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Antiviral Potential of Sea Urchin Aminated Spinochromes against Herpes Simplex Virus Type 1

Herpes simplex virus type 1 (HSV-1) is one of the most prevalent pathogens worldwide requiring the search for new candidates for the creation of antiherpetic drugs. The ability of sea urchin spinochromes—echinochrome A (EchA) and its aminated analogues, echinamines A (EamA) and B (EamB)—to inhibit d...

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Autores principales: Mishchenko, Natalia P., Krylova, Natalia V., Iunikhina, Olga V., Vasileva, Elena A., Likhatskaya, Galina N., Pislyagin, Evgeny A., Tarbeeva, Darya V., Dmitrenok, Pavel S., Fedoreyev, Sergey A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7694471/
https://www.ncbi.nlm.nih.gov/pubmed/33167501
http://dx.doi.org/10.3390/md18110550
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author Mishchenko, Natalia P.
Krylova, Natalia V.
Iunikhina, Olga V.
Vasileva, Elena A.
Likhatskaya, Galina N.
Pislyagin, Evgeny A.
Tarbeeva, Darya V.
Dmitrenok, Pavel S.
Fedoreyev, Sergey A.
author_facet Mishchenko, Natalia P.
Krylova, Natalia V.
Iunikhina, Olga V.
Vasileva, Elena A.
Likhatskaya, Galina N.
Pislyagin, Evgeny A.
Tarbeeva, Darya V.
Dmitrenok, Pavel S.
Fedoreyev, Sergey A.
author_sort Mishchenko, Natalia P.
collection PubMed
description Herpes simplex virus type 1 (HSV-1) is one of the most prevalent pathogens worldwide requiring the search for new candidates for the creation of antiherpetic drugs. The ability of sea urchin spinochromes—echinochrome A (EchA) and its aminated analogues, echinamines A (EamA) and B (EamB)—to inhibit different stages of HSV-1 infection in Vero cells and to reduce the virus-induced production of reactive oxygen species (ROS) was studied. We found that spinochromes exhibited maximum antiviral activity when HSV-1 was pretreated with these compounds, which indicated the direct effect of spinochromes on HSV-1 particles. EamB and EamA both showed the highest virucidal activity by inhibiting the HSV-1 plaque formation, with a selectivity index (SI) of 80.6 and 50.3, respectively, and a reduction in HSV-1 attachment to cells (SI of 8.5 and 5.8, respectively). EamA and EamB considerably suppressed the early induction of ROS due to the virus infection. The ability of the tested compounds to directly bind to the surface glycoprotein, gD, of HSV-1 was established in silico. The dock score of EchA, EamA, and EamB was −4.75, −5.09, and −5.19 kcal/mol, respectively, which correlated with the SI of the virucidal action of these compounds and explained their ability to suppress the attachment and penetration of the virus into the cells.
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spelling pubmed-76944712020-11-28 Antiviral Potential of Sea Urchin Aminated Spinochromes against Herpes Simplex Virus Type 1 Mishchenko, Natalia P. Krylova, Natalia V. Iunikhina, Olga V. Vasileva, Elena A. Likhatskaya, Galina N. Pislyagin, Evgeny A. Tarbeeva, Darya V. Dmitrenok, Pavel S. Fedoreyev, Sergey A. Mar Drugs Article Herpes simplex virus type 1 (HSV-1) is one of the most prevalent pathogens worldwide requiring the search for new candidates for the creation of antiherpetic drugs. The ability of sea urchin spinochromes—echinochrome A (EchA) and its aminated analogues, echinamines A (EamA) and B (EamB)—to inhibit different stages of HSV-1 infection in Vero cells and to reduce the virus-induced production of reactive oxygen species (ROS) was studied. We found that spinochromes exhibited maximum antiviral activity when HSV-1 was pretreated with these compounds, which indicated the direct effect of spinochromes on HSV-1 particles. EamB and EamA both showed the highest virucidal activity by inhibiting the HSV-1 plaque formation, with a selectivity index (SI) of 80.6 and 50.3, respectively, and a reduction in HSV-1 attachment to cells (SI of 8.5 and 5.8, respectively). EamA and EamB considerably suppressed the early induction of ROS due to the virus infection. The ability of the tested compounds to directly bind to the surface glycoprotein, gD, of HSV-1 was established in silico. The dock score of EchA, EamA, and EamB was −4.75, −5.09, and −5.19 kcal/mol, respectively, which correlated with the SI of the virucidal action of these compounds and explained their ability to suppress the attachment and penetration of the virus into the cells. MDPI 2020-11-05 /pmc/articles/PMC7694471/ /pubmed/33167501 http://dx.doi.org/10.3390/md18110550 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mishchenko, Natalia P.
Krylova, Natalia V.
Iunikhina, Olga V.
Vasileva, Elena A.
Likhatskaya, Galina N.
Pislyagin, Evgeny A.
Tarbeeva, Darya V.
Dmitrenok, Pavel S.
Fedoreyev, Sergey A.
Antiviral Potential of Sea Urchin Aminated Spinochromes against Herpes Simplex Virus Type 1
title Antiviral Potential of Sea Urchin Aminated Spinochromes against Herpes Simplex Virus Type 1
title_full Antiviral Potential of Sea Urchin Aminated Spinochromes against Herpes Simplex Virus Type 1
title_fullStr Antiviral Potential of Sea Urchin Aminated Spinochromes against Herpes Simplex Virus Type 1
title_full_unstemmed Antiviral Potential of Sea Urchin Aminated Spinochromes against Herpes Simplex Virus Type 1
title_short Antiviral Potential of Sea Urchin Aminated Spinochromes against Herpes Simplex Virus Type 1
title_sort antiviral potential of sea urchin aminated spinochromes against herpes simplex virus type 1
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7694471/
https://www.ncbi.nlm.nih.gov/pubmed/33167501
http://dx.doi.org/10.3390/md18110550
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