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Is ECLIA Serum Cortisol Concentration Measurement, an Accurate Indicator of Pain Severity in Dogs with Locomotor Pain?

SIMPLE SUMMARY: Serum cortisol level reflects the activity of stress axis, ethological alterations, acute and chronic pain, life quality, or psychogenic stress. Although it is stated that stress can produce a measurable influence on the cortisol level, a certified value of this pain biomarker in dog...

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Autores principales: Markovszky, Adela Katalin, Weber, Corinna, Biksi, Otília, Danes, Mihai, Dumitrescu, Eugenia, Muselin, Florin, Tufarelli, Vincenzo, Puvača, Nikola, Cristina, Romeo Teodor
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7694258/
https://www.ncbi.nlm.nih.gov/pubmed/33158237
http://dx.doi.org/10.3390/ani10112036
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author Markovszky, Adela Katalin
Weber, Corinna
Biksi, Otília
Danes, Mihai
Dumitrescu, Eugenia
Muselin, Florin
Tufarelli, Vincenzo
Puvača, Nikola
Cristina, Romeo Teodor
author_facet Markovszky, Adela Katalin
Weber, Corinna
Biksi, Otília
Danes, Mihai
Dumitrescu, Eugenia
Muselin, Florin
Tufarelli, Vincenzo
Puvača, Nikola
Cristina, Romeo Teodor
author_sort Markovszky, Adela Katalin
collection PubMed
description SIMPLE SUMMARY: Serum cortisol level reflects the activity of stress axis, ethological alterations, acute and chronic pain, life quality, or psychogenic stress. Although it is stated that stress can produce a measurable influence on the cortisol level, a certified value of this pain biomarker in dogs was not generally accepted yet. This interdisciplinary research emerged from the need for information in this field since not many studies were focused mainly on comparative analysis. We consider this field as a hot topic with various possible applications. Our survey is a methodological study within the fields of behavior and veterinary sciences, being relevant for the dog’s pain assessment. Results are having the guarantee of the high standard analysis of serum specimens, and using the updated Cortisol assay, with serum cortisol determined by Electrochemiluminescence immunoassay. We consider that our work could refresh the information in this field. Yet, an area of interest, specific pathology and pain particularities in a dog, being studied more and more in the last decade. What we can say is that serum cortisol limits cannot be adopted as a single and accurate pain marker in dog species, our study confirming these values, as non-conclusive for the assessment of the real pain levels in the dogs. ABSTRACT: The purpose of determining serum cortisol level is to reflect the activity of stress axis, ethological alterations, acute and chronic pain, life quality, or psychogenic stress. Although it is stated that stress can produce a measurable influence on the cortisol level, a certified value of this pain biomarker in dogs was not generally accepted yet. This study aimed to investigate if serum cortisol measured follows allopathic treatments only, or it is associated with physiotherapy, point out pain level in dogs with orthopedic disease, which could reveal the healing progress. The diagnostic identified: hip dysplasia, cranial cruciate ligament rupture, or intervertebral disc disease. Ortolani and Barden tests, together with clinical examination, drawer sign, and tibia compression test, were done in dogs exhibiting postures, and motion alteration, and X-Ray confirmed. A total of 30 dogs were grouped in healthy (n = 10) and pain groups (n = 20), the blood sampling is done at the beginning of the investigation, and after ten days of the study. Dogs were handled in two ways: G1—treated with Nonsteroidal Anti-Inflammatory Drugs (NSAIDs) only and respectively, G2—by therapy and physiotherapy. The analysis was performed on a Roche Cobas Analyzer (Roche, USA), serum cortisol being determined by Electrochemiluminescence immunoassay (ECLIA), and statistics using ANOVA, following Tukey’s Multiple Comparison Test. The results revealed that, out of ten specimens in the Control group, nine were within the normal limits: 5–65 ng × mL(−1) (24.76 ± 19.48678), and one sample under the set limit. In G1, it was observed that the plasmatic P1 values were below the levels of P2, in six situations. In G2, although the status of all subjects improved radically with the removal or evident reduction of pain, confirmed clinically and imagistically, the P2 values in five dogs were higher than the initial P1 values, and in contradiction with the observed clinical reality. Comparing results, the mean difference in G1 was 0.41, and in G2 = 2.54, with an SD for G1 = 13.38, and G2 = 16.66, registering moderate development. Standard deviation illustrated that the values of treated groups were highly spread throughout the interval, and the serum cortisol assay did not generate significant statistical differences between groups in our case. This inferred the doubt whether the used detection method or values registered correctly indicates the pain levels in dog species.
