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Effectiveness of Chemical Compounds Used against African Swine Fever Virus in Commercial Available Disinfectants

African swine fever (ASF) causes huge economic losses and is one of most dangerous diseases of pigs. The disease is known for almost 100 years, an effective vaccine or treatment is still unavailable, only proper biosecurity measures, including disinfection, are being applied, in order to prevent dis...

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Autores principales: Juszkiewicz, Małgorzata, Walczak, Marek, Mazur-Panasiuk, Natalia, Woźniakowski, Grzegorz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7693804/
https://www.ncbi.nlm.nih.gov/pubmed/33114391
http://dx.doi.org/10.3390/pathogens9110878
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author Juszkiewicz, Małgorzata
Walczak, Marek
Mazur-Panasiuk, Natalia
Woźniakowski, Grzegorz
author_facet Juszkiewicz, Małgorzata
Walczak, Marek
Mazur-Panasiuk, Natalia
Woźniakowski, Grzegorz
author_sort Juszkiewicz, Małgorzata
collection PubMed
description African swine fever (ASF) causes huge economic losses and is one of most dangerous diseases of pigs. The disease is known for almost 100 years, an effective vaccine or treatment is still unavailable, only proper biosecurity measures, including disinfection, are being applied, in order to prevent disease outbreaks. Eight active substances, i.e., formaldehyde, sodium hypochlorite, caustic soda, glutaraldehyde, phenol, benzalkonium chloride, potassium peroxymonosulfate and acetic acid, were tested, in order to confirm their effectiveness against African swine fever virus (ASFV). This specific selection was done based on the World Organisation for Animal Health (OIE)’s recommendation and previous disinfectant studies on surfaces. The result of our study shows that most of them inactivate the virus, in recommended concentrations. In order to reduce the cytotoxicity of the four substances, Microspin S-400 HR columns were applied, therefore making it possible to demonstrate four logarithms virus titer reduction. Sodium hypochlorite, glutaraldehyde, caustic soda and potassium peroxymonosulfate showed the best ASFV inactivation rates, achieving titer reductions over 5 logs. Despite microfiltration, the virucidal activity of formaldehyde was not assessable, due to its high cytotoxicity. Our results showed that cleaning is particularly important, because removal of the soiling provides improved effectiveness of the tested chemical compounds.
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spelling pubmed-76938042020-11-28 Effectiveness of Chemical Compounds Used against African Swine Fever Virus in Commercial Available Disinfectants Juszkiewicz, Małgorzata Walczak, Marek Mazur-Panasiuk, Natalia Woźniakowski, Grzegorz Pathogens Article African swine fever (ASF) causes huge economic losses and is one of most dangerous diseases of pigs. The disease is known for almost 100 years, an effective vaccine or treatment is still unavailable, only proper biosecurity measures, including disinfection, are being applied, in order to prevent disease outbreaks. Eight active substances, i.e., formaldehyde, sodium hypochlorite, caustic soda, glutaraldehyde, phenol, benzalkonium chloride, potassium peroxymonosulfate and acetic acid, were tested, in order to confirm their effectiveness against African swine fever virus (ASFV). This specific selection was done based on the World Organisation for Animal Health (OIE)’s recommendation and previous disinfectant studies on surfaces. The result of our study shows that most of them inactivate the virus, in recommended concentrations. In order to reduce the cytotoxicity of the four substances, Microspin S-400 HR columns were applied, therefore making it possible to demonstrate four logarithms virus titer reduction. Sodium hypochlorite, glutaraldehyde, caustic soda and potassium peroxymonosulfate showed the best ASFV inactivation rates, achieving titer reductions over 5 logs. Despite microfiltration, the virucidal activity of formaldehyde was not assessable, due to its high cytotoxicity. Our results showed that cleaning is particularly important, because removal of the soiling provides improved effectiveness of the tested chemical compounds. MDPI 2020-10-24 /pmc/articles/PMC7693804/ /pubmed/33114391 http://dx.doi.org/10.3390/pathogens9110878 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Juszkiewicz, Małgorzata
Walczak, Marek
Mazur-Panasiuk, Natalia
Woźniakowski, Grzegorz
Effectiveness of Chemical Compounds Used against African Swine Fever Virus in Commercial Available Disinfectants
title Effectiveness of Chemical Compounds Used against African Swine Fever Virus in Commercial Available Disinfectants
title_full Effectiveness of Chemical Compounds Used against African Swine Fever Virus in Commercial Available Disinfectants
title_fullStr Effectiveness of Chemical Compounds Used against African Swine Fever Virus in Commercial Available Disinfectants
title_full_unstemmed Effectiveness of Chemical Compounds Used against African Swine Fever Virus in Commercial Available Disinfectants
title_short Effectiveness of Chemical Compounds Used against African Swine Fever Virus in Commercial Available Disinfectants
title_sort effectiveness of chemical compounds used against african swine fever virus in commercial available disinfectants
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7693804/
https://www.ncbi.nlm.nih.gov/pubmed/33114391
http://dx.doi.org/10.3390/pathogens9110878
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