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Self-Assembled Nanocarriers Based on Modified Chitosan for Biomedical Applications: Preparation and Characterization

Protein-polysaccharide systems are of increasing interest as their combined attributes allow for fulfilling a broad range of applications in biomedical and pharmaceutical fields. In this study, the preparation of nanogels based on maleic anhydride chitosan derivatives (MAC) and bovine serum albumin...

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Autores principales: Rusu, Alina Gabriela, Chiriac, Aurica P., Nita, Loredana Elena, Rosca, Irina, Rusu, Daniela, Neamtu, Iordana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7694257/
https://www.ncbi.nlm.nih.gov/pubmed/33158235
http://dx.doi.org/10.3390/polym12112593
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author Rusu, Alina Gabriela
Chiriac, Aurica P.
Nita, Loredana Elena
Rosca, Irina
Rusu, Daniela
Neamtu, Iordana
author_facet Rusu, Alina Gabriela
Chiriac, Aurica P.
Nita, Loredana Elena
Rosca, Irina
Rusu, Daniela
Neamtu, Iordana
author_sort Rusu, Alina Gabriela
collection PubMed
description Protein-polysaccharide systems are of increasing interest as their combined attributes allow for fulfilling a broad range of applications in biomedical and pharmaceutical fields. In this study, the preparation of nanogels based on maleic anhydride chitosan derivatives (MAC) and bovine serum albumin (BSA) was achieved through a self-assembly process performed in aqueous phase. A series of experiments performed by varying the concentrations of MAC and BSA were conducted to find an appropriate mixing ratio for the polymer solutions leading to thermodynamically stable nanogels with the ability to encapsulate active compounds. The influence of temperature on the formation of nanogels was also studied. The consequent conformational changes were monitored using ultraviolet-visible (UV-VIS) spectrophotometry. The spectrophotometric investigations combined with diffraction light scattering (DLS) technique and zeta potential measurement results were correlated to determine the interaction mechanism and assess the self-assembling processes during nanogel formation. It was found that the hydrodynamic diameter (D(h)) of the nanoparticles increased slightly at acidic pH, and the protonation of ionizable amino groups with the pH was confirmed by the zeta potential measurements. MAC/BSA nanogels also exhibited antimicrobial properties after being loaded with amoxicillin (Amox), which is an antibiotic used for the treatment of various infections. The experimental data resulting from this study provide theoretical guidance for the design and development of attractive nanocarriers for a large variety of biomedical applications.
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spelling pubmed-76942572020-11-28 Self-Assembled Nanocarriers Based on Modified Chitosan for Biomedical Applications: Preparation and Characterization Rusu, Alina Gabriela Chiriac, Aurica P. Nita, Loredana Elena Rosca, Irina Rusu, Daniela Neamtu, Iordana Polymers (Basel) Article Protein-polysaccharide systems are of increasing interest as their combined attributes allow for fulfilling a broad range of applications in biomedical and pharmaceutical fields. In this study, the preparation of nanogels based on maleic anhydride chitosan derivatives (MAC) and bovine serum albumin (BSA) was achieved through a self-assembly process performed in aqueous phase. A series of experiments performed by varying the concentrations of MAC and BSA were conducted to find an appropriate mixing ratio for the polymer solutions leading to thermodynamically stable nanogels with the ability to encapsulate active compounds. The influence of temperature on the formation of nanogels was also studied. The consequent conformational changes were monitored using ultraviolet-visible (UV-VIS) spectrophotometry. The spectrophotometric investigations combined with diffraction light scattering (DLS) technique and zeta potential measurement results were correlated to determine the interaction mechanism and assess the self-assembling processes during nanogel formation. It was found that the hydrodynamic diameter (D(h)) of the nanoparticles increased slightly at acidic pH, and the protonation of ionizable amino groups with the pH was confirmed by the zeta potential measurements. MAC/BSA nanogels also exhibited antimicrobial properties after being loaded with amoxicillin (Amox), which is an antibiotic used for the treatment of various infections. The experimental data resulting from this study provide theoretical guidance for the design and development of attractive nanocarriers for a large variety of biomedical applications. MDPI 2020-11-04 /pmc/articles/PMC7694257/ /pubmed/33158235 http://dx.doi.org/10.3390/polym12112593 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Rusu, Alina Gabriela
Chiriac, Aurica P.
Nita, Loredana Elena
Rosca, Irina
Rusu, Daniela
Neamtu, Iordana
Self-Assembled Nanocarriers Based on Modified Chitosan for Biomedical Applications: Preparation and Characterization
title Self-Assembled Nanocarriers Based on Modified Chitosan for Biomedical Applications: Preparation and Characterization
title_full Self-Assembled Nanocarriers Based on Modified Chitosan for Biomedical Applications: Preparation and Characterization
title_fullStr Self-Assembled Nanocarriers Based on Modified Chitosan for Biomedical Applications: Preparation and Characterization
title_full_unstemmed Self-Assembled Nanocarriers Based on Modified Chitosan for Biomedical Applications: Preparation and Characterization
title_short Self-Assembled Nanocarriers Based on Modified Chitosan for Biomedical Applications: Preparation and Characterization
title_sort self-assembled nanocarriers based on modified chitosan for biomedical applications: preparation and characterization
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7694257/
https://www.ncbi.nlm.nih.gov/pubmed/33158235
http://dx.doi.org/10.3390/polym12112593
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