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A Synthetic Bioactive Peptide Derived from the Asian Medicinal Plant Acacia catechu Binds to Dengue Virus and Inhibits Cell Entry

Dengue virus (DENV) infection has become a critically important globally prevalent infectious disease, especially in tropical and subtropical countries. Since neither currently exists, there is an urgent need for an effective vaccine to prevent, and a specific drug to treat DENV infection. Therapeut...

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Autores principales: Panya, Aussara, Sawasdee, Nunghathai, Songprakhon, Pucharee, Tragoolpua, Yingmanee, Rotarayanont, Siriphorn, Choowongkomon, Kiattawee, Yenchitsomanus, Pa-thai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7694746/
https://www.ncbi.nlm.nih.gov/pubmed/33172110
http://dx.doi.org/10.3390/v12111267
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author Panya, Aussara
Sawasdee, Nunghathai
Songprakhon, Pucharee
Tragoolpua, Yingmanee
Rotarayanont, Siriphorn
Choowongkomon, Kiattawee
Yenchitsomanus, Pa-thai
author_facet Panya, Aussara
Sawasdee, Nunghathai
Songprakhon, Pucharee
Tragoolpua, Yingmanee
Rotarayanont, Siriphorn
Choowongkomon, Kiattawee
Yenchitsomanus, Pa-thai
author_sort Panya, Aussara
collection PubMed
description Dengue virus (DENV) infection has become a critically important globally prevalent infectious disease, especially in tropical and subtropical countries. Since neither currently exists, there is an urgent need for an effective vaccine to prevent, and a specific drug to treat DENV infection. Therapeutic peptides represent an attractive alternative for development into anti-DENV drugs due to their safety and their diverse biological and chemical properties. We recently reported novel bioactive peptides extracted from the Asian medicinal plant Acacia catechu that efficiently inhibited all four DENV serotypes. In this study, we investigated the anti-DENV activity of a synthetic bioactive peptide derived from this plant. The most effective peptide (designated Pep-RTYM) inhibited DENV infection with a half-maximal inhibition concentration value of 7.9 μM. Time-of-addition study demonstrated that Pep-RTYM interacted with DENV particles and inhibited cellular entry. Pep-RTYM at 50 μM significantly reduced DENV production in Vero-kidney epithelial cells about 1000-fold, but it could decrease the virus production in Huh7 hepatocyte cells approximately 40-fold. Binding of Pep-RTYM to DENV particles may prevent virus interaction with cellular receptor and subsequent virus entry. This finding suggests a potential role of Pep-RTYM in the development of a novel anti-DENV drug.
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spelling pubmed-76947462020-11-28 A Synthetic Bioactive Peptide Derived from the Asian Medicinal Plant Acacia catechu Binds to Dengue Virus and Inhibits Cell Entry Panya, Aussara Sawasdee, Nunghathai Songprakhon, Pucharee Tragoolpua, Yingmanee Rotarayanont, Siriphorn Choowongkomon, Kiattawee Yenchitsomanus, Pa-thai Viruses Article Dengue virus (DENV) infection has become a critically important globally prevalent infectious disease, especially in tropical and subtropical countries. Since neither currently exists, there is an urgent need for an effective vaccine to prevent, and a specific drug to treat DENV infection. Therapeutic peptides represent an attractive alternative for development into anti-DENV drugs due to their safety and their diverse biological and chemical properties. We recently reported novel bioactive peptides extracted from the Asian medicinal plant Acacia catechu that efficiently inhibited all four DENV serotypes. In this study, we investigated the anti-DENV activity of a synthetic bioactive peptide derived from this plant. The most effective peptide (designated Pep-RTYM) inhibited DENV infection with a half-maximal inhibition concentration value of 7.9 μM. Time-of-addition study demonstrated that Pep-RTYM interacted with DENV particles and inhibited cellular entry. Pep-RTYM at 50 μM significantly reduced DENV production in Vero-kidney epithelial cells about 1000-fold, but it could decrease the virus production in Huh7 hepatocyte cells approximately 40-fold. Binding of Pep-RTYM to DENV particles may prevent virus interaction with cellular receptor and subsequent virus entry. This finding suggests a potential role of Pep-RTYM in the development of a novel anti-DENV drug. MDPI 2020-11-06 /pmc/articles/PMC7694746/ /pubmed/33172110 http://dx.doi.org/10.3390/v12111267 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Panya, Aussara
Sawasdee, Nunghathai
Songprakhon, Pucharee
Tragoolpua, Yingmanee
Rotarayanont, Siriphorn
Choowongkomon, Kiattawee
Yenchitsomanus, Pa-thai
A Synthetic Bioactive Peptide Derived from the Asian Medicinal Plant Acacia catechu Binds to Dengue Virus and Inhibits Cell Entry
title A Synthetic Bioactive Peptide Derived from the Asian Medicinal Plant Acacia catechu Binds to Dengue Virus and Inhibits Cell Entry
title_full A Synthetic Bioactive Peptide Derived from the Asian Medicinal Plant Acacia catechu Binds to Dengue Virus and Inhibits Cell Entry
title_fullStr A Synthetic Bioactive Peptide Derived from the Asian Medicinal Plant Acacia catechu Binds to Dengue Virus and Inhibits Cell Entry
title_full_unstemmed A Synthetic Bioactive Peptide Derived from the Asian Medicinal Plant Acacia catechu Binds to Dengue Virus and Inhibits Cell Entry
title_short A Synthetic Bioactive Peptide Derived from the Asian Medicinal Plant Acacia catechu Binds to Dengue Virus and Inhibits Cell Entry
title_sort synthetic bioactive peptide derived from the asian medicinal plant acacia catechu binds to dengue virus and inhibits cell entry
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7694746/
https://www.ncbi.nlm.nih.gov/pubmed/33172110
http://dx.doi.org/10.3390/v12111267
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