Cargando…
Marine-Derived Penicillium purpurogenum Reduces Tumor Size and Ameliorates Inflammation in an Erlich Mice Model
Background: This study addresses the antitumoral properties of Penicillium purpurogenum isolated from a polluted lagoon in Northeastern Brazil. Methods: Ethyl Acetate Extracellular Extract (EAE) was used. The metabolites were studied using direct infusion mass spectrometry. The solid Ehrlich tumor m...
Autores principales: | , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7694122/ https://www.ncbi.nlm.nih.gov/pubmed/33138062 http://dx.doi.org/10.3390/md18110541 |
_version_ | 1783614904143446016 |
---|---|
author | Teles, Amanda Mara Pontes, Leticia Prince Pereira Guimarães, Sulayne Janayna Araújo Butarelli, Ana Luiza Silva, Gabriel Xavier do Nascimento, Flavia Raquel Fernandes de Barros Bezerra, Geusa Felipa Moragas-Tellis, Carla Junqueira da Costa, Rui Miguel Gil da Silva, Marcos Antonio Custódio Neto Almeida-Souza, Fernando da Silva Calabrese, Kátia Azevedo-Santos, Ana Paula Silva Nascimento, Maria do Desterro Soares Brandão |
author_facet | Teles, Amanda Mara Pontes, Leticia Prince Pereira Guimarães, Sulayne Janayna Araújo Butarelli, Ana Luiza Silva, Gabriel Xavier do Nascimento, Flavia Raquel Fernandes de Barros Bezerra, Geusa Felipa Moragas-Tellis, Carla Junqueira da Costa, Rui Miguel Gil da Silva, Marcos Antonio Custódio Neto Almeida-Souza, Fernando da Silva Calabrese, Kátia Azevedo-Santos, Ana Paula Silva Nascimento, Maria do Desterro Soares Brandão |
author_sort | Teles, Amanda Mara |
collection | PubMed |
description | Background: This study addresses the antitumoral properties of Penicillium purpurogenum isolated from a polluted lagoon in Northeastern Brazil. Methods: Ethyl Acetate Extracellular Extract (EAE) was used. The metabolites were studied using direct infusion mass spectrometry. The solid Ehrlich tumor model was used for antitumor activity. Female Swiss mice were divided into groups (n = 10/group) as follows: The negative control (CTL−), treated with a phosphate buffered solution; the positive control (CTL+), treated with cyclophosphamide (25 mg/kg); extract treatments at doses of 4, 20, and 100 mg/kg; animals without tumors or treatments (Sham); and animals without tumors treated with an intermediate dose (EAE20). All treatments were performed intraperitoneally, daily, for 15 days. Subsequently, the animals were euthanized, and the tumor, lymphoid organs, and serum were used for immunological, histological, and biochemical parameter evaluations. Results: The extract was rich in meroterpenoids. All doses significantly reduced tumor size, and the 20 and 100 mg/kg doses reduced tumor-associated inflammation and tumor necrosis. The extract also reduced the cellular infiltration of lymphoid organs and circulating TNF-α levels. The extract did not induce weight loss or renal and hepatic toxic changes. Conclusions: These results indicate that P. purpurogenum exhibits immunomodulatory and antitumor properties in vivo. Thus, fungal fermentation is a valid biotechnological approach to the production of antitumor agents. |
format | Online Article Text |
id | pubmed-7694122 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-76941222020-11-28 Marine-Derived Penicillium purpurogenum Reduces Tumor Size and Ameliorates Inflammation in an Erlich Mice Model Teles, Amanda Mara Pontes, Leticia Prince Pereira Guimarães, Sulayne Janayna Araújo Butarelli, Ana Luiza Silva, Gabriel Xavier do Nascimento, Flavia Raquel Fernandes de Barros Bezerra, Geusa Felipa Moragas-Tellis, Carla Junqueira da Costa, Rui Miguel Gil da Silva, Marcos Antonio Custódio Neto Almeida-Souza, Fernando da Silva Calabrese, Kátia Azevedo-Santos, Ana Paula Silva Nascimento, Maria do Desterro Soares Brandão Mar Drugs Article Background: This study addresses the antitumoral properties of Penicillium purpurogenum isolated from a polluted lagoon in Northeastern Brazil. Methods: Ethyl Acetate Extracellular Extract (EAE) was used. The metabolites were studied using direct infusion mass spectrometry. The solid Ehrlich tumor model was used for antitumor activity. Female Swiss mice were divided into groups (n = 10/group) as follows: The negative control (CTL−), treated with a phosphate buffered solution; the positive control (CTL+), treated with cyclophosphamide (25 mg/kg); extract treatments