Cargando…

Plasma Levels of Occludin and Claudin-5 in Acute Stroke Are Correlated with the Type and Location of Stroke but Not with the Neurological State of Patients—Preliminary Data

The blood–brain barrier is the structure (BBB), which isolates the central nervous system from the external environmental. During a stroke, the BBB gets damaged, which is accompanied by changes in the concentrations and distributions of claudin-5, occludin, ZO-1, and other building blocks of the BBB...

Descripción completa

Detalles Bibliográficos
Autores principales: Lasek-Bal, Anetta, Kokot, Anna, Gendosz de Carrillo, Daria, Student, Sebastian, Pawletko, Katarzyna, Krzan, Aleksandra, Puz, Przemysław, Bal, Wiesław, Jędrzejowska-Szypułka, Halina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7695327/
https://www.ncbi.nlm.nih.gov/pubmed/33182224
http://dx.doi.org/10.3390/brainsci10110831
_version_ 1783615163596800000
author Lasek-Bal, Anetta
Kokot, Anna
Gendosz de Carrillo, Daria
Student, Sebastian
Pawletko, Katarzyna
Krzan, Aleksandra
Puz, Przemysław
Bal, Wiesław
Jędrzejowska-Szypułka, Halina
author_facet Lasek-Bal, Anetta
Kokot, Anna
Gendosz de Carrillo, Daria
Student, Sebastian
Pawletko, Katarzyna
Krzan, Aleksandra
Puz, Przemysław
Bal, Wiesław
Jędrzejowska-Szypułka, Halina
author_sort Lasek-Bal, Anetta
collection PubMed
description The blood–brain barrier is the structure (BBB), which isolates the central nervous system from the external environmental. During a stroke, the BBB gets damaged, which is accompanied by changes in the concentrations and distributions of claudin-5, occludin, ZO-1, and other building blocks of the BBB. The aim of this study was to assess the concentrations of selected components of the BBB—occludin, claudin-5, and zonulin (ZO-1)—and to define a potential relationship between the concentrations of these three substances and the type of stroke, the location and extent of the infarct focus, the neurological/functional status in the acute phase of the disease, and the patient’s clinical profile. Methods: In this prospective study, we qualified patients with first-in-life stroke. All patients were analyzed according to: the presence of comorbidities, type of stroke (OCSP), treatment type in the first day of hospitalization, hemorrhagic transformation of infarct focus (ECASS), neurological status on the first day of stroke (NIHSS), functional status (mRS) on the ninth day of disease. In all patients, the plasma concentrations of claudin-5, occludin, and ZO-1 on the first day of stroke were examined and next, the mean concentrations were analyzed and compared between subgroups created on the basis of demographical and clinical features. Results: The mean concentration of occludin was significantly higher in patients with partial anterior cerebral infarct (PACI) compared to patients with posterior cerebral infarct (POCI; 1.03 vs. 0.66 ng/mL; p = 0.009) and in patients with location of ischemic stroke in the carotid artery supply compared with in the vertebrobasilar supply (respectively: 1.036 vs. 0.660 ng/mL; p = 0.009). The mean concentration of claudin 5 was significantly higher in patients with PACI compared to patients with POCI (0.37 vs. 0.21 ng/mL; p = 0.011) and in patients with location of ischemic stroke in the carotid artery supply in comparison with vertebrobasilar supply (respectively: 0.373 vs. 0.249 ng/mL; p = 0.011). The differences in mean occludin and claudin 5 concentrations between female and male were statistically not significant, similarly between patients < 65 years and older. A significantly higher mean concentration of zonulin was observed in patients > 65 years of age compared to younger patients (0.59 vs. 0.48 ng/mL; p = 0.010) and in patients with arterial hypertension compared to patients without the disease (0.63 ng/mL vs. 0.26 ng/mL; p = 0.026). There were no statistically significant relationships between the concentration of occludin, claudin 5, and zonulin and the neurological status according to the NIHSS on the first day of stroke. Conclusions: The location of stroke in the anterior part of the brain’s blood supply is associated with high blood levels of occludin and claudin 5 in the acute phase of stroke. The blood concentration of occludin is significantly lower in lacunar stroke comparing to this in non-lacunar stroke. Old age and arterial hypertension correlate positively with the concentration of zonulin 1 in acute stroke. There is no relationship between the blood levels of occludin, claudin 5, and zonulin 1 on the first day of stroke and the neurological and functional status in the acute phase of the disease.
format Online
Article
Text
id pubmed-7695327
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-76953272020-11-28 Plasma Levels of Occludin and Claudin-5 in Acute Stroke Are Correlated with the Type and Location of Stroke but Not with the Neurological State of Patients—Preliminary Data Lasek-Bal, Anetta Kokot, Anna Gendosz de Carrillo, Daria Student, Sebastian Pawletko, Katarzyna Krzan, Aleksandra Puz, Przemysław Bal, Wiesław Jędrzejowska-Szypułka, Halina Brain Sci Article The blood–brain barrier is the structure (BBB), which isolates the central nervous system from the external environmental. During a stroke, the BBB gets damaged, which is accompanied by changes in the concentrations and distributions of claudin-5, occludin, ZO-1, and other building blocks of the BBB. The aim of this study was to assess the concentrations of selected components of the BBB—occludin, claudin-5, and zonulin (ZO-1)—and to define a potential relationship between the concentrations of these three substances and the type of stroke, the location and extent of the infarct focus, the neurological/functional status in the acute phase of the disease, and the patient’s clinical profile. Methods: In this