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A Novel Microfluidic Method Utilizing a Hydrofoil Structure to Improve Circulating Tumor Cell Enrichment: Design and Analytical Validation

Being one of the major pillars of liquid biopsy, isolation and characterization of circulating tumor cells (CTCs) during cancer management provides critical information on the evolution of cancer and has great potential to increase the success of therapies. In this article, we define a novel strateg...

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Autores principales: Özkayar, Gürhan, Mutlu, Ege, Şahin, Şebnem, Demircan Yalçın, Yağmur, Töral, Taylan, Külah, Haluk, Yildirim, Ender, Zorlu, Özge, Özgür, Ebru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7693848/
https://www.ncbi.nlm.nih.gov/pubmed/33143378
http://dx.doi.org/10.3390/mi11110981
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author Özkayar, Gürhan
Mutlu, Ege
Şahin, Şebnem
Demircan Yalçın, Yağmur
Töral, Taylan
Külah, Haluk
Yildirim, Ender
Zorlu, Özge
Özgür, Ebru
author_facet Özkayar, Gürhan
Mutlu, Ege
Şahin, Şebnem
Demircan Yalçın, Yağmur
Töral, Taylan
Külah, Haluk
Yildirim, Ender
Zorlu, Özge
Özgür, Ebru
author_sort Özkayar, Gürhan
collection PubMed
description Being one of the major pillars of liquid biopsy, isolation and characterization of circulating tumor cells (CTCs) during cancer management provides critical information on the evolution of cancer and has great potential to increase the success of therapies. In this article, we define a novel strategy to effectively enrich CTCs from whole blood based on size, utilizing a spiral microfluidic channel embedded with a hydrofoil structure at the downstream of the spiral channel. The hydrofoil increases the distance between the streams of CTCs and peripheral blood cells, which are already distributed about two focal axes by the spiral channel, thereby improving the resolution of the separation. Analytical validation of the system has been carried out using Michigan Cancer Foundation-7 (MCF7) breast cancer cell lines spiked into blood samples from healthy donors, and the performance of the system in terms of white blood cell (WBC) depletion, CTC recovery rate and cell viability has been shown in single or two-step process: by passing the sample once or twice through the microfluidic chip. Single step process yielded high recovery (77.1%), viable (84.7%) CTCs. When the collected cell suspension is re-processed by the same chip, recovery decreases to 65.5%, while the WBC depletion increases to 88.3%, improving the purity. Cell viability of >80% was preserved after two-step process. The novel microfluidic chip is a good candidate for CTC isolation applications requiring high recovery rate and viability, including functional downstream analyses for variety of cancer types.
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spelling pubmed-76938482020-11-28 A Novel Microfluidic Method Utilizing a Hydrofoil Structure to Improve Circulating Tumor Cell Enrichment: Design and Analytical Validation Özkayar, Gürhan Mutlu, Ege Şahin, Şebnem Demircan Yalçın, Yağmur Töral, Taylan Külah, Haluk Yildirim, Ender Zorlu, Özge Özgür, Ebru Micromachines (Basel) Article Being one of the major pillars of liquid biopsy, isolation and characterization of circulating tumor cells (CTCs) during cancer management provides critical information on the evolution of cancer and has great potential to increase the success of therapies. In this article, we define a novel strategy to effectively enrich CTCs from whole blood based on size, utilizing a spiral microfluidic channel embedded with a hydrofoil structure at the downstream of the spiral channel. The hydrofoil increases the distance between the streams of CTCs and peripheral blood cells, which are already distributed about two focal axes by the spiral channel, thereby improving the resolution of the separation. Analytical validation of the system has been carried out using Michigan Cancer Foundation-7 (MCF7) breast cancer cell lines spiked into blood samples from healthy donors, and the performance of the system in terms of white blood cell (WBC) depletion, CTC recovery rate and cell viability has been shown in single or two-step process: by passing the sample once or twice through the microfluidic chip. Single step process yielded high recovery (77.1%), viable (84.7%) CTCs. When the collected cell suspension is re-processed by the same chip, recovery decreases to 65.5%, while the WBC depletion increases to 88.3%, improving the purity. Cell viability of >80% was preserved after two-step process. The novel microfluidic chip is a good candidate for CTC isolation applications requiring high recovery rate and viability, including functional downstream analyses for variety of cancer types. MDPI 2020-10-30 /pmc/articles/PMC7693848/ /pubmed/33143378 http://dx.doi.org/10.3390/mi11110981 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Özkayar, Gürhan
Mutlu, Ege
Şahin, Şebnem
Demircan Yalçın, Yağmur
Töral, Taylan
Külah, Haluk
Yildirim, Ender
Zorlu, Özge
Özgür, Ebru
A Novel Microfluidic Method Utilizing a Hydrofoil Structure to Improve Circulating Tumor Cell Enrichment: Design and Analytical Validation
title A Novel Microfluidic Method Utilizing a Hydrofoil Structure to Improve Circulating Tumor Cell Enrichment: Design and Analytical Validation
title_full A Novel Microfluidic Method Utilizing a Hydrofoil Structure to Improve Circulating Tumor Cell Enrichment: Design and Analytical Validation
title_fullStr A Novel Microfluidic Method Utilizing a Hydrofoil Structure to Improve Circulating Tumor Cell Enrichment: Design and Analytical Validation
title_full_unstemmed A Novel Microfluidic Method Utilizing a Hydrofoil Structure to Improve Circulating Tumor Cell Enrichment: Design and Analytical Validation
title_short A Novel Microfluidic Method Utilizing a Hydrofoil Structure to Improve Circulating Tumor Cell Enrichment: Design and Analytical Validation
title_sort novel microfluidic method utilizing a hydrofoil structure to improve circulating tumor cell enrichment: design and analytical validation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7693848/
https://www.ncbi.nlm.nih.gov/pubmed/33143378
http://dx.doi.org/10.3390/mi11110981
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