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Marine-Derived Penicillium purpurogenum Reduces Tumor Size and Ameliorates Inflammation in an Erlich Mice Model

Background: This study addresses the antitumoral properties of Penicillium purpurogenum isolated from a polluted lagoon in Northeastern Brazil. Methods: Ethyl Acetate Extracellular Extract (EAE) was used. The metabolites were studied using direct infusion mass spectrometry. The solid Ehrlich tumor m...

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Autores principales: Teles, Amanda Mara, Pontes, Leticia Prince Pereira, Guimarães, Sulayne Janayna Araújo, Butarelli, Ana Luiza, Silva, Gabriel Xavier, do Nascimento, Flavia Raquel Fernandes, de Barros Bezerra, Geusa Felipa, Moragas-Tellis, Carla Junqueira, da Costa, Rui Miguel Gil, da Silva, Marcos Antonio Custódio Neto, Almeida-Souza, Fernando, da Silva Calabrese, Kátia, Azevedo-Santos, Ana Paula Silva, Nascimento, Maria do Desterro Soares Brandão
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7694122/
https://www.ncbi.nlm.nih.gov/pubmed/33138062
http://dx.doi.org/10.3390/md18110541
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author Teles, Amanda Mara
Pontes, Leticia Prince Pereira
Guimarães, Sulayne Janayna Araújo
Butarelli, Ana Luiza
Silva, Gabriel Xavier
do Nascimento, Flavia Raquel Fernandes
de Barros Bezerra, Geusa Felipa
Moragas-Tellis, Carla Junqueira
da Costa, Rui Miguel Gil
da Silva, Marcos Antonio Custódio Neto
Almeida-Souza, Fernando
da Silva Calabrese, Kátia
Azevedo-Santos, Ana Paula Silva
Nascimento, Maria do Desterro Soares Brandão
author_facet Teles, Amanda Mara
Pontes, Leticia Prince Pereira
Guimarães, Sulayne Janayna Araújo
Butarelli, Ana Luiza
Silva, Gabriel Xavier
do Nascimento, Flavia Raquel Fernandes
de Barros Bezerra, Geusa Felipa
Moragas-Tellis, Carla Junqueira
da Costa, Rui Miguel Gil
da Silva, Marcos Antonio Custódio Neto
Almeida-Souza, Fernando
da Silva Calabrese, Kátia
Azevedo-Santos, Ana Paula Silva
Nascimento, Maria do Desterro Soares Brandão
author_sort Teles, Amanda Mara
collection PubMed
description Background: This study addresses the antitumoral properties of Penicillium purpurogenum isolated from a polluted lagoon in Northeastern Brazil. Methods: Ethyl Acetate Extracellular Extract (EAE) was used. The metabolites were studied using direct infusion mass spectrometry. The solid Ehrlich tumor model was used for antitumor activity. Female Swiss mice were divided into groups (n = 10/group) as follows: The negative control (CTL−), treated with a phosphate buffered solution; the positive control (CTL+), treated with cyclophosphamide (25 mg/kg); extract treatments at doses of 4, 20, and 100 mg/kg; animals without tumors or treatments (Sham); and animals without tumors treated with an intermediate dose (EAE20). All treatments were performed intraperitoneally, daily, for 15 days. Subsequently, the animals were euthanized, and the tumor, lymphoid organs, and serum were used for immunological, histological, and biochemical parameter evaluations. Results: The extract was rich in meroterpenoids. All doses significantly reduced tumor size, and the 20 and 100 mg/kg doses reduced tumor-associated inflammation and tumor necrosis. The extract also reduced the cellular infiltration of lymphoid organs and circulating TNF-α levels. The extract did not induce weight loss or renal and hepatic toxic changes. Conclusions: These results indicate that P. purpurogenum exhibits immunomodulatory and antitumor properties in vivo. Thus, fungal fermentation is a valid biotechnological approach to the production of antitumor agents.