prospective study, we qualified patients with first-in-life stroke. All patients were analyzed according to: the presence of comorbidities, type of stroke (OCSP), treatment type in the first day of hospitalization, hemorrhagic transformation of infarct focus (ECASS), neurological status on the first day of stroke (NIHSS), functional status (mRS) on the ninth day of disease. In all patients, the plasma concentrations of claudin-5, occludin, and ZO-1 on the first day of stroke were examined and next, the mean concentrations were analyzed and compared between subgroups created on the basis of demographical and clinical features. Results: The mean concentration of occludin was significantly higher in patients with partial anterior cerebral infarct (PACI) compared to patients with posterior cerebral infarct (POCI; 1.03 vs. 0.66 ng/mL; p = 0.009) and in patients with location of ischemic stroke in the carotid artery supply compared with in the vertebrobasilar supply (respectively: 1.036 vs. 0.660 ng/mL; p = 0.009). The mean concentration of claudin 5 was significantly higher in patients with PACI compared to patients with POCI (0.37 vs. 0.21 ng/mL; p = 0.011) and in patients with location of ischemic stroke in the carotid artery supply in comparison with vertebrobasilar supply (respectively: 0.373 vs. 0.249 ng/mL; p = 0.011). The differences in mean occludin and claudin 5 concentrations between female and male were statistically not significant, similarly between patients < 65 years and older. A significantly higher mean concentration of zonulin was observed in patients > 65 years of age compared to younger patients (0.59 vs. 0.48 ng/mL; p = 0.010) and in patients with arterial hypertension compared to patients without the disease (0.63 ng/mL vs. 0.26 ng/mL; p = 0.026). There were no statistically significant relationships between the concentration of occludin, claudin 5, and zonulin and the neurological status according to the NIHSS on the first day of stroke. Conclusions: The location of stroke in the anterior part of the brain’s blood supply is associated with high blood levels of occludin and claudin 5 in the acute phase of stroke. The blood concentration of occludin is significantly lower in lacunar stroke comparing to this in non-lacunar stroke. Old age and arterial hypertension correlate positively with the concentration of zonulin 1 in acute stroke. There is no relationship between the blood levels of occludin, claudin 5, and zonulin 1 on the first day of stroke and the neurological and functional status in the acute phase of the disease. MDPI 2020-11-09 /pmc/articles/PMC7695327/ /pubmed/33182224 http://dx.doi.org/10.3390/brainsci10110831 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lasek-Bal, Anetta
Kokot, Anna
Gendosz de Carrillo, Daria
Student, Sebastian
Pawletko, Katarzyna
Krzan, Aleksandra
Puz, Przemysław
Bal, Wiesław
Jędrzejowska-Szypułka, Halina
Plasma Levels of Occludin and Claudin-5 in Acute Stroke Are Correlated with the Type and Location of Stroke but Not with the Neurological State of Patients—Preliminary Data
title Plasma Levels of Occludin and Claudin-5 in Acute Stroke Are Correlated with the Type and Location of Stroke but Not with the Neurological State of Patients—Preliminary Data
title_full Plasma Levels of Occludin and Claudin-5 in Acute Stroke Are Correlated with the Type and Location of Stroke but Not with the Neurological State of Patients—Preliminary Data
title_fullStr Plasma Levels of Occludin and Claudin-5 in Acute Stroke Are Correlated with the Type and Location of Stroke but Not with the Neurological State of Patients—Preliminary Data
title_full_unstemmed Plasma Levels of Occludin and Claudin-5 in Acute Stroke Are Correlated with the Type and Location of Stroke but Not with the Neurological State of Patients—Preliminary Data
title_short Plasma Levels of Occludin and Claudin-5 in Acute Stroke Are Correlated with the Type and Location of Stroke but Not with the Neurological State of Patients—Preliminary Data
title_sort plasma levels of occludin and claudin-5 in acute stroke are correlated with the type and location of stroke but not with the neurological state of patients—preliminary data
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7695327/
https://www.ncbi.nlm.nih.gov/pubmed/33182224
http://dx.doi.org/10.3390/brainsci10110831
work_keys_str_mv AT lasekbalanetta plasmalevelsofoccludinandclaudin5inacutestrokearecorrelatedwiththetypeandlocationofstrokebutnotwiththeneurologicalstateofpatientspreliminarydata
AT kokotanna plasmalevelsofoccludinandclaudin5inacutestrokearecorrelatedwiththetypeandlocationofstrokebutnotwiththeneurologicalstateofpatientspreliminarydata
AT gendoszdecarrillodaria plasmalevelsofoccludinandclaudin5inacutestrokearecorrelatedwiththetypeandlocationofstrokebutnotwiththeneurologicalstateofpatientspreliminarydata
AT studentsebastian plasmalevelsofoccludinandclaudin5inacutestrokearecorrelatedwiththetypeandlocationofstrokebutnotwiththeneurologicalstateofpatientspreliminarydata
AT pawletkokatarzyna plasmalevelsofoccludinandclaudin5inacutestrokearecorrelatedwiththetypeandlocationofstrokebutnotwiththeneurologicalstateofpatientspreliminarydata
AT krzanaleksandra plasmalevelsofoccludinandclaudin5inacutestrokearecorrelatedwiththetypeandlocationofstrokebutnotwiththeneurologicalstateofpatientspreliminarydata
AT puzprzemysław plasmalevelsofoccludinandclaudin5inacutestrokearecorrelatedwiththetypeandlocationofstrokebutnotwiththeneurologicalstateofpatientspreliminarydata
AT balwiesław plasmalevelsofoccludinandclaudin5inacutestrokearecorrelatedwiththetypeandlocationofstrokebutnotwiththeneurologicalstateofpatientspreliminarydata
AT jedrzejowskaszypułkahalina plasmalevelsofoccludinandclaudin5inacutestrokearecorrelatedwiththetypeandlocationofstrokebutnotwiththeneurologicalstateofpatientspreliminarydata