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Chromosome End Diversification in Sciarid Flies
Background: Dipterans exhibit a remarkable diversity of chromosome end structures in contrast to the conserved system defined by telomerase and short repeats. Within dipteran families, structure of chromosome termini is usually conserved within genera. With the aim to assess whether or not the evolu...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7694509/ https://www.ncbi.nlm.nih.gov/pubmed/33167604 http://dx.doi.org/10.3390/cells9112425 |
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author | Gorab, Eduardo |
author_facet | Gorab, Eduardo |
author_sort | Gorab, Eduardo |
collection | PubMed |
description | Background: Dipterans exhibit a remarkable diversity of chromosome end structures in contrast to the conserved system defined by telomerase and short repeats. Within dipteran families, structure of chromosome termini is usually conserved within genera. With the aim to assess whether or not the evolutionary distance between genera implies chromosome end diversification, this report exploits two representatives of Sciaridae, Rhynchosciara americana, and Trichomegalosphys pubescens. Methods: Probes and plasmid microlibraries obtained by chromosome end microdissection, in situ hybridization, cloning, and sequencing are among the methodological approaches employed in this work. Results: The data argue for the existence of either specific terminal DNA sequences for each chromosome tip in T. pubescens, or sequences common to all chromosome ends but their extension does not allow detection by in situ hybridization. Both sciarid species share terminal sequences that are significantly underrepresented in chromosome ends of T. pubescens. Conclusions: The data suggest an unusual terminal structure in T. pubescens chromosomes compared to other dipterans investigated. A putative, evolutionary process of repetitive DNA expansion that acted differentially to shape chromosome ends of the two flies is also discussed. |
format | Online Article Text |
id | pubmed-7694509 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-76945092020-11-28 Chromosome End Diversification in Sciarid Flies Gorab, Eduardo Cells Article Background: Dipterans exhibit a remarkable diversity of chromosome end structures in contrast to the conserved system defined by telomerase and short repeats. Within dipteran families, structure of chromosome termini is usually conserved within genera. With the aim to assess whether or not the evolutionary distance between genera implies chromosome end diversification, this report exploits two representatives of Sciaridae, Rhynchosciara americana, and Trichomegalosphys pubescens. Methods: Probes and plasmid microlibraries obtained by chromosome end microdissection, in situ hybridization, cloning, and sequencing are among the methodological approaches employed in this work. Results: The data argue for the existence of either specific terminal DNA sequences for each chromosome tip in T. pubescens, or sequences common to all chromosome ends but their extension does not allow detection by in situ hybridization. Both sciarid species share terminal sequences that are significantly underrepresented in chromosome ends of T. pubescens. Conclusions: The data suggest an unusual terminal structure in T. pubescens chromosomes compared to other dipterans investigated. A putative, evolutionary process of repetitive DNA expansion that acted differentially to shape chromosome ends of the two flies is also discussed. MDPI 2020-11-05 /pmc/articles/PMC7694509/ /pubmed/33167604 http://dx.doi.org/10.3390/cells9112425 Text en © 2020 by the author. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Gorab, Eduardo Chromosome End Diversification in Sciarid Flies |
title | Chromosome End Diversification in Sciarid Flies |
title_full | Chromosome End Diversification in Sciarid Flies |
title_fullStr | Chromosome End Diversification in Sciarid Flies |
title_full_unstemmed | Chromosome End Diversification in Sciarid Flies |
title_short | Chromosome End Diversification in Sciarid Flies |
title_sort | chromosome end diversification in sciarid flies |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7694509/ https://www.ncbi.nlm.nih.gov/pubmed/33167604 http://dx.doi.org/10.3390/cells9112425 |
work_keys_str_mv | AT gorabeduardo chromosomeenddiversificationinsciaridflies |