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Exposure to TiO(2) Nanoparticles Increases Listeria monocytogenes Infection of Intestinal Epithelial Cells
Titanium dioxide nanoparticles (TiO(2) NPs) are widely used in a variety of consumer products. Cellular exposure to TiO(2) NPs results in complex effects on cell physiology that could impact biological systems. We investigated the behavior of Listeria monocytogenes in intestinal epithelial cells pre...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7693858/ https://www.ncbi.nlm.nih.gov/pubmed/33158026 http://dx.doi.org/10.3390/nano10112196 |
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author | Ammendolia, Maria Grazia De Berardis, Barbara Maurizi, Linda Longhi, Catia |
author_facet | Ammendolia, Maria Grazia De Berardis, Barbara Maurizi, Linda Longhi, Catia |
author_sort | Ammendolia, Maria Grazia |
collection | PubMed |
description | Titanium dioxide nanoparticles (TiO(2) NPs) are widely used in a variety of consumer products. Cellular exposure to TiO(2) NPs results in complex effects on cell physiology that could impact biological systems. We investigated the behavior of Listeria monocytogenes in intestinal epithelial cells pre-treated with either a low or high (1 and 20 µg/cm(2)) dose of TiO(2) NPs. Our results indicate that the pre-treated cells with a low dose became more permissive to listeria infection; indeed, both adhesion and invasion were significantly increased compared to control. Increased invasion seems to be correlated to cytoskeletal alterations induced by nanoparticles, and higher bacterial survival might be due to the high levels of listeriolysin O that protects L. monocytogenes from reactive oxygen species (ROS). The potential risk of increased susceptibility to L. monocytogenes infection related to long-term intake of nanosized TiO(2) at low doses should be considered. |
format | Online Article Text |
id | pubmed-7693858 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-76938582020-11-28 Exposure to TiO(2) Nanoparticles Increases Listeria monocytogenes Infection of Intestinal Epithelial Cells Ammendolia, Maria Grazia De Berardis, Barbara Maurizi, Linda Longhi, Catia Nanomaterials (Basel) Article Titanium dioxide nanoparticles (TiO(2) NPs) are widely used in a variety of consumer products. Cellular exposure to TiO(2) NPs results in complex effects on cell physiology that could impact biological systems. We investigated the behavior of Listeria monocytogenes in intestinal epithelial cells pre-treated with either a low or high (1 and 20 µg/cm(2)) dose of TiO(2) NPs. Our results indicate that the pre-treated cells with a low dose became more permissive to listeria infection; indeed, both adhesion and invasion were significantly increased compared to control. Increased invasion seems to be correlated to cytoskeletal alterations induced by nanoparticles, and higher bacterial survival might be due to the high levels of listeriolysin O that protects L. monocytogenes from reactive oxygen species (ROS). The potential risk of increased susceptibility to L. monocytogenes infection related to long-term intake of nanosized TiO(2) at low doses should be considered. MDPI 2020-11-04 /pmc/articles/PMC7693858/ /pubmed/33158026 http://dx.doi.org/10.3390/nano10112196 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ammendolia, Maria Grazia De Berardis, Barbara Maurizi, Linda Longhi, Catia Exposure to TiO(2) Nanoparticles Increases Listeria monocytogenes Infection of Intestinal Epithelial Cells |
title | Exposure to TiO(2) Nanoparticles Increases Listeria monocytogenes Infection of Intestinal Epithelial Cells |
title_full | Exposure to TiO(2) Nanoparticles Increases Listeria monocytogenes Infection of Intestinal Epithelial Cells |
title_fullStr | Exposure to TiO(2) Nanoparticles Increases Listeria monocytogenes Infection of Intestinal Epithelial Cells |
title_full_unstemmed | Exposure to TiO(2) Nanoparticles Increases Listeria monocytogenes Infection of Intestinal Epithelial Cells |
title_short | Exposure to TiO(2) Nanoparticles Increases Listeria monocytogenes Infection of Intestinal Epithelial Cells |
title_sort | exposure to tio(2) nanoparticles increases listeria monocytogenes infection of intestinal epithelial cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7693858/ https://www.ncbi.nlm.nih.gov/pubmed/33158026 http://dx.doi.org/10.3390/nano10112196 |
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