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spelling pubmed-76942582020-11-28 Is ECLIA Serum Cortisol Concentration Measurement, an Accurate Indicator of Pain Severity in Dogs with Locomotor Pain? Markovszky, Adela Katalin Weber, Corinna Biksi, Otília Danes, Mihai Dumitrescu, Eugenia Muselin, Florin Tufarelli, Vincenzo Puvača, Nikola Cristina, Romeo Teodor Animals (Basel) Article SIMPLE SUMMARY: Serum cortisol level reflects the activity of stress axis, ethological alterations, acute and chronic pain, life quality, or psychogenic stress. Although it is stated that stress can produce a measurable influence on the cortisol level, a certified value of this pain biomarker in dogs was not generally accepted yet. This interdisciplinary research emerged from the need for information in this field since not many studies were focused mainly on comparative analysis. We consider this field as a hot topic with various possible applications. Our survey is a methodological study within the fields of behavior and veterinary sciences, being relevant for the dog’s pain assessment. Results are having the guarantee of the high standard analysis of serum specimens, and using the updated Cortisol assay, with serum cortisol determined by Electrochemiluminescence immunoassay. We consider that our work could refresh the information in this field. Yet, an area of interest, specific pathology and pain particularities in a dog, being studied more and more in the last decade. What we can say is that serum cortisol limits cannot be adopted as a single and accurate pain marker in dog species, our study confirming these values, as non-conclusive for the assessment of the real pain levels in the dogs. ABSTRACT: The purpose of determining serum cortisol level is to reflect the activity of stress axis, ethological alterations, acute and chronic pain, life quality, or psychogenic stress. Although it is stated that stress can produce a measurable influence on the cortisol level, a certified value of this pain biomarker in dogs was not generally accepted yet. This study aimed to investigate if serum cortisol measured follows allopathic treatments only, or it is associated with physiotherapy, point out pain level in dogs with orthopedic disease, which could reveal the healing progress. The diagnostic identified: hip dysplasia, cranial cruciate ligament rupture, or intervertebral disc disease. Ortolani and Barden tests, together with clinical examination, drawer sign, and tibia compression test, were done in dogs exhibiting postures, and motion alteration, and X-Ray confirmed. A total of 30 dogs were grouped in healthy (n = 10) and pain groups (n = 20), the blood sampling is done at the beginning of the investigation, and after ten days of the study. Dogs were handled in two ways: G1—treated with Nonsteroidal Anti-Inflammatory Drugs (NSAIDs) only and respectively, G2—by therapy and physiotherapy. The analysis was performed on a Roche Cobas Analyzer (Roche, USA), serum cortisol being determined by Electrochemiluminescence immunoassay (ECLIA), and statistics using ANOVA, following Tukey’s Multiple Comparison Test. The results revealed that, out of ten specimens in the Control group, nine were within the normal limits: 5–65 ng × mL(−1) (24.76 ± 19.48678), and one sample under the set limit. In G1, it was observed that the plasmatic P1 values were below the levels of P2, in six situations. In G2, although the status of all subjects improved radically with the removal or evident reduction of pain, confirmed clinically and imagistically, the P2 values in five dogs were higher than the initial P1 values, and in contradiction with the observed clinical reality. Comparing results, the mean difference in G1 was 0.41, and in G2 = 2.54, with an SD for G1 = 13.38, and G2 = 16.66, registering moderate development. Standard deviation illustrated that the values of treated groups were highly spread throughout the interval, and the serum cortisol assay did not generate significant statistical differences between groups in our case. This inferred the doubt whether the used detection method or values registered correctly indicates the pain levels in dog species. MDPI 2020-11-04 /pmc/articles/PMC7694258/ /pubmed/33158237 http://dx.doi.org/10.3390/ani10112036 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Markovszky, Adela Katalin
Weber, Corinna
Biksi, Otília
Danes, Mihai
Dumitrescu, Eugenia
Muselin, Florin
Tufarelli, Vincenzo
Puvača, Nikola
Cristina, Romeo Teodor
Is ECLIA Serum Cortisol Concentration Measurement, an Accurate Indicator of Pain Severity in Dogs with Locomotor Pain?
title Is ECLIA Serum Cortisol Concentration Measurement, an Accurate Indicator of Pain Severity in Dogs with Locomotor Pain?
title_full Is ECLIA Serum Cortisol Concentration Measurement, an Accurate Indicator of Pain Severity in Dogs with Locomotor Pain?
title_fullStr Is ECLIA Serum Cortisol Concentration Measurement, an Accurate Indicator of Pain Severity in Dogs with Locomotor Pain?
title_full_unstemmed Is ECLIA Serum Cortisol Concentration Measurement, an Accurate Indicator of Pain Severity in Dogs with Locomotor Pain?
title_short Is ECLIA Serum Cortisol Concentration Measurement, an Accurate Indicator of Pain Severity in Dogs with Locomotor Pain?
title_sort is eclia serum cortisol concentration measurement, an accurate indicator of pain severity in dogs with locomotor pain?
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7694258/
https://www.ncbi.nlm.nih.gov/pubmed/33158237
http://dx.doi.org/10.3390/ani10112036
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