He 1000 Gy, D-glucose increased the lag time of thermophilic spores and induced germination of psychrotolerants, whereas L-alanine and L-valine increased the germination efficiency, except alanine for A43. FTIR spectra showed important modifications to the structural components of spores after Fe irradiation at 250 Gy, which could explain the block in spore germination, whereas minor changes were observed after He radiation that could be related to the increased permeability of the inner membranes and alterations of receptor complex structures. Our results give new insights on HZE resistance of extremophiles that are useful in different contexts, including astrobiology.
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spelling pubmed-76937612020-11-28 Effects of Heavy Ion Particle Irradiation on Spore Germination of Bacillus spp. from Extremely Hot and Cold Environments Zammuto, Vincenzo Rizzo, Maria G. De Plano, Laura M. Franco, Domenico Guglielmino, Salvatore Caccamo, Maria T. Magazù, Salvatore Fujimori, Akira Giudice, Angelina Lo Guglielmin, Mauro McAlpin, Kevin Roderick Moeller, Ralf Gugliandolo, Concetta Life (Basel) Article Extremophiles are optimal models in experimentally addressing questions about the effects of cosmic radiation on biological systems. The resistance to high charge energy (HZE) particles, and helium (He) ions and iron (Fe) ions (LET at 2.2 and 200 keV/µm, respectively, until 1000 Gy), of spores from two thermophiles, Bacillus horneckiae SBP3 and Bacillus licheniformis T14, and two psychrotolerants, Bacillus sp. A34 and A43, was investigated. Spores survived He irradiation better, whereas they were more sensitive to Fe irradiation (until 500 Gy), with spores from thermophiles being more resistant to irradiations than psychrotolerants. The survived spores showed different germination kinetics, depending on the type/dose of irradiation and the germinant used. After exposure to He 1000 Gy, D-glucose increased the lag time of thermophilic spores and induced germination of psychrotolerants, whereas L-alanine and L-valine increased the germination efficiency, except alanine for A43. FTIR spectra showed important modifications to the structural components of spores after Fe irradiation at 250 Gy, which could explain the block in spore germination, whereas minor changes were observed after He radiation that could be related to the increased permeability of the inner membranes and alterations of receptor complex structures. Our results give new insights on HZE resistance of extremophiles that are useful in different contexts, including astrobiology. MDPI 2020-10-30 /pmc/articles/PMC7693761/ /pubmed/33143156 http://dx.doi.org/10.3390/life10110264 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zammuto, Vincenzo
Rizzo, Maria G.
De Plano, Laura M.
Franco, Domenico
Guglielmino, Salvatore
Caccamo, Maria T.
Magazù, Salvatore
Fujimori, Akira
Giudice, Angelina Lo
Guglielmin, Mauro
McAlpin, Kevin Roderick
Moeller, Ralf
Gugliandolo, Concetta
Effects of Heavy Ion Particle Irradiation on Spore Germination of Bacillus spp. from Extremely Hot and Cold Environments
title Effects of Heavy Ion Particle Irradiation on Spore Germination of Bacillus spp. from Extremely Hot and Cold Environments
title_full Effects of Heavy Ion Particle Irradiation on Spore Germination of Bacillus spp. from Extremely Hot and Cold Environments
title_fullStr Effects of Heavy Ion Particle Irradiation on Spore Germination of Bacillus spp. from Extremely Hot and Cold Environments
title_full_unstemmed Effects of Heavy Ion Particle Irradiation on Spore Germination of Bacillus spp. from Extremely Hot and Cold Environments
title_short Effects of Heavy Ion Particle Irradiation on Spore Germination of Bacillus spp. from Extremely Hot and Cold Environments
title_sort effects of heavy ion particle irradiation on spore germination of bacillus spp. from extremely hot and cold environments
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7693761/
https://www.ncbi.nlm.nih.gov/pubmed/33143156
http://dx.doi.org/10.3390/life10110264
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