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Ibuprofen-Loaded Heparin Modified Thermosensitive Hydrogel for Inhibiting Excessive Inflammation and Promoting Wound Healing

Hydrogels have been investigated as ideal biomaterials for wound treatment owing to their ability to form a highly moist environment which accelerates cell migration and tissue regeneration for prompt wound healing. They can also be used as a drug carrier for local delivery, and are able to activate...

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Autores principales: Andrgie, Abegaz Tizazu, Darge, Haile Fentahun, Mekonnen, Tefera Worku, Birhan, Yihenew Simegniew, Hanurry, Endiries Yibru, Chou, Hsiao-Ying, Wang, Chih-Feng, Tsai, Hsieh-Chih, Yang, Jen Ming, Chang, Yen-Hsiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7694755/
https://www.ncbi.nlm.nih.gov/pubmed/33172099
http://dx.doi.org/10.3390/polym12112619
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author Andrgie, Abegaz Tizazu
Darge, Haile Fentahun
Mekonnen, Tefera Worku
Birhan, Yihenew Simegniew
Hanurry, Endiries Yibru
Chou, Hsiao-Ying
Wang, Chih-Feng
Tsai, Hsieh-Chih
Yang, Jen Ming
Chang, Yen-Hsiang
author_facet Andrgie, Abegaz Tizazu
Darge, Haile Fentahun
Mekonnen, Tefera Worku
Birhan, Yihenew Simegniew
Hanurry, Endiries Yibru
Chou, Hsiao-Ying
Wang, Chih-Feng
Tsai, Hsieh-Chih
Yang, Jen Ming
Chang, Yen-Hsiang
author_sort Andrgie, Abegaz Tizazu
collection PubMed
description Hydrogels have been investigated as ideal biomaterials for wound treatment owing to their ability to form a highly moist environment which accelerates cell migration and tissue regeneration for prompt wound healing. They can also be used as a drug carrier for local delivery, and are able to activate immune cells to enhance wound healing. Here, we developed heparin-conjugated poly(N-isopropylacrylamide), an injectable, in situ gel-forming polymer, and evaluated its use in wound healing. Ibuprofen was encapsulated into the hydrogel to help reduce pain and excessive inflammation during healing. In addition to in vitro studies, a BALB/c mice model was used to evaluate its effect on would healing and the secretion of inflammatory mediators. The in vitro assay confirmed that the ibuprofen released from the hydrogel dramatically reduced lipopolysaccharide-induced inflammation by suppressing the production of NO, PGE2 and TNF-α in RAW264.7 macrophages. Moreover, an in vivo wound healing assay was conducted by applying hydrogels to wounds on the backs of mice. The results showed that the ibuprofen-loaded hydrogel improved healing relative to the phosphate buffered saline group. This study indicates that ibuprofen loaded in an injectable hydrogel is a promising candidate for wound healing therapy.
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spelling pubmed-76947552020-11-28 Ibuprofen-Loaded Heparin Modified Thermosensitive Hydrogel for Inhibiting Excessive Inflammation and Promoting Wound Healing Andrgie, Abegaz Tizazu Darge, Haile Fentahun Mekonnen, Tefera Worku Birhan, Yihenew Simegniew Hanurry, Endiries Yibru Chou, Hsiao-Ying Wang, Chih-Feng Tsai, Hsieh-Chih Yang, Jen Ming Chang, Yen-Hsiang Polymers (Basel) Article Hydrogels have been investigated as ideal biomaterials for wound treatment owing to their ability to form a highly moist environment which accelerates cell migration and tissue regeneration for prompt wound healing. They can also be used as a drug carrier for local delivery, and are able to activate immune cells to enhance wound healing. Here, we developed heparin-conjugated poly(N-isopropylacrylamide), an injectable, in situ gel-forming polymer, and evaluated its use in wound healing. Ibuprofen was encapsulated into the hydrogel to help reduce pain and excessive inflammation during healing. In addition to in vitro studies, a BALB/c mice model was used to evaluate its effect on would healing and the secretion of inflammatory mediators. The in vitro assay confirmed that the ibuprofen released from the hydrogel dramatically reduced lipopolysaccharide-induced inflammation by suppressing the production of NO, PGE2 and TNF-α in RAW264.7 macrophages. Moreover, an in vivo wound healing assay was conducted by applying hydrogels to wounds on the backs of mice. The results showed that the ibuprofen-loaded hydrogel improved healing relative to the phosphate buffered saline group. This study indicates that ibuprofen loaded in an injectable hydrogel is a promising candidate for wound healing therapy. MDPI 2020-11-06 /pmc/articles/PMC7694755/ /pubmed/33172099 http://dx.doi.org/10.3390/polym12112619 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Andrgie, Abegaz Tizazu
Darge, Haile Fentahun
Mekonnen, Tefera Worku
Birhan, Yihenew Simegniew
Hanurry, Endiries Yibru
Chou, Hsiao-Ying
Wang, Chih-Feng
Tsai, Hsieh-Chih
Yang, Jen Ming
Chang, Yen-Hsiang
Ibuprofen-Loaded Heparin Modified Thermosensitive Hydrogel for Inhibiting Excessive Inflammation and Promoting Wound Healing
title Ibuprofen-Loaded Heparin Modified Thermosensitive Hydrogel for Inhibiting Excessive Inflammation and Promoting Wound Healing
title_full Ibuprofen-Loaded Heparin Modified Thermosensitive Hydrogel for Inhibiting Excessive Inflammation and Promoting Wound Healing
title_fullStr Ibuprofen-Loaded Heparin Modified Thermosensitive Hydrogel for Inhibiting Excessive Inflammation and Promoting Wound Healing
title_full_unstemmed Ibuprofen-Loaded Heparin Modified Thermosensitive Hydrogel for Inhibiting Excessive Inflammation and Promoting Wound Healing
title_short Ibuprofen-Loaded Heparin Modified Thermosensitive Hydrogel for Inhibiting Excessive Inflammation and Promoting Wound Healing
title_sort ibuprofen-loaded heparin modified thermosensitive hydrogel for inhibiting excessive inflammation and promoting wound healing
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7694755/
https://www.ncbi.nlm.nih.gov/pubmed/33172099
http://dx.doi.org/10.3390/polym12112619
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