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Comparative Genome Analysis of 33 Chlamydia Strains Reveals Characteristic Features of Chlamydia Psittaci and Closely Related Species

To identify genome-based features characteristic of the avian and human pathogen Chlamydia (C.) psittaci and related chlamydiae, we analyzed whole-genome sequences of 33 strains belonging to 12 species. Using a novel genome analysis tool termed Roary ILP Bacterial Annotation Pipeline (RIBAP), this p...

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Autores principales: Hölzer, Martin, Barf, Lisa-Marie, Lamkiewicz, Kevin, Vorimore, Fabien, Lataretu, Marie, Favaroni, Alison, Schnee, Christiane, Laroucau, Karine, Marz, Manja, Sachse, Konrad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7694038/
https://www.ncbi.nlm.nih.gov/pubmed/33126635
http://dx.doi.org/10.3390/pathogens9110899
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author Hölzer, Martin
Barf, Lisa-Marie
Lamkiewicz, Kevin
Vorimore, Fabien
Lataretu, Marie
Favaroni, Alison
Schnee, Christiane
Laroucau, Karine
Marz, Manja
Sachse, Konrad
author_facet Hölzer, Martin
Barf, Lisa-Marie
Lamkiewicz, Kevin
Vorimore, Fabien
Lataretu, Marie
Favaroni, Alison
Schnee, Christiane
Laroucau, Karine
Marz, Manja
Sachse, Konrad
author_sort Hölzer, Martin
collection PubMed
description To identify genome-based features characteristic of the avian and human pathogen Chlamydia (C.) psittaci and related chlamydiae, we analyzed whole-genome sequences of 33 strains belonging to 12 species. Using a novel genome analysis tool termed Roary ILP Bacterial Annotation Pipeline (RIBAP), this panel of strains was shown to share a large core genome comprising 784 genes and representing approximately 80% of individual genomes. Analyzing the most variable genomic sites, we identified a set of features of C. psittaci that in its entirety is characteristic of this species: (i) a relatively short plasticity zone of less than 30,000 nt without a tryptophan operon (also in C. abortus, C. avium, C. gallinacea, C. pneumoniae), (ii) a characteristic set of of Inc proteins comprising IncA, B, C, V, X, Y (with homologs in C. abortus, C. caviae and C. felis as closest relatives), (iii) a 502-aa SinC protein, the largest among Chlamydia spp., and (iv) an elevated number of Pmp proteins of subtype G (14 in C. psittaci, 14 in Cand. C. ibidis). In combination with future functional studies, the common and distinctive criteria revealed in this study provide important clues for understanding the complexity of host-specific behavior of individual Chlamydia spp.
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spelling pubmed-76940382020-11-28 Comparative Genome Analysis of 33 Chlamydia Strains Reveals Characteristic Features of Chlamydia Psittaci and Closely Related Species Hölzer, Martin Barf, Lisa-Marie Lamkiewicz, Kevin Vorimore, Fabien Lataretu, Marie Favaroni, Alison Schnee, Christiane Laroucau, Karine Marz, Manja Sachse, Konrad Pathogens Article To identify genome-based features characteristic of the avian and human pathogen Chlamydia (C.) psittaci and related chlamydiae, we analyzed whole-genome sequences of 33 strains belonging to 12 species. Using a novel genome analysis tool termed Roary ILP Bacterial Annotation Pipeline (RIBAP), this panel of strains was shown to share a large core genome comprising 784 genes and representing approximately 80% of individual genomes. Analyzing the most variable genomic sites, we identified a set of features of C. psittaci that in its entirety is characteristic of this species: (i) a relatively short plasticity zone of less than 30,000 nt without a tryptophan operon (also in C. abortus, C. avium, C. gallinacea, C. pneumoniae), (ii) a characteristic set of of Inc proteins comprising IncA, B, C, V, X, Y (with homologs in C. abortus, C. caviae and C. felis as closest relatives), (iii) a 502-aa SinC protein, the largest among Chlamydia spp., and (iv) an elevated number of Pmp proteins of subtype G (14 in C. psittaci, 14 in Cand. C. ibidis). In combination with future functional studies, the common and distinctive criteria revealed in this study provide important clues for understanding the complexity of host-specific behavior of individual Chlamydia spp. MDPI 2020-10-28 /pmc/articles/PMC7694038/ /pubmed/33126635 http://dx.doi.org/10.3390/pathogens9110899 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hölzer, Martin
Barf, Lisa-Marie
Lamkiewicz, Kevin
Vorimore, Fabien
Lataretu, Marie
Favaroni, Alison
Schnee, Christiane
Laroucau, Karine
Marz, Manja
Sachse, Konrad
Comparative Genome Analysis of 33 Chlamydia Strains Reveals Characteristic Features of Chlamydia Psittaci and Closely Related Species
title Comparative Genome Analysis of 33 Chlamydia Strains Reveals Characteristic Features of Chlamydia Psittaci and Closely Related Species
title_full Comparative Genome Analysis of 33 Chlamydia Strains Reveals Characteristic Features of Chlamydia Psittaci and Closely Related Species
title_fullStr Comparative Genome Analysis of 33 Chlamydia Strains Reveals Characteristic Features of Chlamydia Psittaci and Closely Related Species
title_full_unstemmed Comparative Genome Analysis of 33 Chlamydia Strains Reveals Characteristic Features of Chlamydia Psittaci and Closely Related Species
title_short Comparative Genome Analysis of 33 Chlamydia Strains Reveals Characteristic Features of Chlamydia Psittaci and Closely Related Species
title_sort comparative genome analysis of 33 chlamydia strains reveals characteristic features of chlamydia psittaci and closely related species
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7694038/
https://www.ncbi.nlm.nih.gov/pubmed/33126635
http://dx.doi.org/10.3390/pathogens9110899
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