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Evaluation of Cytotoxicity and Taste-Masking Effect of Selected Flavors on Dental Lidocaine HCl Injection

Aim: Anxiety and intolerance to dental local anesthetic injections are common in patients undergoing dental procedures. This work was designed to study cytotoxicity of selected flavors in primary gingival keratinocytes (PGK), to acquire information on their suitability for use in dental lidocaine hy...

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Autores principales: Boddu, Sai H. S., Tukaramrao, Diwakar B., Al-Tabakha, Moawia M., Ashames, Akram, Neupane, Rabin, Babu, R. Jayachandra, Renukuntla, Jwala, Tiwari, Amit K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7693877/
https://www.ncbi.nlm.nih.gov/pubmed/33138130
http://dx.doi.org/10.3390/ph13110353
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author Boddu, Sai H. S.
Tukaramrao, Diwakar B.
Al-Tabakha, Moawia M.
Ashames, Akram
Neupane, Rabin
Babu, R. Jayachandra
Renukuntla, Jwala
Tiwari, Amit K.
author_facet Boddu, Sai H. S.
Tukaramrao, Diwakar B.
Al-Tabakha, Moawia M.
Ashames, Akram
Neupane, Rabin
Babu, R. Jayachandra
Renukuntla, Jwala
Tiwari, Amit K.
author_sort Boddu, Sai H. S.
collection PubMed
description Aim: Anxiety and intolerance to dental local anesthetic injections are common in patients undergoing dental procedures. This work was designed to study cytotoxicity of selected flavors in primary gingival keratinocytes (PGK), to acquire information on their suitability for use in dental lidocaine hydrochloride (LID) injection. We also evaluated the bio-mimetic taste of LID dental injection in the presence of selected flavors and sweetener using an Astree electronic tongue (ETongue). Methods: The cytotoxicity of chocolate natural and artificial flavor (CTE), raspberry flavor artificial (RAS), cherry flavor (CHR), bitterness suppressor flavor (BSF) and lemon flavor extract (LFE) at various dilutions (0.16–10% v/v) was carried out in PGK using the live cell morphological analysis and MTT cell cytotoxicity assay. Based on the cytotoxicity data, CTE and RAS were added to Xylocaine(®) (2%) along with 0.09% sodium saccharin and taste was assessed using an ETongue. Results: After three hours of treatment, a dose-dependent cell death was induced by all flavors compared to the untreated control. BSF was found to be more toxic when compared to other flavors. CTE was found to be less toxic. The mean IC50 values of CTE, RAS, CHR, BSF and LFE in PGK were found to be 9.54, 8.43, 2.21, 0.38 and 4.01 mg/mL. Taste analysis with the ETongue showed a clear taste difference between the control and test formulations containing CTE and RAS flavors along with sodium saccharin. Conclusion: CTE and RAS flavors in combination with 0.09% sodium saccharin can achieve a significant taste-masking effect in the dental LID injection.
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spelling pubmed-76938772020-11-28 Evaluation of Cytotoxicity and Taste-Masking Effect of Selected Flavors on Dental Lidocaine HCl Injection Boddu, Sai H. S. Tukaramrao, Diwakar B. Al-Tabakha, Moawia M. Ashames, Akram Neupane, Rabin Babu, R. Jayachandra Renukuntla, Jwala Tiwari, Amit K. Pharmaceuticals (Basel) Article Aim: Anxiety and intolerance to dental local anesthetic injections are common in patients undergoing dental procedures. This work was designed to study cytotoxicity of selected flavors in primary gingival keratinocytes (PGK), to acquire information on their suitability for use in dental lidocaine hydrochloride (LID) injection. We also evaluated the bio-mimetic taste of LID dental injection in the presence of selected flavors and sweetener using an Astree electronic tongue (ETongue). Methods: The cytotoxicity of chocolate natural and artificial flavor (CTE), raspberry flavor artificial (RAS), cherry flavor (CHR), bitterness suppressor flavor (BSF) and lemon flavor extract (LFE) at various dilutions (0.16–10% v/v) was carried out in PGK using the live cell morphological analysis and MTT cell cytotoxicity assay. Based on the cytotoxicity data, CTE and RAS were added to Xylocaine(®) (2%) along with 0.09% sodium saccharin and taste was assessed using an ETongue. Results: After three hours of treatment, a dose-dependent cell death was induced by all flavors compared to the untreated control. BSF was found to be more toxic when compared to other flavors. CTE was found to be less toxic. The mean IC50 values of CTE, RAS, CHR, BSF and LFE in PGK were found to be 9.54, 8.43, 2.21, 0.38 and 4.01 mg/mL. Taste analysis with the ETongue showed a clear taste difference between the control and test formulations containing CTE and RAS flavors along with sodium saccharin. Conclusion: CTE and RAS flavors in combination with 0.09% sodium saccharin can achieve a significant taste-masking effect in the dental LID injection. MDPI 2020-10-29 /pmc/articles/PMC7693877/ /pubmed/33138130 http://dx.doi.org/10.3390/ph13110353 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Boddu, Sai H. S.
Tukaramrao, Diwakar B.
Al-Tabakha, Moawia M.
Ashames, Akram
Neupane, Rabin
Babu, R. Jayachandra
Renukuntla, Jwala
Tiwari, Amit K.
Evaluation of Cytotoxicity and Taste-Masking Effect of Selected Flavors on Dental Lidocaine HCl Injection
title Evaluation of Cytotoxicity and Taste-Masking Effect of Selected Flavors on Dental Lidocaine HCl Injection
title_full Evaluation of Cytotoxicity and Taste-Masking Effect of Selected Flavors on Dental Lidocaine HCl Injection
title_fullStr Evaluation of Cytotoxicity and Taste-Masking Effect of Selected Flavors on Dental Lidocaine HCl Injection
title_full_unstemmed Evaluation of Cytotoxicity and Taste-Masking Effect of Selected Flavors on Dental Lidocaine HCl Injection
title_short Evaluation of Cytotoxicity and Taste-Masking Effect of Selected Flavors on Dental Lidocaine HCl Injection
title_sort evaluation of cytotoxicity and taste-masking effect of selected flavors on dental lidocaine hcl injection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7693877/
https://www.ncbi.nlm.nih.gov/pubmed/33138130
http://dx.doi.org/10.3390/ph13110353
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