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Chromosome End Diversification in Sciarid Flies

Background: Dipterans exhibit a remarkable diversity of chromosome end structures in contrast to the conserved system defined by telomerase and short repeats. Within dipteran families, structure of chromosome termini is usually conserved within genera. With the aim to assess whether or not the evolu...

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Autor principal: Gorab, Eduardo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7694509/
https://www.ncbi.nlm.nih.gov/pubmed/33167604
http://dx.doi.org/10.3390/cells9112425
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author Gorab, Eduardo
author_facet Gorab, Eduardo
author_sort Gorab, Eduardo
collection PubMed
description Background: Dipterans exhibit a remarkable diversity of chromosome end structures in contrast to the conserved system defined by telomerase and short repeats. Within dipteran families, structure of chromosome termini is usually conserved within genera. With the aim to assess whether or not the evolutionary distance between genera implies chromosome end diversification, this report exploits two representatives of Sciaridae, Rhynchosciara americana, and Trichomegalosphys pubescens. Methods: Probes and plasmid microlibraries obtained by chromosome end microdissection, in situ hybridization, cloning, and sequencing are among the methodological approaches employed in this work. Results: The data argue for the existence of either specific terminal DNA sequences for each chromosome tip in T. pubescens, or sequences common to all chromosome ends but their extension does not allow detection by in situ hybridization. Both sciarid species share terminal sequences that are significantly underrepresented in chromosome ends of T. pubescens. Conclusions: The data suggest an unusual terminal structure in T. pubescens chromosomes compared to other dipterans investigated. A putative, evolutionary process of repetitive DNA expansion that acted differentially to shape chromosome ends of the two flies is also discussed.
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spelling pubmed-76945092020-11-28 Chromosome End Diversification in Sciarid Flies Gorab, Eduardo Cells Article Background: Dipterans exhibit a remarkable diversity of chromosome end structures in contrast to the conserved system defined by telomerase and short repeats. Within dipteran families, structure of chromosome termini is usually conserved within genera. With the aim to assess whether or not the evolutionary distance between genera implies chromosome end diversification, this report exploits two representatives of Sciaridae, Rhynchosciara americana, and Trichomegalosphys pubescens. Methods: Probes and plasmid microlibraries obtained by chromosome end microdissection, in situ hybridization, cloning, and sequencing are among the methodological approaches employed in this work. Results: The data argue for the existence of either specific terminal DNA sequences for each chromosome tip in T. pubescens, or sequences common to all chromosome ends but their extension does not allow detection by in situ hybridization. Both sciarid species share terminal sequences that are significantly underrepresented in chromosome ends of T. pubescens. Conclusions: The data suggest an unusual terminal structure in T. pubescens chromosomes compared to other dipterans investigated. A putative, evolutionary process of repetitive DNA expansion that acted differentially to shape chromosome ends of the two flies is also discussed. MDPI 2020-11-05 /pmc/articles/PMC7694509/ /pubmed/33167604 http://dx.doi.org/10.3390/cells9112425 Text en © 2020 by the author. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Gorab, Eduardo
Chromosome End Diversification in Sciarid Flies
title Chromosome End Diversification in Sciarid Flies
title_full Chromosome End Diversification in Sciarid Flies
title_fullStr Chromosome End Diversification in Sciarid Flies
title_full_unstemmed Chromosome End Diversification in Sciarid Flies
title_short Chromosome End Diversification in Sciarid Flies
title_sort chromosome end diversification in sciarid flies
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7694509/
https://www.ncbi.nlm.nih.gov/pubmed/33167604
http://dx.doi.org/10.3390/cells9112425
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