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spelling pubmed-76941222020-11-28 Marine-Derived Penicillium purpurogenum Reduces Tumor Size and Ameliorates Inflammation in an Erlich Mice Model Teles, Amanda Mara Pontes, Leticia Prince Pereira Guimarães, Sulayne Janayna Araújo Butarelli, Ana Luiza Silva, Gabriel Xavier do Nascimento, Flavia Raquel Fernandes de Barros Bezerra, Geusa Felipa Moragas-Tellis, Carla Junqueira da Costa, Rui Miguel Gil da Silva, Marcos Antonio Custódio Neto Almeida-Souza, Fernando da Silva Calabrese, Kátia Azevedo-Santos, Ana Paula Silva Nascimento, Maria do Desterro Soares Brandão Mar Drugs Article Background: This study addresses the antitumoral properties of Penicillium purpurogenum isolated from a polluted lagoon in Northeastern Brazil. Methods: Ethyl Acetate Extracellular Extract (EAE) was used. The metabolites were studied using direct infusion mass spectrometry. The solid Ehrlich tumor model was used for antitumor activity. Female Swiss mice were divided into groups (n = 10/group) as follows: The negative control (CTL−), treated with a phosphate buffered solution; the positive control (CTL+), treated with cyclophosphamide (25 mg/kg); extract treatments at doses of 4, 20, and 100 mg/kg; animals without tumors or treatments (Sham); and animals without tumors treated with an intermediate dose (EAE20). All treatments were performed intraperitoneally, daily, for 15 days. Subsequently, the animals were euthanized, and the tumor, lymphoid organs, and serum were used for immunological, histological, and biochemical parameter evaluations. Results: The extract was rich in meroterpenoids. All doses significantly reduced tumor size, and the 20 and 100 mg/kg doses reduced tumor-associated inflammation and tumor necrosis. The extract also reduced the cellular infiltration of lymphoid organs and circulating TNF-α levels. The extract did not induce weight loss or renal and hepatic toxic changes. Conclusions: These results indicate that P. purpurogenum exhibits immunomodulatory and antitumor properties in vivo. Thus, fungal fermentation is a valid biotechnological approach to the production of antitumor agents. MDPI 2020-10-29 /pmc/articles/PMC7694122/ /pubmed/33138062 http://dx.doi.org/10.3390/md18110541 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Teles, Amanda Mara
Pontes, Leticia Prince Pereira
Guimarães, Sulayne Janayna Araújo
Butarelli, Ana Luiza
Silva, Gabriel Xavier
do Nascimento, Flavia Raquel Fernandes
de Barros Bezerra, Geusa Felipa
Moragas-Tellis, Carla Junqueira
da Costa, Rui Miguel Gil
da Silva, Marcos Antonio Custódio Neto
Almeida-Souza, Fernando
da Silva Calabrese, Kátia
Azevedo-Santos, Ana Paula Silva
Nascimento, Maria do Desterro Soares Brandão
Marine-Derived Penicillium purpurogenum Reduces Tumor Size and Ameliorates Inflammation in an Erlich Mice Model
title Marine-Derived Penicillium purpurogenum Reduces Tumor Size and Ameliorates Inflammation in an Erlich Mice Model
title_full Marine-Derived Penicillium purpurogenum Reduces Tumor Size and Ameliorates Inflammation in an Erlich Mice Model
title_fullStr Marine-Derived Penicillium purpurogenum Reduces Tumor Size and Ameliorates Inflammation in an Erlich Mice Model
title_full_unstemmed Marine-Derived Penicillium purpurogenum Reduces Tumor Size and Ameliorates Inflammation in an Erlich Mice Model
title_short Marine-Derived Penicillium purpurogenum Reduces Tumor Size and Ameliorates Inflammation in an Erlich Mice Model
title_sort marine-derived penicillium purpurogenum reduces tumor size and ameliorates inflammation in an erlich mice model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7694122/
https://www.ncbi.nlm.nih.gov/pubmed/33138062
http://dx.doi.org/10.3390/md18110541
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