Cargando…
Lycium barbarum Polysaccharide Suppresses Expression of Fibrotic Proteins in Primary Human Corneal Fibroblasts
(1) Objective: To study the anti-fibrotic effects of Lycium barbarum polysaccharides (LBP) on corneal stromal fibroblasts and assess LBP’s effect on cell viability. (2) Methods: Primary human corneal keratocytes of passage 3 to 6 were used for all experiments. Cells are pretreated with LBP solution...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7694544/ https://www.ncbi.nlm.nih.gov/pubmed/33171906 http://dx.doi.org/10.3390/jcm9113572 |
_version_ | 1783615000973148160 |
---|---|
author | Kwok, Sum Sum Wong, Francisca Siu-Yin Shih, Kendrick Co Chan, Yau-Kei Bu, Yashan Chan, Tommy Chung-Yan Ng, Alex Lap-Ki Lo, Amy Cheuk-Yin Tong, Louis Yam, Gary Hin-Fai Jhanji, Vishal |
author_facet | Kwok, Sum Sum Wong, Francisca Siu-Yin Shih, Kendrick Co Chan, Yau-Kei Bu, Yashan Chan, Tommy Chung-Yan Ng, Alex Lap-Ki Lo, Amy Cheuk-Yin Tong, Louis Yam, Gary Hin-Fai Jhanji, Vishal |
author_sort | Kwok, Sum Sum |
collection | PubMed |
description | (1) Objective: To study the anti-fibrotic effects of Lycium barbarum polysaccharides (LBP) on corneal stromal fibroblasts and assess LBP’s effect on cell viability. (2) Methods: Primary human corneal keratocytes of passage 3 to 6 were used for all experiments. Cells are pretreated with LBP solution for 24 h and then transforming growth factor beta 1 (TGFβ1) for 48 h and collected for experiments. Fibrotic protein analysis was performed using immunofluorescence and Western blot. The effect of LBP on cell viability was assessed using the MTS assay. (3) Results: LBP significantly reduced the expression of fibrotic proteins, including α-SMA and extracellular matrix proteins (collagen type I and III). LBP significantly decreased the viability of myofibroblasts but not the fibroblasts. Conclusions: In this study, LBP was effective in the prevention of fibrosis gene expression. Further studies to assess the underlying mechanism and pharmacological properties will facilitate the formation of a topical LBP solution for in vivo studies. |
format | Online Article Text |
id | pubmed-7694544 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-76945442020-11-28 Lycium barbarum Polysaccharide Suppresses Expression of Fibrotic Proteins in Primary Human Corneal Fibroblasts Kwok, Sum Sum Wong, Francisca Siu-Yin Shih, Kendrick Co Chan, Yau-Kei Bu, Yashan Chan, Tommy Chung-Yan Ng, Alex Lap-Ki Lo, Amy Cheuk-Yin Tong, Louis Yam, Gary Hin-Fai Jhanji, Vishal J Clin Med Article (1) Objective: To study the anti-fibrotic effects of Lycium barbarum polysaccharides (LBP) on corneal stromal fibroblasts and assess LBP’s effect on cell viability. (2) Methods: Primary human corneal keratocytes of passage 3 to 6 were used for all experiments. Cells are pretreated with LBP solution for 24 h and then transforming growth factor beta 1 (TGFβ1) for 48 h and collected for experiments. Fibrotic protein analysis was performed using immunofluorescence and Western blot. The effect of LBP on cell viability was assessed using the MTS assay. (3) Results: LBP significantly reduced the expression of fibrotic proteins, including α-SMA and extracellular matrix proteins (collagen type I and III). LBP significantly decreased the viability of myofibroblasts but not the fibroblasts. Conclusions: In this study, LBP was effective in the prevention of fibrosis gene expression. Further studies to assess the underlying mechanism and pharmacological properties will facilitate the formation of a topical LBP solution for in vivo studies. MDPI 2020-11-06 /pmc/articles/PMC7694544/ /pubmed/33171906 http://dx.doi.org/10.3390/jcm9113572 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kwok, Sum Sum Wong, Francisca Siu-Yin Shih, Kendrick Co Chan, Yau-Kei Bu, Yashan Chan, Tommy Chung-Yan Ng, Alex Lap-Ki Lo, Amy Cheuk-Yin Tong, Louis Yam, Gary Hin-Fai Jhanji, Vishal Lycium barbarum Polysaccharide Suppresses Expression of Fibrotic Proteins in Primary Human Corneal Fibroblasts |
title | Lycium barbarum Polysaccharide Suppresses Expression of Fibrotic Proteins in Primary Human Corneal Fibroblasts |
title_full | Lycium barbarum Polysaccharide Suppresses Expression of Fibrotic Proteins in Primary Human Corneal Fibroblasts |
title_fullStr | Lycium barbarum Polysaccharide Suppresses Expression of Fibrotic Proteins in Primary Human Corneal Fibroblasts |
title_full_unstemmed | Lycium barbarum Polysaccharide Suppresses Expression of Fibrotic Proteins in Primary Human Corneal Fibroblasts |
title_short | Lycium barbarum Polysaccharide Suppresses Expression of Fibrotic Proteins in Primary Human Corneal Fibroblasts |
title_sort | lycium barbarum polysaccharide suppresses expression of fibrotic proteins in primary human corneal fibroblasts |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7694544/ https://www.ncbi.nlm.nih.gov/pubmed/33171906 http://dx.doi.org/10.3390/jcm9113572 |
work_keys_str_mv | AT kwoksumsum lyciumbarbarumpolysaccharidesuppressesexpressionoffibroticproteinsinprimaryhumancornealfibroblasts AT wongfranciscasiuyin lyciumbarbarumpolysaccharidesuppressesexpressionoffibroticproteinsinprimaryhumancornealfibroblasts AT shihkendrickco lyciumbarbarumpolysaccharidesuppressesexpressionoffibroticproteinsinprimaryhumancornealfibroblasts AT chanyaukei lyciumbarbarumpolysaccharidesuppressesexpressionoffibroticproteinsinprimaryhumancornealfibroblasts AT buyashan lyciumbarbarumpolysaccharidesuppressesexpressionoffibroticproteinsinprimaryhumancornealfibroblasts AT chantommychungyan lyciumbarbarumpolysaccharidesuppressesexpressionoffibroticproteinsinprimaryhumancornealfibroblasts AT ngalexlapki lyciumbarbarumpolysaccharidesuppressesexpressionoffibroticproteinsinprimaryhumancornealfibroblasts AT loamycheukyin lyciumbarbarumpolysaccharidesuppressesexpressionoffibroticproteinsinprimaryhumancornealfibroblasts AT tonglouis lyciumbarbarumpolysaccharidesuppressesexpressionoffibroticproteinsinprimaryhumancornealfibroblasts AT yamgaryhinfai lyciumbarbarumpolysaccharidesuppressesexpressionoffibroticproteinsinprimaryhumancornealfibroblasts AT jhanjivishal lyciumbarbarumpolysaccharidesuppressesexpressionoffibroticproteinsinprimaryhumancornealfibroblasts |