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Analysis of Vancomycin-Resistant Enterococci in Hemato-Oncological Patients

Enterococci are important bacterial pathogens, and their significance is even greater in the case of vancomycin-resistant enterococci (VRE). The study analyzed the presence of VRE in the gastrointestinal tract (GIT) of hemato-oncological patients. Active screening using selective agars yielded VRE f...

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Autores principales: Hricová, Kristýna, Štosová, Taťána, Kučová, Pavla, Fišerová, Kateřina, Bardoň, Jan, Kolář, Milan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7694967/
https://www.ncbi.nlm.nih.gov/pubmed/33171728
http://dx.doi.org/10.3390/antibiotics9110785
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author Hricová, Kristýna
Štosová, Taťána
Kučová, Pavla
Fišerová, Kateřina
Bardoň, Jan
Kolář, Milan
author_facet Hricová, Kristýna
Štosová, Taťána
Kučová, Pavla
Fišerová, Kateřina
Bardoň, Jan
Kolář, Milan
author_sort Hricová, Kristýna
collection PubMed
description Enterococci are important bacterial pathogens, and their significance is even greater in the case of vancomycin-resistant enterococci (VRE). The study analyzed the presence of VRE in the gastrointestinal tract (GIT) of hemato-oncological patients. Active screening using selective agars yielded VRE for phenotypic and genotypic analyses. Isolated strains were identified with MALDI-TOF MS, (Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry) their susceptibility to antibiotics was tested, and resistance genes (vanA, vanB, vanC-1, vanC2-C3) and genes encoding virulence factors (asa1, gelE, cylA, esp, hyl) were detected. Pulsed-field gel electrophoresis was used to assess the relationship of the isolated strains. Over a period of three years, 103 VanA-type VRE were identified in 1405 hemato-oncological patients. The most frequently detected virulence factor was extracellular surface protein (84%), followed by hyaluronidase (40%). Unique restriction profiles were observed in 33% of strains; clonality was detected in 67% of isolates. The study found that 7% of hemato-oncological patients carried VRE in their GIT. In all cases, the species identified was Enterococcus faecium. No clone persisted for the entire 3-year study period. However, genetically different clusters were observed for shorter periods of time, no longer than eight months, with identical VRE spreading among patients.
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spelling pubmed-76949672020-11-28 Analysis of Vancomycin-Resistant Enterococci in Hemato-Oncological Patients Hricová, Kristýna Štosová, Taťána Kučová, Pavla Fišerová, Kateřina Bardoň, Jan Kolář, Milan Antibiotics (Basel) Article Enterococci are important bacterial pathogens, and their significance is even greater in the case of vancomycin-resistant enterococci (VRE). The study analyzed the presence of VRE in the gastrointestinal tract (GIT) of hemato-oncological patients. Active screening using selective agars yielded VRE for phenotypic and genotypic analyses. Isolated strains were identified with MALDI-TOF MS, (Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry) their susceptibility to antibiotics was tested, and resistance genes (vanA, vanB, vanC-1, vanC2-C3) and genes encoding virulence factors (asa1, gelE, cylA, esp, hyl) were detected. Pulsed-field gel electrophoresis was used to assess the relationship of the isolated strains. Over a period of three years, 103 VanA-type VRE were identified in 1405 hemato-oncological patients. The most frequently detected virulence factor was extracellular surface protein (84%), followed by hyaluronidase (40%). Unique restriction profiles were observed in 33% of strains; clonality was detected in 67% of isolates. The study found that 7% of hemato-oncological patients carried VRE in their GIT. In all cases, the species identified was Enterococcus faecium. No clone persisted for the entire 3-year study period. However, genetically different clusters were observed for shorter periods of time, no longer than eight months, with identical VRE spreading among patients. MDPI 2020-11-07 /pmc/articles/PMC7694967/ /pubmed/33171728 http://dx.doi.org/10.3390/antibiotics9110785 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hricová, Kristýna
Štosová, Taťána
Kučová, Pavla
Fišerová, Kateřina
Bardoň, Jan
Kolář, Milan
Analysis of Vancomycin-Resistant Enterococci in Hemato-Oncological Patients
title Analysis of Vancomycin-Resistant Enterococci in Hemato-Oncological Patients
title_full Analysis of Vancomycin-Resistant Enterococci in Hemato-Oncological Patients
title_fullStr Analysis of Vancomycin-Resistant Enterococci in Hemato-Oncological Patients
title_full_unstemmed Analysis of Vancomycin-Resistant Enterococci in Hemato-Oncological Patients
title_short Analysis of Vancomycin-Resistant Enterococci in Hemato-Oncological Patients
title_sort analysis of vancomycin-resistant enterococci in hemato-oncological patients
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7694967/
https://www.ncbi.nlm.nih.gov/pubmed/33171728
http://dx.doi.org/10.3390/antibiotics9110785
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