at doses of 4, 20, and 100 mg/kg; animals without tumors or treatments (Sham); and animals without tumors treated with an intermediate dose (EAE20). All treatments were performed intraperitoneally, daily, for 15 days. Subsequently, the animals were euthanized, and the tumor, lymphoid organs, and serum were used for immunological, histological, and biochemical parameter evaluations. Results: The extract was rich in meroterpenoids. All doses significantly reduced tumor size, and the 20 and 100 mg/kg doses reduced tumor-associated inflammation and tumor necrosis. The extract also reduced the cellular infiltration of lymphoid organs and circulating TNF-α levels. The extract did not induce weight loss or renal and hepatic toxic changes. Conclusions: These results indicate that P. purpurogenum exhibits immunomodulatory and antitumor properties in vivo. Thus, fungal fermentation is a valid biotechnological approach to the production of antitumor agents. MDPI 2020-10-29 /pmc/articles/PMC7694122/ /pubmed/33138062 http://dx.doi.org/10.3390/md18110541 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Teles, Amanda Mara Pontes, Leticia Prince Pereira Guimarães, Sulayne Janayna Araújo Butarelli, Ana Luiza Silva, Gabriel Xavier do Nascimento, Flavia Raquel Fernandes de Barros Bezerra, Geusa Felipa Moragas-Tellis, Carla Junqueira da Costa, Rui Miguel Gil da Silva, Marcos Antonio Custódio Neto Almeida-Souza, Fernando da Silva Calabrese, Kátia Azevedo-Santos, Ana Paula Silva Nascimento, Maria do Desterro Soares Brandão Marine-Derived Penicillium purpurogenum Reduces Tumor Size and Ameliorates Inflammation in an Erlich Mice Model |
title | Marine-Derived Penicillium purpurogenum Reduces Tumor Size and Ameliorates Inflammation in an Erlich Mice Model |
title_full | Marine-Derived Penicillium purpurogenum Reduces Tumor Size and Ameliorates Inflammation in an Erlich Mice Model |
title_fullStr | Marine-Derived Penicillium purpurogenum Reduces Tumor Size and Ameliorates Inflammation in an Erlich Mice Model |
title_full_unstemmed | Marine-Derived Penicillium purpurogenum Reduces Tumor Size and Ameliorates Inflammation in an Erlich Mice Model |
title_short | Marine-Derived Penicillium purpurogenum Reduces Tumor Size and Ameliorates Inflammation in an Erlich Mice Model |
title_sort | marine-derived penicillium purpurogenum reduces tumor size and ameliorates inflammation in an erlich mice model |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7694122/ https://www.ncbi.nlm.nih.gov/pubmed/33138062 http://dx.doi.org/10.3390/md18110541 |
work_keys_str_mv | AT telesamandamara marinederivedpenicilliumpurpurogenumreducestumorsizeandamelioratesinflammationinanerlichmicemodel AT pontesleticiaprincepereira marinederivedpenicilliumpurpurogenumreducestumorsizeandamelioratesinflammationinanerlichmicemodel AT guimaraessulaynejanaynaaraujo marinederivedpenicilliumpurpurogenumreducestumorsizeandamelioratesinflammationinanerlichmicemodel AT butarellianaluiza marinederivedpenicilliumpurpurogenumreducestumorsizeandamelioratesinflammationinanerlichmicemodel AT silvagabrielxavier marinederivedpenicilliumpurpurogenumreducestumorsizeandamelioratesinflammationinanerlichmicemodel AT donascimentoflaviaraquelfernandes marinederivedpenicilliumpurpurogenumreducestumorsizeandamelioratesinflammationinanerlichmicemodel AT debarrosbezerrageusafelipa marinederivedpenicilliumpurpurogenumreducestumorsizeandamelioratesinflammationinanerlichmicemodel AT moragastelliscarlajunqueira marinederivedpenicilliumpurpurogenumreducestumorsizeandamelioratesinflammationinanerlichmicemodel AT dacostaruimiguelgil marinederivedpenicilliumpurpurogenumreducestumorsizeandamelioratesinflammationinanerlichmicemodel AT dasilvamarcosantoniocustodioneto marinederivedpenicilliumpurpurogenumreducestumorsizeandamelioratesinflammationinanerlichmicemodel AT almeidasouzafernando marinederivedpenicilliumpurpurogenumreducestumorsizeandamelioratesinflammationinanerlichmicemodel AT dasilvacalabresekatia marinederivedpenicilliumpurpurogenumreducestumorsizeandamelioratesinflammationinanerlichmicemodel AT azevedosantosanapaulasilva marinederivedpenicilliumpurpurogenumreducestumorsizeandamelioratesinflammationinanerlichmicemodel AT nascimentomariadodesterrosoaresbrandao marinederivedpenicilliumpurpurogenumreducestumorsizeandamelioratesinflammationinanerlichmicemodel |