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Assessment of the Growth and Reproductive Performance of Cloned Pietrain Boars

SIMPLE SUMMARY: Somatic cell nuclear transfer (SCNT) is a potential and promising technique for preserving and enlarging superior livestock genetic resources. Here, we investigated the effects of cloning on Pietrain boars, which are widely used to produce lean-type swine because of their high growth...

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Autores principales: Shi, Junsong, Tan, Baohua, Luo, Lvhua, Li, Zicong, Hong, Linjun, Yang, Jie, Cai, Gengyuan, Zheng, Enqin, Wu, Zhenfang, Gu, Ting
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7694642/
https://www.ncbi.nlm.nih.gov/pubmed/33171943
http://dx.doi.org/10.3390/ani10112053
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author Shi, Junsong
Tan, Baohua
Luo, Lvhua
Li, Zicong
Hong, Linjun
Yang, Jie
Cai, Gengyuan
Zheng, Enqin
Wu, Zhenfang
Gu, Ting
author_facet Shi, Junsong
Tan, Baohua
Luo, Lvhua
Li, Zicong
Hong, Linjun
Yang, Jie
Cai, Gengyuan
Zheng, Enqin
Wu, Zhenfang
Gu, Ting
author_sort Shi, Junsong
collection PubMed
description SIMPLE SUMMARY: Somatic cell nuclear transfer (SCNT) is a potential and promising technique for preserving and enlarging superior livestock genetic resources. Here, we investigated the effects of cloning on Pietrain boars, which are widely used to produce lean-type swine because of their high growth rate and lean fat rate. Our data revealed that SCNT manipulation caused some defects in growth performance, but the clones had normal semen quality and reproductive performance. Furthermore, the progeny of clones exhibited better growth performance than non-clones. These results indicated that the cloning of superior boars can be applied to produce more commercial pigs with increased economic benefit in practical breeding and production. ABSTRACT: How to maximize the use of the genetic merits of the high-ranking boars (also called superior ones) is a considerable question in the pig breeding industry, considering the money and time spent on selection. Somatic cell nuclear transfer (SCNT) is one of the potential ways to answer the question, which can be applied to produce clones with genetic resources of superior boar for the production of commercial pigs. For practical application, it is essential to investigate whether the clones and their progeny keep behaving better than the “normal boars”, considering that in vitro culture and transfer manipulation would cause a series of harmful effects to the development of clones. In this study, 59,061 cloned embryos were transferred into 250 recipient sows to produce the clones of superior Pietrain boars. The growth performance of 12 clones and 36 non-clones and the semen quality of 19 clones and 28 non-clones were compared. The reproductive performance of 21 clones and 25 non-clones were also tested. Furthermore, we made a comparison in the growth performance between 466 progeny of the clones and 822 progeny of the non-clones. Our results showed that no significant difference in semen quality and reproductive performance was observed between the clones and the non-clones, although the clones grew slower and exhibited smaller body size than the non-clones. The F1 progeny of the clones showed a greater growth rate than the non-clones. Our results demonstrated through the large animal population showed that SCNT manipulation resulted in a low growth rate and small body size, but the clones could normally produce F1 progeny with excellent growth traits to bring more economic benefits. Therefore, SCNT could be effective in enlarging the merit genetics of the superior boars and increasing the economic benefits in pig reproduction and breeding.
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spelling pubmed-76946422020-11-28 Assessment of the Growth and Reproductive Performance of Cloned Pietrain Boars Shi, Junsong Tan, Baohua Luo, Lvhua Li, Zicong Hong, Linjun Yang, Jie Cai, Gengyuan Zheng, Enqin Wu, Zhenfang Gu, Ting Animals (Basel) Article SIMPLE SUMMARY: Somatic cell nuclear transfer (SCNT) is a potential and promising technique for preserving and enlarging superior livestock genetic resources. Here, we investigated the effects of cloning on Pietrain boars, which are widely used to produce lean-type swine because of their high growth rate and lean fat rate. Our data revealed that SCNT manipulation caused some defects in growth performance, but the clones had normal semen quality and reproductive performance. Furthermore, the progeny of clones exhibited better growth performance than non-clones. These results indicated that the cloning of superior boars can be applied to produce more commercial pigs with increased economic benefit in practical breeding and production. ABSTRACT: How to maximize the use of the genetic merits of the high-ranking boars (also called superior ones) is a considerable question in the pig breeding industry, considering the money and time spent on selection. Somatic cell nuclear transfer (SCNT) is one of the potential ways to answer the question, which can be applied to produce clones with genetic resources of superior boar for the production of commercial pigs. For practical application, it is essential to investigate whether the clones and their progeny keep behaving better than the “normal boars”, considering that in vitro culture and transfer manipulation would cause a series of harmful effects to the development of clones. In this study, 59,061 cloned embryos were transferred into 250 recipient sows to produce the clones of superior Pietrain boars. The growth performance of 12 clones and 36 non-clones and the semen quality of 19 clones and 28 non-clones were compared. The reproductive performance of 21 clones and 25 non-clones were also tested. Furthermore, we made a comparison in the growth performance between 466 progeny of the clones and 822 progeny of the non-clones. Our results showed that no significant difference in semen quality and reproductive performance was observed between the clones and the non-clones, although the clones grew slower and exhibited smaller body size than the non-clones. The F1 progeny of the clones showed a greater growth rate than the non-clones. Our results demonstrated through the large animal population showed that SCNT manipulation resulted in a low growth rate and small body size, but the clones could normally produce F1 progeny with excellent growth traits to bring more economic benefits. Therefore, SCNT could be effective in enlarging the merit genetics of the superior boars and increasing the economic benefits in pig reproduction and breeding. MDPI 2020-11-06 /pmc/articles/PMC7694642/ /pubmed/33171943 http://dx.doi.org/10.3390/ani10112053 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Shi, Junsong
Tan, Baohua
Luo, Lvhua
Li, Zicong
Hong, Linjun
Yang, Jie
Cai, Gengyuan
Zheng, Enqin
Wu, Zhenfang
Gu, Ting
Assessment of the Growth and Reproductive Performance of Cloned Pietrain Boars
title Assessment of the Growth and Reproductive Performance of Cloned Pietrain Boars
title_full Assessment of the Growth and Reproductive Performance of Cloned Pietrain Boars
title_fullStr Assessment of the Growth and Reproductive Performance of Cloned Pietrain Boars
title_full_unstemmed Assessment of the Growth and Reproductive Performance of Cloned Pietrain Boars
title_short Assessment of the Growth and Reproductive Performance of Cloned Pietrain Boars
title_sort assessment of the growth and reproductive performance of cloned pietrain boars
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7694642/
https://www.ncbi.nlm.nih.gov/pubmed/33171943
http://dx.doi.org/10.3390/ani10112053
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