A Sensitive Reverse Transcription Loop-Mediated Isothermal Amplification Assay for Direct Visual Detection of SARS-CoV-2

A simple and rapid reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for the detection of SARS-CoV-2. The RT-LAMP assay was highly specific for SARS-CoV-2 and was able to detect one copy of transcribed SARS-CoV-2 RNA within 24 minutes. Assay validation perfor...

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Autores principales: Lau, Yee Ling, Ismail, Ilyiana Binti, Izati Binti Mustapa, Nur, Lai, Meng Yee, Tuan Soh, Tuan Suhaila, Hassan, Afifah Haji, Peariasamy, Kalaiarasu M., Lee, Yee Leng, Goh, Pik Pin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society of Tropical Medicine and Hygiene 2020
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7695063/
https://www.ncbi.nlm.nih.gov/pubmed/33098286
http://dx.doi.org/10.4269/ajtmh.20-1079
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author Lau, Yee Ling
Ismail, Ilyiana Binti
Izati Binti Mustapa, Nur
Lai, Meng Yee
Tuan Soh, Tuan Suhaila
Hassan, Afifah Haji
Peariasamy, Kalaiarasu M.
Lee, Yee Leng
Goh, Pik Pin
author_facet Lau, Yee Ling
Ismail, Ilyiana Binti
Izati Binti Mustapa, Nur
Lai, Meng Yee
Tuan Soh, Tuan Suhaila
Hassan, Afifah Haji
Peariasamy, Kalaiarasu M.
Lee, Yee Leng
Goh, Pik Pin
author_sort Lau, Yee Ling
collection PubMed
description A simple and rapid reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for the detection of SARS-CoV-2. The RT-LAMP assay was highly specific for SARS-CoV-2 and was able to detect one copy of transcribed SARS-CoV-2 RNA within 24 minutes. Assay validation performed using 50 positive and 32 negative clinical samples showed 100% sensitivity and specificity. The RT-LAMP would be valuable for clinical diagnosis and epidemiological surveillance of SARS-CoV-2 infection in resource-limited areas as it does not require the use of sophisticated and costly equipment.
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spelling pubmed-76950632020-11-30 A Sensitive Reverse Transcription Loop-Mediated Isothermal Amplification Assay for Direct Visual Detection of SARS-CoV-2 Lau, Yee Ling Ismail, Ilyiana Binti Izati Binti Mustapa, Nur Lai, Meng Yee Tuan Soh, Tuan Suhaila Hassan, Afifah Haji Peariasamy, Kalaiarasu M. Lee, Yee Leng Goh, Pik Pin Am J Trop Med Hyg Articles A simple and rapid reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for the detection of SARS-CoV-2. The RT-LAMP assay was highly specific for SARS-CoV-2 and was able to detect one copy of transcribed SARS-CoV-2 RNA within 24 minutes. Assay validation performed using 50 positive and 32 negative clinical samples showed 100% sensitivity and specificity. The RT-LAMP would be valuable for clinical diagnosis and epidemiological surveillance of SARS-CoV-2 infection in resource-limited areas as it does not require the use of sophisticated and costly equipment. The American Society of Tropical Medicine and Hygiene 2020-12 2020-10-23 /pmc/articles/PMC7695063/ /pubmed/33098286 http://dx.doi.org/10.4269/ajtmh.20-1079 Text en © The American Society of Tropical Medicine and Hygiene This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Articles
Lau, Yee Ling
Ismail, Ilyiana Binti
Izati Binti Mustapa, Nur
Lai, Meng Yee
Tuan Soh, Tuan Suhaila
Hassan, Afifah Haji
Peariasamy, Kalaiarasu M.
Lee, Yee Leng
Goh, Pik Pin
A Sensitive Reverse Transcription Loop-Mediated Isothermal Amplification Assay for Direct Visual Detection of SARS-CoV-2
title A Sensitive Reverse Transcription Loop-Mediated Isothermal Amplification Assay for Direct Visual Detection of SARS-CoV-2
title_full A Sensitive Reverse Transcription Loop-Mediated Isothermal Amplification Assay for Direct Visual Detection of SARS-CoV-2
title_fullStr A Sensitive Reverse Transcription Loop-Mediated Isothermal Amplification Assay for Direct Visual Detection of SARS-CoV-2
title_full_unstemmed A Sensitive Reverse Transcription Loop-Mediated Isothermal Amplification Assay for Direct Visual Detection of SARS-CoV-2
title_short A Sensitive Reverse Transcription Loop-Mediated Isothermal Amplification Assay for Direct Visual Detection of SARS-CoV-2
title_sort sensitive reverse transcription loop-mediated isothermal amplification assay for direct visual detection of sars-cov-2
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7695063/
https://www.ncbi.nlm.nih.gov/pubmed/33098286
http://dx.doi.org/10.4269/ajtmh.20-1079
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