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Punicalagin Regulates Key Processes Associated with Atherosclerosis in THP-1 Cellular Model

Atherosclerosis may lead to cardiovascular diseases (CVD), which are the primary cause of death globally. In addition to conventional therapeutics for CVD, use of nutraceuticals that prevents cholesterol deposition, reduce existing plaques and hence anti-atherosclerotic effects of nutraceuticals app...

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Autores principales: Almowallad, Sanaa, Huwait, Etimad, Al-Massabi, Rehab, Saddeek, Salma, Gauthaman, Kalamegam, Prola, Alexandre
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7695206/
https://www.ncbi.nlm.nih.gov/pubmed/33171640
http://dx.doi.org/10.3390/ph13110372
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author Almowallad, Sanaa
Huwait, Etimad
Al-Massabi, Rehab
Saddeek, Salma
Gauthaman, Kalamegam
Prola, Alexandre
author_facet Almowallad, Sanaa
Huwait, Etimad
Al-Massabi, Rehab
Saddeek, Salma
Gauthaman, Kalamegam
Prola, Alexandre
author_sort Almowallad, Sanaa
collection PubMed
description Atherosclerosis may lead to cardiovascular diseases (CVD), which are the primary cause of death globally. In addition to conventional therapeutics for CVD, use of nutraceuticals that prevents cholesterol deposition, reduce existing plaques and hence anti-atherosclerotic effects of nutraceuticals appeared to be promising. As such, in the present study we evaluated the beneficial effects of punicalagin, a phytochemical against an atherosclerotic cell model in vitro. Cytotoxicity assays were examined for 10 µM concentration of punicalagin on THP-1 macrophages. Real-time-polymerase chain reaction (RT-PCR) was used to analyze monocyte chemoattractant protein-1 (MCP-1) and Intercellular adhesion molecule (ICAM-1) expressions. Monocyte migration and cholesterol efflux assays were performed to investigate punicalagin’s further impact on the key steps of atherosclerosis. Cytotoxicity assays demonstrated no significant toxicity for punicalagin (10 µM) on THP-1 macrophages. Punicalagin inhibited the IFN-γ-induced overexpression of MCP-1 and ICAM-1 in macrophages by 10 fold and 3.49 fold, respectively, compared to the control. Punicalagin also reduced the MCP-1- mediated migration of monocytes by 28% compared to the control. Percentages of cellular cholesterol efflux were enhanced in presence or absence of IFN-γ by 88% and 84% compared to control with 58% and 62%, respectively. Punicalagin possesses anti-inflammatory and anti-atherosclerotic effects. Punicalagin also did not exhibit any cytotoxicity and therefore can be considered a safe and potential candidate for the treatment and prevention of atherosclerosis.
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spelling pubmed-76952062020-11-28 Punicalagin Regulates Key Processes Associated with Atherosclerosis in THP-1 Cellular Model Almowallad, Sanaa Huwait, Etimad Al-Massabi, Rehab Saddeek, Salma Gauthaman, Kalamegam Prola, Alexandre Pharmaceuticals (Basel) Article Atherosclerosis may lead to cardiovascular diseases (CVD), which are the primary cause of death globally. In addition to conventional therapeutics for CVD, use of nutraceuticals that prevents cholesterol deposition, reduce existing plaques and hence anti-atherosclerotic effects of nutraceuticals appeared to be promising. As such, in the present study we evaluated the beneficial effects of punicalagin, a phytochemical against an atherosclerotic cell model in vitro. Cytotoxicity assays were examined for 10 µM concentration of punicalagin on THP-1 macrophages. Real-time-polymerase chain reaction (RT-PCR) was used to analyze monocyte chemoattractant protein-1 (MCP-1) and Intercellular adhesion molecule (ICAM-1) expressions. Monocyte migration and cholesterol efflux assays were performed to investigate punicalagin’s further impact on the key steps of atherosclerosis. Cytotoxicity assays demonstrated no significant toxicity for punicalagin (10 µM) on THP-1 macrophages. Punicalagin inhibited the IFN-γ-induced overexpression of MCP-1 and ICAM-1 in macrophages by 10 fold and 3.49 fold, respectively, compared to the control. Punicalagin also reduced the MCP-1- mediated migration of monocytes by 28% compared to the control. Percentages of cellular cholesterol efflux were enhanced in presence or absence of IFN-γ by 88% and 84% compared to control with 58% and 62%, respectively. Punicalagin possesses anti-inflammatory and anti-atherosclerotic effects. Punicalagin also did not exhibit any cytotoxicity and therefore can be considered a safe and potential candidate for the treatment and prevention of atherosclerosis. MDPI 2020-11-08 /pmc/articles/PMC7695206/ /pubmed/33171640 http://dx.doi.org/10.3390/ph13110372 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Almowallad, Sanaa
Huwait, Etimad
Al-Massabi, Rehab
Saddeek, Salma
Gauthaman, Kalamegam
Prola, Alexandre
Punicalagin Regulates Key Processes Associated with Atherosclerosis in THP-1 Cellular Model
title Punicalagin Regulates Key Processes Associated with Atherosclerosis in THP-1 Cellular Model
title_full Punicalagin Regulates Key Processes Associated with Atherosclerosis in THP-1 Cellular Model
title_fullStr Punicalagin Regulates Key Processes Associated with Atherosclerosis in THP-1 Cellular Model
title_full_unstemmed Punicalagin Regulates Key Processes Associated with Atherosclerosis in THP-1 Cellular Model
title_short Punicalagin Regulates Key Processes Associated with Atherosclerosis in THP-1 Cellular Model
title_sort punicalagin regulates key processes associated with atherosclerosis in thp-1 cellular model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7695206/
https://www.ncbi.nlm.nih.gov/pubmed/33171640
http://dx.doi.org/10.3390/ph13110372
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